Large-scale neural recordings call for new insights to link brain and behavior

Nature Neuroscience, Год журнала: 2022, Номер 25(1), С. 11 - 19

Опубликована: Янв. 1, 2022

Язык: Английский

---

The emergence and influence of internal states

Neuron, Год журнала: 2022, Номер 110(16), С. 2545 - 2570

Опубликована: Май 27, 2022

Язык: Английский

---

Brain-wide representations of behavior spanning multiple timescales and states in C. elegans

Cell, Год журнала: 2023, Номер 186(19), С. 4134 - 4151.e31

Опубликована: Авг. 21, 2023

Changes in an animal's behavior and internal state are accompanied by widespread changes activity across its brain. However, how neurons the brain encode this is impacted poorly understood. We recorded brain-wide diverse motor programs of freely moving C. elegans built probabilistic models that explain each neuron encodes quantitative behavioral features. By determining identities neurons, we created atlas defined classes connectome behavior. Many have conjunctive representations multiple behaviors. Moreover, although many current actions, others integrate recent actions. behavior, identify these flexible nodes connectome. Our results provide a global map cell types

Язык: Английский

---

Dissecting the functional organization of the C. elegans serotonergic system at whole-brain scale

Cell, Год журнала: 2023, Номер 186(12), С. 2574 - 2592.e20

Опубликована: Май 15, 2023

Serotonin influences many aspects of animal behavior. But how serotonin acts on its diverse receptors across the brain to modulate global activity and behavior is unknown. Here, we examine release in C. elegans alters brain-wide induce foraging behaviors, like slow locomotion increased feeding. Comprehensive genetic analyses identify three core (MOD-1, SER-4, LGC-50) that upon others (SER-1, SER-5, SER-7) interact with them this SER-4 induces behavioral responses sudden increases release, whereas MOD-1 persistent release. Whole-brain imaging reveals widespread serotonin-associated dynamics, spanning networks. We map all sites receptor expression connectome, which, together synaptic connectivity, helps predict which neurons show activity. These results reveal at defined a connectome

Язык: Английский

---

A line attractor encoding a persistent internal state requires neuropeptide signaling

Cell, Год журнала: 2024, Номер 187(21), С. 5998 - 6015.e18

Опубликована: Авг. 26, 2024

Язык: Английский

---

Behavioral States

Genetics, Год журнала: 2020, Номер 216(2), С. 315 - 332

Опубликована: Окт. 1, 2020

' behavioral states, like those of other animals, are shaped by its immediate environment, past experiences, and internal factors. We here review the literature on

Язык: Английский

---

A neural circuit for flexible control of persistent behavioral states

eLife, Год журнала: 2021, Номер 10

Опубликована: Ноя. 18, 2021

To adapt to their environments, animals must generate behaviors that are closely aligned a rapidly changing sensory world. However, behavioral states such as foraging or courtship typically persist over long time scales ensure proper execution. It remains unclear how neural circuits persistent while maintaining the flexibility select among alternative when context changes. Here, we elucidate functional architecture of circuit controlling choice between roaming and dwelling states, which underlie exploration exploitation during in C. elegans. By imaging ensemble-level activity freely moving animals, identify stereotyped changes corresponding each state. Combining circuit-wide with genetic analysis, find mutual inhibition two antagonistic neuromodulatory systems underlies persistence exclusivity patterns observed Through machine learning analysis perturbations, processing neuron can transmit information about food odors both bias animal towards different contexts, giving rise context-appropriate state transitions. Our findings reveal potentially general enables flexible, sensory-driven control states.

Язык: Английский

---

Diverse states and stimuli tune olfactory receptor expression levels to modulate food-seeking behavior

eLife, Год журнала: 2022, Номер 11

Опубликована: Авг. 31, 2022

Animals must weigh competing needs and states to generate adaptive behavioral responses the environment. Sensorimotor circuits are thus tasked with integrating diverse external internal cues relevant these context-appropriate behaviors. However, mechanisms that underlie this integration largely unknown. Here, we show a wide range of stimuli converge upon single Caenorhabditis elegans olfactory neuron modulate food-seeking behavior. Using an unbiased ribotagging approach, find expression receptor genes in AWA is influenced by array stimuli, including feeding state, physiological stress, recent sensory cues. We identify odorants activate state-dependent receptors altered influences foraging. Further, dissect molecular neural circuit pathways through which information nutritional state integrated AWA. This reveals modular organization state-related signals arising from different cell types body on independently control chemoreceptor expression. The synthesis allows animals sensorimotor reflect animal's overall state. Our findings suggest general model sensory- transcriptional changes at periphery animals' meet their ongoing states.

Язык: Английский

---

Fast, efficient, and accurate neuro-imaging denoising via supervised deep learning

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Сен. 2, 2022

Volumetric functional imaging is widely used for recording neuron activities in vivo, but there exist tradeoffs between the quality of extracted calcium traces, speed, and laser power. While deep-learning methods have recently been applied to denoise images, their applications downstream analyses, such as recovering high-SNR limited. Further, these require temporally-sequential pre-registered data acquired at ultrafast rates. Here, we demonstrate a supervised deep-denoising method circumvent several applications, including whole-brain imaging, large-field-of-view freely moving animals, complex neurite structures C. elegans. Our framework has 30× smaller memory footprint, fast training inference (50-70 ms); it highly accurate generalizable, further, trained with only small, non-temporally-sequential, independently-acquired datasets (∼500 pairs images). We envision that will enable faster long-term experiments necessary study neuronal mechanisms many behaviors.

Язык: Английский

---

Multisite regulation integrates multimodal context in sensory circuits to control persistent behavioral states in C. elegans

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Май 26, 2023

Abstract Maintaining or shifting between behavioral states according to context is essential for animals implement fitness-promoting strategies. How the integration of internal state, past experience and sensory inputs orchestrates persistent multidimensional changes remains poorly understood. Here, we show that C. elegans integrates environmental temperature food availability over different timescales engage in dwelling, scanning, global glocal search strategies matching thermoregulatory feeding needs. Transition states, each case, involves regulating multiple processes including AFD FLP tonic neurons activity, neuropeptide expression downstream circuit responsiveness. State-specific FLP-6 FLP-5 signaling acts on a distributed set inhibitory GPCR(s) promote scanning search, respectively, bypassing dopamine glutamate-dependent state control. Integration multimodal via multisite regulation circuits might represent conserved regulatory logic flexible prioritization valence when operating transitions.

Язык: Английский

---