KLF9 Promotes Osteogenic Differentiation of Dental Stem Cells by Negatively Regulating Notch1 Mediated Signaling Pathway DOI Creative Commons
Xinyuan Zhao,

Zizhao Mai,

Lu Ye

и другие.

Frontiers in Bioscience-Landmark, Год журнала: 2023, Номер 28(5)

Опубликована: Май 6, 2023

Background: Human dental stem cells (DSCs) are excellent sources of for treating and craniofacial diseases. However, the mechanisms regulating DSC osteogenic differentiation still unclear. In this study, we aimed to determine role Krüppel-like factor 9 (KLF9) in biological functions DSCs explore underlying molecular mechanisms. Methods: Bioinformatic analyses, quantitative real-time polymerase chain reaction (qRT‒PCR) Western blotting were performed KLF9 level during DSCs. The effects depletion or overexpression on then evaluated. potential associated mineralized nodule-forming activities monitored via Alizarin red S staining analyses markers. regulatory effect Notch1 signaling pathway was analyzed by luciferase reporter assays. Results: mRNA expression consistently increased mesenchymal cell multiple public datasets, our qRT‒PCR data further validated finding. addition, promoted proliferation suppressed DSCs, while enforced potential. Mechanistically, negatively regulated Notch1-mediated directly binding promoter. More importantly, inhibition/overexpression partially rescued suppressive/enhancing depletion/overexpression indicating that is a functional downstream target KLF9. Conclusions: summary, results strongly demonstrate crucial transcription controls pathway.

Язык: Английский

Application of dental pulp stem cells for bone regeneration DOI Creative Commons
Ye Liu, Wei Xiong, Junyi Li

и другие.

Frontiers in Medicine, Год журнала: 2024, Номер 11

Опубликована: Фев. 29, 2024

Bone defects resulting from severe trauma, tumors, inflammation, and other factors are increasingly prevalent. Stem cell-based therapies have emerged as a promising alternative. Dental pulp stem cells (DPSCs), sourced dental pulp, garnered significant attention owing to their ready accessibility minimal collection-associated risks. Ongoing investigations into DPSCs revealed potential undergo osteogenic differentiation capacity secrete diverse array of ontogenetic components, such extracellular vesicles cell lysates. This comprehensive review article aims provide an in-depth analysis secretory emphasizing extraction techniques utilization while elucidating the intricate mechanisms governing bone regeneration. Furthermore, we explore merits demerits cell-free therapeutic modalities, well discuss prospects, opportunities, inherent challenges associated with DPSC therapy in context

Язык: Английский

Процитировано

9
Communication between endothelial cells and osteoblasts in regulation of bone homeostasis: Notch players DOI Creative Commons
Daria Perepletchikova, Anna Malashicheva

Stem Cell Research & Therapy, Год журнала: 2025, Номер 16(1)

Опубликована: Фев. 7, 2025

Endothelial cells coat blood vessels and release molecular signals to affect the fate of other cells. can adjust their behavior in response changing microenvironmental conditions. During bone regeneration, tissue factors that promote vessel growth. Notch is a key signaling regulates cell decisions many tissues plays an important role development homeostasis. Understanding interplay between angiogenesis osteogenesis currently focus research efforts order facilitate improve when needed. Our review explores cellular mechanisms including Notch-dependent endothelial-MSC communication drive osteogenesis-angiogenesis processes effects on remodeling repair.

Язык: Английский

Процитировано

1
Induction of osteogenesis by bone-targeted Notch activation DOI Creative Commons
Cong Xu,

Van Vuong Dinh,

Kai Kruse

и другие.

eLife, Год журнала: 2022, Номер 11

Опубликована: Фев. 4, 2022

Declining bone mass is associated with aging and osteoporosis, a disease characterized by progressive weakening of the skeleton increased fracture incidence. Growth lifelong homeostasis rely on interactions between different cell types including vascular cells mesenchymal stromal (MSCs). As these involve Notch signaling, we have explored whether treatment secreted ligand proteins can enhance osteogenesis in adult mice. We show that bone-targeting, high affinity version Delta-like 4, termed Dll4 (E12) , induces formation male mice without causing adverse effects other organs, which are known to intact signaling. Due lower surface thereby reduced retention same approach failed promote female ovariectomized but strongly enhanced trabecular combination parathyroid hormone. Single analysis indicates primarily acts MSCs has comparably minor osteoblasts, endothelial cells, or chondrocytes. propose activation signaling bone-targeted fusion might be therapeutically useful avoid detrimental Notch-dependent processes organs.

Язык: Английский

Процитировано

35
Jiangu granules ameliorate postmenopausal osteoporosis via rectifying bone homeostasis imbalance: A network pharmacology analysis based on multi-omics validation DOI Open Access

Chaoxiong Li,

Xiangquan Lin,

Lin Qin

и другие.

Phytomedicine, Год журнала: 2023, Номер 122, С. 155137 - 155137

Опубликована: Окт. 5, 2023

Язык: Английский

Процитировано

18
Exosomes: A New Hope for Angiogenesis-Mediated Bone Regeneration DOI Open Access
Martin Lange, Patrick Babczyk, Edda Tobiasch

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(10), С. 5204 - 5204

Опубликована: Май 10, 2024

Bone is a metabolically dynamic structure that generally remodeled throughout the lifetime of an individual but often causes problems with increasing age. A key player for bone development and homeostasis, also under pathological conditions, vasculature. This complex system arteries, veins, capillaries forms distinct structures where each subset endothelial cells has important functions. Starting basic process angiogenesis bone-specific blood vessel formation, coupled initial importance different vascular highlighted respect to how these are maintained or changed during aging, conditions. After exemplifying current knowledge on vasculature, this review will move exosomes, novel hotspot scientific research. Exosomes be introduced starting from their discovery via isolation procedures state-of-the-art characterization role in development, regeneration repair while summarizing underlying signal transduction pathways. With processes, especially mesenchymal stem cell-derived extracellular vesicles interest, which leads discussion patented applications update ongoing clinical trials. Taken together, provides overview vasculature regeneration, major focus exosomes influence intricate system, as they might useful therapeutic purposes near future.

Язык: Английский

Процитировано

7
Endothelial to mesenchymal Notch signaling regulates skeletal repair DOI Creative Commons
Sanja Novak,

Hitoshi Tanigawa,

Vijender Singh

и другие.

JCI Insight, Год журнала: 2024, Номер 9(12)

Опубликована: Май 23, 2024

We present a transcriptomic analysis that provides better understanding of regulatory mechanisms within the healthy and injured periosteum. The focus this work is on characterizing early events controlling bone healing during formation periosteal callus day 3 after fracture. Building our previous findings showing induced Notch1 signaling in osteoprogenitors leads to healing, we compared samples which Notch 1 intracellular domain overexpressed by stem/progenitor cells, with control intact fractured Molecular changes skeletal cells (SSPCs) other cell populations callus, including hematopoietic lineages, were determined. Notably, ligands differentially expressed endothelial mesenchymal populations, Dll4 restricted whereas Jag1 was populations. Targeted deletion using Cdh5CreER resulted negative effects fracture while SSPCs α-smooth muscle actin-CreER did not impact healing. Translating these observations into clinically relevant model revealed beneficial delivering alongside osteogenic inducer, BMP2. These provide insights periosteum, paving way for novel translational approaches

Язык: Английский

Процитировано

7
Targeting type H vessels in bone‐related diseases DOI Creative Commons
Juan Xu,

Shuang‐jian He,

Tingting Xia

и другие.

Journal of Cellular and Molecular Medicine, Год журнала: 2024, Номер 28(4)

Опубликована: Фев. 1, 2024

Blood vessels are essential for bone development and metabolism. Type H in bone, named after their high expression of CD31 Endomucin (Emcn), have recently been reported to locate mainly the metaphysis, exhibit different molecular properties couple osteogenesis angiogenesis. A strong correlation between type metabolism is now well-recognized. The crosstalk osteoprogenitor cells also involved metabolism-related diseases such as osteoporosis, osteoarthritis, fracture healing defects. Targeting vessel formation may become a new approach managing variety diseases. This review highlighted roles bone-related summarized research attempts develop targeted intervention, which will help us gain better understanding potential value clinical application.

Язык: Английский

Процитировано

6
Notch signaling and fluid shear stress in regulating osteogenic differentiation DOI Creative Commons
Yuwen Zhao, Kiarra Richardson, Rui Yang

и другие.

Frontiers in Bioengineering and Biotechnology, Год журнала: 2022, Номер 10

Опубликована: Окт. 5, 2022

Osteoporosis is a common bone and metabolic disease that characterized by density loss microstructural degeneration. Human marrow-derived mesenchymal stem cells (hMSCs) are multipotent progenitor with the potential to differentiate into various cell types, including osteoblasts, chondrocytes, adipocytes, which have been utilized extensively in field of tissue engineering cell-based therapy. Although fluid shear stress plays an important role osteogenic differentiation, cellular molecular mechanisms underlying this effect remain poorly understood. Here, locked nucleic acid (LNA)/DNA nanobiosensor was exploited monitor mRNA gene expression hMSCs were exposed physiologically relevant examine regulatory Notch signaling during differentiation. First, effects on viability, proliferation, morphology, differentiation investigated compared. Our results showed modulates morphology depending applied duration. By incorporating LNA/DNA alkaline phosphatase (ALP) staining, we further regulating Pharmacological treatment disrupt investigate govern induced experimental provide convincing evidence supporting regulates through signaling. Inhibition mediates reduced ALP enzyme activity decreased Dll4 expression. In conclusion, our will add new information concerning under Further studies may elucidate mechanosensitive

Язык: Английский

Процитировано

21
Sphingosine-1-phosphate promotes osteogenesis by stimulating osteoblast growth and neovascularization in a vascular endothelial growth factor–dependent manner DOI Creative Commons
Annalena Wille,

Sarah Weske,

Karin von Wnuck Lipinski

и другие.

Journal of Bone and Mineral Research, Год журнала: 2024, Номер 39(3), С. 357 - 372

Опубликована: Янв. 24, 2024

Sphingosine-1-phosphate (S1P) plays multiple roles in bone metabolism and regeneration. Here, we have identified a novel S1P-regulated osteoanabolic mechanism functionally connecting osteoblasts (OBs) to the highly specialized vasculature. We demonstrate that S1P/S1PR3 signaling OBs stimulates vascular endothelial growth factor (VEGFa) expression secretion promote an autocrine boost osteogenic H-type differentiation of marrow cells paracrine manner. VEGFa-neutralizing antibodies VEGF receptor inhibition by axitinib abrogated OB vitro formation male C57BL/6J vivo following S1P stimulation lyase inhibition, respectively. Pharmacological S1PR3 genetic deficiency suppressed VEGFa production, vitro, inhibited angiogenesis mice vivo. Together with previous work on functions S1PR2 S1PR3, our data suggest S1P-dependent regeneration employs several nonredundant positive feedback loops between The identification this yet unappreciated aspect may implications for regular homeostasis as well diseases where microvasculature is affected such age-related osteopenia posttraumatic

Язык: Английский

Процитировано

4
The pathogenesis and regulatory role of HIF-1 in rheumatoid arthritis DOI Creative Commons
Han Li,

Qi-Yang Wu,

Xu-Heng Teng

и другие.

Central European Journal of Immunology, Год журнала: 2023, Номер 48(4), С. 338 - 345

Опубликована: Янв. 1, 2023

AMA LI H, WU Q, TENG X, et al. The pathogenesis and regulatory role of HIF-1 in rheumatoid arthritis. Central European Journal Immunology. 2024. doi:10.5114/ceji.2023.134217. APA LI, H., WU, Q., TENG, X., Z., ZHU, M., & GU, C. (2024). https://doi.org/10.5114/ceji.2023.134217 Chicago HAN, QI-YANG XU-HENG ZHI-PENG MENG-TING CHAO-JIE BEN-JIA CHEN "The arthritis". Harvard C., CHEN, B., XIE, LUO, X. MLA HAN arthritis." Immunology, Vancouver Z, ZHU M, GU C

Язык: Английский

Процитировано

10