PLoS Biology,
Год журнала:
2023,
Номер
21(9), С. e3002301 - e3002301
Опубликована: Сен. 18, 2023
Motion
is
an
important
aspect
of
visual
information.
The
directions
motion
are
encoded
in
the
retina
by
direction-selective
ganglion
cells
(DSGCs).
ON-OFF
DSGCs
and
ON
co-stratify
with
starburst
amacrine
(SACs)
inner
plexiform
layer
depend
on
SACs
for
their
direction
selectivity.
J-type
retinal
(J-RGCs),
a
type
OFF
mouse
retina,
other
hand,
do
not
SACs,
how
selectivity
J-RGCs
emerges
has
been
understood.
Here,
we
report
that
both
excitatory
inhibitory
synaptic
inputs
to
(DS),
playing
more
role
DS
come
from
functional
connections
between
spatially
asymmetric.
Thus,
form
functionally
contacts
even
though
dendritic
arbors
show
little
overlap.
These
findings
underscore
need
look
beyond
neurons’
stratification
patterns
circuit
studies.
Our
results
also
highlight
critical
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Дек. 29, 2023
Visual
information
processing
is
sculpted
by
a
diverse
group
of
inhibitory
interneurons
in
the
retina
called
amacrine
cells.
Yet,
for
most
>60
cell
types,
molecular
identities
and
specialized
functional
attributes
remain
elusive.
Here,
we
developed
an
intersectional
genetic
strategy
to
target
wide-field
cells
(WACs)
mouse
that
co-express
transcription
factor
Bhlhe22
Kappa
Opioid
Receptor
(KOR;
B/K
WACs).
WACs
feature
straight,
unbranched
dendrites
spanning
over
0.5
mm
(∼15°
visual
angle)
produce
non-spiking
responses
either
light
increments
or
decrements.
Two-photon
dendritic
population
imaging
reveals
Ca
2+
signals
tuned
physical
orientations
WAC
dendrites,
signifying
robust
structure-function
alignment.
establish
divergent
connections
with
multiple
retinal
neurons,
including
unexpected
non-orientation-tuned
ganglion
bipolar
Our
work
sets
stage
future
comprehensive
investigations
enigmatic
neurons:
WACs.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2020,
Номер
unknown
Опубликована: Апрель 20, 2020
Abstract
Acetylcholine
(ACh)
is
a
key
neurotransmitter
that
plays
diverse
roles
in
many
parts
of
the
central
nervous
system,
including
retina.
However,
assessing
precise
spatiotemporal
dynamics
ACh
technically
challenging
and
whether
transmits
signals
via
rapid,
point-to-point
synaptic
mechanisms,
or
broader-scale
‘non-synaptic’
mechanisms
has
been
difficult
to
ascertain.
Here,
we
examined
properties
cholinergic
transmission
at
individual
contacts
made
between
direction-selective
starburst
amacrine
cells
downstream
ganglion
Using
combination
electrophysiology,
serial
block-face
electron
microscopy,
two-photon
imaging,
demonstrate
signaling
bears
hallmarks
both
non-synaptic
forms
transmission.
co-activates
nicotinic
receptors
located
on
intersecting
dendrites
pairs
cells,
with
equal
efficiency
(non-synaptic)—
yet
retains
ability
generate
rapid
‘miniature’
currents
(∼1
ms
rise
times:
synaptic).
Fast
do
not
appear
depend
anatomically
well-defined
structures.
We
estimate
spread
limited
∼1-2
µ
m
from
its
sites
release,
which
may
help
starbursts
drive
local
responses
cell
dendrites.
Together,
our
results
establish
functional
architecture
for
synapse
propose
novel
motif
whereby
single
presynaptic
can
co-transmit
information
multiple
neurons
millisecond
timescale.
PLoS Biology,
Год журнала:
2023,
Номер
21(9), С. e3002301 - e3002301
Опубликована: Сен. 18, 2023
Motion
is
an
important
aspect
of
visual
information.
The
directions
motion
are
encoded
in
the
retina
by
direction-selective
ganglion
cells
(DSGCs).
ON-OFF
DSGCs
and
ON
co-stratify
with
starburst
amacrine
(SACs)
inner
plexiform
layer
depend
on
SACs
for
their
direction
selectivity.
J-type
retinal
(J-RGCs),
a
type
OFF
mouse
retina,
other
hand,
do
not
SACs,
how
selectivity
J-RGCs
emerges
has
been
understood.
Here,
we
report
that
both
excitatory
inhibitory
synaptic
inputs
to
(DS),
playing
more
role
DS
come
from
functional
connections
between
spatially
asymmetric.
Thus,
form
functionally
contacts
even
though
dendritic
arbors
show
little
overlap.
These
findings
underscore
need
look
beyond
neurons’
stratification
patterns
circuit
studies.
Our
results
also
highlight
critical
