Molecular requirements for C. elegans transgenerational epigenetic inheritance of pathogen avoidance DOI Creative Commons
Rachel Kaletsky, Rebecca S. Moore, Titas Sengupta

и другие.

eLife, Год журнала: 2025, Номер 14

Опубликована: Май 2, 2025

Bacteria are Caenorhabditis elegans’ food, and worms naturally attracted to many bacteria, including pathogenic Pseudomonas , preferring PA14 over laboratory Escherichia coli (OP50). Despite this natural attraction PA14, prior exposure causes the instead avoid PA14. This behavioral switch can happen quickly – even within duration of choice assay. We show that accurate assessment animals’ true first requires use a paralytic (azide) trap at their initial choice, preventing from avoidance assay period. previously discovered C. elegans 25°C plate-grown 20°C for 24 hr not only leads avoidance, but also four generations naïve progeny avoiding while other paradigms cause P0 and/or F1 avoidance. showed transgenerational (P0-F4) epigenetic is mediated by P11, small RNA produced P11 both necessary sufficient TEI learned highly expressed in our standard growth conditions (25°C on surfaces), conditions, suggesting reported failure observe F2-F4 likely due absence expression experimenters’ conditions. Additionally, we tested ~35 genes involvement pathogen The conservation multiple components sRNA mechanism across strains species suggests behavior be physiologically important wild

Язык: Английский

Molecular Requirements for C. elegans Transgenerational Epigenetic Inheritance of Pathogen Avoidance DOI Creative Commons
Rachel Kaletsky, Rebecca S. Moore, Titas Sengupta

и другие.

eLife, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

C. elegans’ major food source is bacteria, and worms are naturally attracted to many bacterial species, including pathogenic Pseudomonas ; in fact, prefer PA14 as well wild bacteria over the lab E. coli strain (OP50) standardly used laboratory setting. Many labs have shown that despite this natural attraction PA14, prior exposure causes instead avoid PA14. This behavioral switch can happen on a relatively fast time scale, even within duration of choice assay. Here we show accurate assessment animals’ true first requires use paralytic (azide) trap at their initial choice, prevent from avoidance assay period. We previously discovered elegans 25°C plate-grown 20°C for 24hrs not only leads these animals switching but also progeny avoiding naïve state, persists through F4 generation. Other types training cause P0 and/or F1 avoidance, do induce transgenerational (F2 beyond) inheritance. showed (P0-F4) learned mediated by P11, small RNA produced P11 both necessary sufficient epigenetic inheritance behavior. highly expressed our standard growth conditions (25°C surfaces), other conditions, suggesting reported failure observe F2-F4 most likely due absence expression experimenters’ conditions. Through mutant analyses, tested genes – germline regulators, uptake, interference/processing, chromatin modifiers, neuronal - involvement pathogen allowing us better understand molecular requirements process. found strains TEI least two P. vranovensis fluorescens 15, avoidance. The induced each species functions specific, distinct (Pv1 Pfs1 15 , respectively) either directly or indirectly reduce levels gene maco-1 which turn regulates daf-7 ASI neuron subsequent conservation multiple components mechanism across suggests behavior be functional physiologically important

Язык: Английский

Процитировано

0
Molecular Requirements for C. elegans Transgenerational Epigenetic Inheritance of Pathogen Avoidance DOI Open Access
Rachel Kaletsky, Rebecca S. Moore, Titas Sengupta

и другие.

Опубликована: Апрель 1, 2025

C. elegans’ major food source is bacteria, and worms are naturally attracted to many bacterial species, including pathogenic Pseudomonas ; in fact, prefer PA14 as well wild bacteria over the lab E. coli strain (OP50) standardly used laboratory setting. Many labs have shown that despite this natural attraction PA14, prior exposure causes instead avoid PA14. This behavioral switch can happen on a relatively fast time scale, even within duration of choice assay. Here we show accurate assessment animals’ true first requires use paralytic (azide) trap at their initial choice, prevent from avoidance assay period. We previously discovered elegans 25°C plate-grown 20°C for 24hrs not only leads these animals switching but also progeny avoiding naïve state, persists through F4 generation. Other types training cause P0 and/or F1 avoidance, do induce transgenerational (F2 beyond) inheritance. showed (P0-F4) learned mediated by P11, small RNA produced P11 both necessary sufficient epigenetic inheritance behavior. highly expressed our standard growth conditions (25°C surfaces), other conditions, suggesting reported failure observe F2-F4 most likely due absence expression experimenters’ conditions. Through mutant analyses, tested genes – germline regulators, uptake, interference/processing, chromatin modifiers, neuronal - involvement pathogen allowing us better understand molecular requirements process. found strains TEI least two P. vranovensis fluorescens 15, avoidance. The induced each species functions specific, distinct (Pv1 Pfs1 15 , respectively) either directly or indirectly reduce levels gene maco-1 which turn regulates daf-7 ASI neuron subsequent conservation multiple components mechanism across suggests behavior be functional physiologically important

Язык: Английский

Процитировано

0
Molecular requirements for C. elegans transgenerational epigenetic inheritance of pathogen avoidance DOI Creative Commons
Rachel Kaletsky, Rebecca S. Moore, Titas Sengupta

и другие.

eLife, Год журнала: 2025, Номер 14

Опубликована: Май 2, 2025

Bacteria are Caenorhabditis elegans’ food, and worms naturally attracted to many bacteria, including pathogenic Pseudomonas , preferring PA14 over laboratory Escherichia coli (OP50). Despite this natural attraction PA14, prior exposure causes the instead avoid PA14. This behavioral switch can happen quickly – even within duration of choice assay. We show that accurate assessment animals’ true first requires use a paralytic (azide) trap at their initial choice, preventing from avoidance assay period. previously discovered C. elegans 25°C plate-grown 20°C for 24 hr not only leads avoidance, but also four generations naïve progeny avoiding while other paradigms cause P0 and/or F1 avoidance. showed transgenerational (P0-F4) epigenetic is mediated by P11, small RNA produced P11 both necessary sufficient TEI learned highly expressed in our standard growth conditions (25°C on surfaces), conditions, suggesting reported failure observe F2-F4 likely due absence expression experimenters’ conditions. Additionally, we tested ~35 genes involvement pathogen The conservation multiple components sRNA mechanism across strains species suggests behavior be physiologically important wild

Язык: Английский

Процитировано

0