Wiley Interdisciplinary Reviews Developmental Biology,
Год журнала:
2020,
Номер
10(5)
Опубликована: Сен. 16, 2020
Abstract
Single‐cell
RNA
sequencing
(scRNA‐seq)
has
emerged
as
a
powerful
tool
for
investigating
cell
states
and
functions
at
the
single‐cell
level.
It
greatly
revolutionized
transcriptomic
studies
in
many
life
science
research
fields,
such
neurobiology,
immunology,
developmental
biology.
With
fast
development
of
both
experimental
platforms
bioinformatics
approaches
over
past
decade,
scRNA‐seq
is
becoming
economically
feasible
experimentally
practical
biomedical
laboratories.
Drosophila
served
an
excellent
model
organism
dissecting
cellular
molecular
mechanisms
that
underlie
tissue
development,
adult
function,
disease,
aging.
The
recent
application
methods
to
tissues
led
number
exciting
discoveries.
In
this
review,
I
will
provide
summary
,
focusing
on
technical
biological
applications.
also
discuss
current
challenges
future
opportunities
making
new
discoveries
using
.
This
article
categorized
under:
Technologies
>
Analysis
Transcriptome
The
Drosophila
ventral
nerve
cord
(VNC)
receives
and
processes
descending
signals
from
the
brain
to
produce
a
variety
of
coordinated
locomotor
outputs.
It
also
integrates
sensory
information
periphery
sends
ascending
brain.
We
used
single-cell
transcriptomics
generate
an
unbiased
classification
cellular
diversity
in
VNC
five-day
old
adult
flies.
produced
atlas
26,000
high-quality
cells,
representing
more
than
100
transcriptionally
distinct
cell
types.
predominant
gene
signatures
defining
neuronal
types
reflect
shared
developmental
histories
based
on
neuroblast
which
cells
were
derived,
as
well
their
birth
order.
relative
position
along
anterior-posterior
axis
could
be
assigned
using
Hox
expression.
This
transcriptional
fly
will
valuable
resource
for
future
studies
neurodevelopment
behavior.
Recognition
of
environmental
cues
is
essential
for
the
survival
all
organisms.
Transcriptional
changes
occur
to
enable
generation
and
function
neural
circuits
underlying
sensory
perception.
To
gain
insight
into
these
changes,
we
generated
single-cell
transcriptomes
Drosophila
olfactory-
(ORNs),
thermo-,
hygro-sensory
neurons
at
an
early
developmental
adult
stage
using
single-nucleus
RNA
sequencing.
We
discovered
that
ORNs
maintain
expression
same
olfactory
receptors
across
development.
Using
receptor
computational
approaches,
matched
transcriptomic
clusters
corresponding
anatomically
physiologically
defined
neuron
types
multiple
stages.
found
cell-type-specific
partly
reflected
axon
trajectory
choices
in
development
modality
adults.
uncovered
stage-specific
genes
could
regulate
wiring
responses
distinct
ORN
types.
Collectively,
our
data
reveal
features
biology
provide
a
resource
future
studies
their
physiology.
Nucleic Acids Research,
Год журнала:
2021,
Номер
50(D1), С. D1010 - D1015
Опубликована: Окт. 8, 2021
Abstract
FlyAtlas
2
(flyatlas2.org)
is
a
database
and
web
application
for
studying
the
expression
of
genes
Drosophila
melanogaster
in
different
tissues
adults
larvae.
It
based
on
RNA-Seq
data,
incorporates
both
encoding
proteins
microRNAs.
We
have
now
completed
population
with
13
from
male
female
adults,
five
sex-specific
tissues,
eight
larval
tissues.
Larval
garland
cell
nephrocytes
also
been
included.
Major
enhancements
made
to
application.
First,
facility
has
added
‘Profile’
search
similar
pattern
tissue
as
query
gene.
This
may
help
establish
function
which
this
currently
unknown.
Second,
dedicated
midgut,
where
difference
gene
regions
pH
can
be
explored.
A
variety
further
improvements
interface
are
described.
