PLoS Biology, Год журнала: 2025, Номер 23(3), С. e3003014 - e3003014

Опубликована: Март 26, 2025

Sleep is a behavior that conserved throughout the animal kingdom. Yet, despite extensive studies in humans and models, exact function or functions of sleep remain(s) unknown. A complicating factor trying to elucidate complexity multiplicity neuronal circuits are involved regulation. It conceivable distinct sleep-regulating only specific aspects may underlie different functions. Thus, it would be beneficial assess contribution individual sleep’s putative The intricacy mammalian brain makes this task extremely difficult. However, fruit fly Drosophila melanogaster, with its simpler organization, available connectomics, unparalleled genetics, offers opportunity interrogate centers. In , neurons projecting dorsal fan-shaped body (dFB) have been proposed key regulators sleep, particularly homeostasis. We recently demonstrated most widely used genetic tool manipulate dFB neurons, 23E10-GAL4 driver, expresses 2 (VNC-SP neurons) located ventral nerve cord (VNC), analog vertebrate spinal cord. Since data supporting role for regulation obtained using 23E10-GAL4, unclear whether phenotypes reported these caused by VNC-SP cells. recent publication replicated our finding contains sleep-promoting VNC. also not at all, but suggestion was made tools dFB-specific very mild deprivation protocol. study, newly created driver line, we demonstrate optogenetic activation majority promotes show require stronger stimulation than cells promote sleep. addition, dFB-induced can consolidate short-term memory (STM) into long-term (LTM), suggesting benefit on circuit-specific. Finally, neurochemically heterogeneous divided 3 populations. Most express both glutamate acetylcholine, while minority one neurotransmitters. Importantly, do GABA, as previously suggested. Using neurotransmitter-specific tools, points cholinergic potent regulating

Язык: Английский