Spontaneous
brain
activity,
typically
investigated
using
resting-state
fMRI
(rsfMRI),
provides
a
measure
of
inter-areal
functional
connectivity
(RSFC).
Although
it
has
been
established
that
RSFC
is
non-stationary,
previous
dynamic
rsfMRI
studies
mainly
focused
on
revealing
the
spatial
characteristics
patterns,
but
temporal
relationship
between
these
patterns
remains
elusive.
Here
we
organization
characteristic
in
awake
rats
and
humans.
We
found
transitions
were
not
random
followed
specific
sequential
orders.
The
pattern
was
further
analyzed
graph
theory,
pivotal
identified.
This
study
demonstrated
spontaneous
activity
only
nonrandom
spatially,
also
temporally,
this
feature
well
conserved
rodents
These
results
offer
new
insights
into
understanding
spatiotemporal
dynamics
mammalian
brain.
Nature Communications,
Год журнала:
2019,
Номер
10(1)
Опубликована: Апрель 2, 2019
Neural
computations
occurring
simultaneously
in
multiple
cerebral
cortical
regions
are
critical
for
mediating
behaviors.
Progress
has
been
made
understanding
how
neural
activity
specific
contributes
to
behavior.
However,
there
is
a
lack
of
tools
that
allow
simultaneous
monitoring
and
perturbing
from
regions.
We
engineered
'See-Shells'-digitally
designed,
morphologically
realistic,
transparent
polymer
skulls
long-term
(>300
days)
optical
access
45
mm
Proceedings of the National Academy of Sciences,
Год журнала:
2017,
Номер
114(33)
Опубликована: Июль 31, 2017
Significance
The
hippocampus
with
its
dense
reciprocal
axonal
projections
to
and
from
cortex
is
widely
believed
mediate
numerous
cognitive
functions.
However,
it
unknown
whether
how
specific
hippocampal–cortical
activity
contributes
the
brain-wide
functional
connectivity.
Here,
we
use
optogenetics
fMRI
examine
excitatory
neural
initiated
in
dorsal
dentate
gyrus
of
propagates
modulates
resting-state
(rsfMRI)
We
discover
robust
propagation
at
low
frequency
(1
Hz),
which
enhances
interhemispheric
rsfMRI
connectivity
cortical
subcortical
visual
responses.
Our
findings
highlight
important
role
slow
oscillatory
driving
mediating
sensory
processing.
Journal of Neurophysiology,
Год журнала:
2019,
Номер
123(1), С. 356 - 366
Опубликована: Ноя. 20, 2019
Wide-field
calcium
imaging
is
often
used
to
measure
brain
dynamics
in
behaving
mice.
With
a
large
field
of
view
and
high
sampling
rate,
wide-field
can
monitor
activity
from
several
distant
cortical
areas
simultaneously,
revealing
interactions.
Interpretation
images
complicated,
however,
by
the
absorption
light
hemoglobin,
which
substantially
affect
measured
fluorescence.
One
approach
separating
hemodynamics
signals
use
multiwavelength
backscatter
recordings
hemoglobin.
Following
this
approach,
we
develop
spatially
detailed
regression-based
method
estimate
hemodynamics.
This
Spatial
Model
based
on
linear
form
Beer–Lambert
relationship
but
fit
at
every
pixel
image
does
not
rely
estimation
physical
parameters.
In
awake
mice
three
transgenic
lines,
offers
improved
separation
changes
GCaMP
The
improvement
pronounced
near
blood
vessels
and,
contrast
with
equations,
remove
vascular
artifacts
along
sagittal
midline
general
permits
more
accurate
fluorescence-based
determination
neuronal
across
cortex.
NEW
&
NOTEWORTHY
paper
addresses
well-known
strong
source
contamination
calcium-imaging
data:
To
guide
researchers
toward
best
separate
hemodynamics,
compare
performance
methods
commonly
mouse
lines
present
novel
regression
model
that
outperforms
other
techniques
consider.
Journal of Neuroscience,
Год журнала:
2021,
Номер
41(19), С. 4160 - 4168
Опубликована: Апрель 23, 2021
The
brain
functions
through
coordinated
activity
among
distributed
regions.
Wide-field
calcium
imaging,
combined
with
improved
genetically
encoded
indicators,
allows
sufficient
signal-to-noise
ratio
and
spatiotemporal
resolution
to
afford
a
unique
opportunity
capture
cortex-wide
dynamics
on
moment-by-moment
basis
in
behaving
animals.
Recent
applications
of
this
approach
have
been
uncovering
cortical
at
unprecedented
scales
during
various
cognitive
processes,
ranging
from
relatively
simple
sensorimotor
integration
more
complex
decision-making
tasks.
In
review,
we
will
highlight
recent
scientific
advances
enabled
by
wide-field
imaging
mice.
We
then
summarize
several
technical
considerations
future
opportunities
for
uncover
large-scale
circuit
dynamics.
Cerebral Cortex,
Год журнала:
2021,
Номер
32(12), С. 2668 - 2687
Опубликована: Сен. 15, 2021
Motor
behavior
results
in
complex
exchanges
of
motor
and
sensory
information
across
cortical
regions.
Therefore,
fully
understanding
the
cerebral
cortex's
role
requires
a
mesoscopic-level
description
regions
engaged,
their
functional
interactions,
how
these
interactions
change
with
behavioral
state.
Mesoscopic
Ca2+
imaging
through
transparent
polymer
skulls
mice
reveals
elevated
activation
dorsal
cortex
during
locomotion.
Using
correlations
between
time
series
fluorescence
from
28
(nodes)
obtained
using
spatial
independent
component
analysis
(sICA),
we
examined
changes
connectivity
rest
to
locomotion
goal
state
that
facilitate
Both
transitions
show
marked
increases
correlation
among
most
nodes.
However,
once
steady
continued
is
reached,
many
nodes,
including
primary
somatosensory
decreases
correlations,
while
retrosplenial
anterior
nodes
secondary
increases.
These
highlight
cortex,
representing
on
return
rest.
