Peroxiredoxins—The Underrated Actors during Virus-Induced Oxidative Stress

Antioxidants, Год журнала: 2021, Номер 10(6), С. 977 - 977

Опубликована: Июнь 18, 2021

Enhanced production of reactive oxygen species (ROS) triggered by various stimuli, including viral infections, has attributed much attention in the past years. It been shown that different viruses cause acute or chronic diseases induce oxidative stress infected cells and dysregulate antioxidant its capacity. However, most studies focused on catalase superoxide dismutases, whereas a family peroxiredoxins (Prdx), effective peroxide scavengers, were given little no attention. In current review, we demonstrate scavenge hydrogen organic peroxides at their physiological concentrations cell compartments, unlike many other enzymes, discuss recycling. We also provide data regulation expression transcription factors, as they can be compared with imprint transcriptional machinery. Next, involvement transferring signals from ROS specific proteins promoting oxidation target cysteine groups, well briefly evidence nonenzymatic, chaperone, functions Prdx. Finally, give an account state research for viruses. These clearly show Prdx have not proper despite all achievements general redox biology.

Язык: Английский

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Yeast Protein Kinase A Isoforms: A Means of Encoding Specificity in the Response to Diverse Stress Conditions?

Biomolecules, Год журнала: 2022, Номер 12(7), С. 958 - 958

Опубликована: Июль 8, 2022

Eukaryotic cells have developed a complex circuitry of signalling molecules which monitor changes in their intra- and extracellular environments. One the most widely studied pathways is highly conserved cyclic AMP (cAMP)/protein kinase A (PKA) pathway, major glucose sensing circuit yeast Saccharomyces cerevisiae. PKA activity regulates diverse targets yeast, positively activating processes that are associated with rapid cell growth (e.g., fermentative metabolism, ribosome biogenesis division) negatively regulating slow growth, such as respiratory carbohydrate storage entry into stationary phase. As higher eukaryotes, has evolved complexity at level catalytic subunit, cerevisiae expresses three isoforms, denoted Tpk1-3. Despite evidence for isoform differences multiple biological processes, molecular basis specificity remains poorly defined, many studies continue to assume redundancy regards PKA-mediated regulation. canonically been shown play key role fine-tuning cellular response stressors; however, recent now begun interrogate requirement individual isoforms coordinating distinct steps stress pathways. In this review, we discuss known non-redundant functions Tpk subunits evolving picture how these establish different conditions.

Язык: Английский

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The interactome of the Bakers' yeast peroxiredoxin Tsa1 implicates it in the redox regulation of intermediary metabolism, glycolysis and zinc homeostasis

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 21, 2025

ABSTRACT Zinc (Zn) is an essential nutrient supporting a range of critical processes. In the yeast Saccharomyces cerevisiae , Zn deficiency induces transcriptional response mediated by Zap1 activator, which controls regulon ∼80 genes. A subset support zinc homeostasis promoting uptake and its distribution between compartments, while remainder mediate “adaptive response” to enhance fitness deficient cells. The peroxiredoxin Tsa1 Zap1-regulated adaptive factor for growth can function as antioxidant peroxidase, protein chaperone, or redox sensor: latter activity oxidizes associated proteins via relay mechanism. We previously reported that in cells, inhibits pyruvate kinase (Pyk1) conserve phosphoenolpyruvate aromatic amino acid synthesis. However, this regulation makes relatively minor contribution low zinc, suggesting targets other pathways important adaptation. Consistent with model, sensor was ZnD Using MBP-tagged version Tsa1, we identified redox-sensitive non-covalent interaction Pyk1, applied system identify multiple novel interacting partners. This interactome implicates processes including many Zn-dependent metabolic pathways. Interestingly, preferred target, strongly promoted oxidation activation domain 2, full activity. Our findings reveal posttranslational deficiency, overlain on interconnected Zap1-mediated response. Graphical abstract mediates adaptation glycolytic enzymes Pyk1 Fba1, supports activator AD2 domain.

Язык: Английский

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Cytosolic Peroxiredoxin TSA1 Influences Acetic Acid Metabolism and pH Homeostasis in Wine Yeasts

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 22, 2025

Acetic acid is a key metabolite in yeast fermentation, influencing wine quality through its role volatile acidity. In Saccharomyces cerevisiae, acetic production involves aldehyde dehydrogenases, primarily Ald6p during fermentation and Ald4p under respiratory conditions. However, the regulatory mechanisms of these enzymes throughout how they differ commonly used strains remain partially unclear. This study explores cytosolic peroxiredoxin Tsa1p as novel regulator metabolism. TSA1 gene deletion revealed strain-dependent effects on metabolism tolerance, showing reduced enhanced consumption laboratory media. Under respiration, Ald4p-driven production, which raises extracellular pH, was mitigated by absence Tsa1p. During decreased initial surge downregulating ALD6 transcription enzymatic activity. These findings establish metabolic potential target for modulating levels to manage acidity improve quality.

Язык: Английский

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Structural determinants of multimerization and dissociation in 2-Cys peroxiredoxin chaperone function

Structure, Год журнала: 2021, Номер 29(7), С. 640 - 654

Опубликована: Май 3, 2021

Peroxiredoxins (PRDXs) are abundant peroxidases present in all kingdoms of life. Recently, they have been shown to also carry out additional roles as molecular chaperones. To address this emerging supplementary function, review focuses on structural studies 2-Cys PRDX systems exhibiting chaperone activity. We provide a detailed understanding the current knowledge determinants underlying function PRDXs. Specifically, we describe mechanisms which may modulate their quaternary structure facilitate interactions with client proteins and how coordinated functions other Following an overview architecture, outline details presently best-characterized peroxiredoxins highlight common denominators. Finally, discuss remarkable similarities between PRDXs, small HSPs, J-domain-independent Hsp40 holdases terms dynamic equilibria low- high-molecular-weight oligomers.

Язык: Английский

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LEIA: NASA’s first biological mission on the lunar surface since 1972

Acta Astronautica, Год журнала: 2025, Номер 231, С. 148 - 152

Опубликована: Фев. 28, 2025

Язык: Английский

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Peroxiredoxin Tsa1 Regulates the Activity of Trehalose Metabolism‐Related Enzymes During Wine Yeast Biomass Propagation

Microbial Biotechnology, Год журнала: 2025, Номер 18(5)

Опубликована: Май 1, 2025

ABSTRACT Trehalose metabolism plays a crucial role in yeast stress tolerance during biomass propagation and dehydration, but its regulatory mechanisms under these industrial conditions remain incompletely understood. This study analyses the of an antioxidant enzyme, cytosolic peroxiredoxin Tsa1, modulating trehalose Saccharomyces cerevisiae wine strains production molasses. Through comparative three commercial genetic backgrounds (L2056, T73, EC1118), we demonstrate that TSA1 deletion generally leads to increased intracellular accumulation despite phenotypic variability among strains. Enzymatic assays revealed Tsa1 does not regulate synthesis by altering glycolytic/gluconeogenic flux through pyruvate kinase. However, resulted oxidation enzymes, as well enhanced activity trehalose‐6‐phosphate synthase trehalases Nth1 Ath1, suggesting involvement futile cycle degradation. Scaling up process semi‐industrial confirmed findings, with levels tsa1 ∆ mutant correlating desiccation resistance resulting biomass. These results highlight novel Tsa1‐dependent mechanism governing beyond canonical role. Understanding this pathway provides new insights into optimising for applications.

Язык: Английский

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Mechanisms and Strategies for Engineering Oxidative Stress Resistance in Saccharomyces cerevisiae

Chem & Bio Engineering, Год журнала: 2025, Номер unknown

Опубликована: Май 28, 2025

Язык: Английский

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SESTRINs: Emerging Dynamic Stress-Sensors in Metabolic and Environmental Health

Frontiers in Cell and Developmental Biology, Год журнала: 2020, Номер 8

Опубликована: Дек. 3, 2020

Proper timely management of various external and internal stresses is critical for metabolic redox homeostasis in mammals. In particular, dysregulation mechanistic target rapamycin complex (mTORC) triggered from stress accumulation reactive oxygen species (ROS) generated environmental genotoxic are well-known culprits leading to chronic disease conditions humans. Sestrins one the stress-responsive groups proteins, which solely have ability regulate both mTORC activity ROS levels cells, tissues organs. While originally reported as several p53 genes, recent studies further delineated roles this group stress-sensing proteins regulation insulin sensitivity, glucose fat metabolism, redox-function aging. review, we discuss that investigated manipulated Sestrins-mediated signaling pathways health. an emerging dynamic stress-sensor drawing a spotlight preventive or therapeutic mechanism stress-associated pathologies aging processes at same time.

Язык: Английский

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Protein kinase A controls yeast growth in visible light

BMC Biology, Год журнала: 2020, Номер 18(1)

Опубликована: Ноя. 16, 2020

Abstract Background A wide variety of photosynthetic and non-photosynthetic species sense respond to light, having developed protective mechanisms adapt damaging effects on DNA proteins. While the biology UV light-induced damage has been well studied, cellular responses stress from visible light (400–700 nm) remain poorly understood despite being a regular part life cycle many organisms. Here, we high-throughput method for measuring growth under used it screen sensitivity in yeast gene deletion collection. Results We found genes involved HOG pathway signaling, RNA polymerase II transcription, translation, diphthamide modifications translational elongation factor eEF2, oxidative response be required resistance. Reduced nuclear localization transcription Msn2 lower glycogen accumulation indicated higher protein kinase (cAMP-dependent kinase, PKA) activity light-sensitive strains. therefore an ectopic fluorescent PKA reporter mutants with constitutively altered show that repression is essential resistance light. Conclusion conclude photobiology multifaceted plays key role ability cells grow upon exposure. propose impacts evolution organisms have practical implications how are studied laboratory, or without illumination.

Язык: Английский

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