bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 3, 2024
Abstract
Patterning
of
cell
fates
is
central
to
embryonic
development,
tissue
homeostasis,
and
disease.
Quantitative
analysis
patterning
reveals
the
logic
by
which
cell-cell
interactions
orchestrate
changes
in
fate.
However,
it
challenging
quantify
when
graded
identity
occur
over
complex
4D
trajectories,
or
where
different
states
are
intermingled.
Furthermore,
comparing
patterns
across
multiple
individual
embryos,
tissues,
organoids
difficult
because
these
often
vary
shape
size.
Here
we
present
a
toolkit
computational
approaches
tackle
problems.
These
strategies
based
on
measuring
properties
each
relation
its
neighbours
patterning,
using
landmarks
order
compare
between
embryos.
We
use
this
characterise
identities
within
caudal
lateral
epiblast
E8.5
revealing
local
emergence
early
mesoderm
cells
that
sensitive
inhibition
Notch
activity.
Developmental Cell,
Год журнала:
2022,
Номер
57(6), С. 707 - 718.e6
Опубликована: Март 1, 2022
Apoptosis
plays
an
important
role
in
morphogenesis,
and
the
notion
that
apoptotic
cells
can
impact
their
surroundings
came
to
light
recently.
However,
how
this
applies
vertebrate
morphogenesis
remains
unknown.
Here,
we
use
formation
of
neural
tube
determine
apoptosis
contributes
vertebrates.
Neural
closure
defects
have
been
reported
when
is
impaired
vertebrates,
although
cellular
mechanisms
involved
are
Using
avian
embryos,
found
generate
apico-basal
force
before
being
extruded
from
neuro-epithelium.
This
force,
which
relies
on
a
contractile
actomyosin
cable
extends
along
axis
cell,
drives
nuclear
fragmentation
influences
neighboring
tissue.
Together
with
morphological
observed
prevented,
these
data
strongly
suggest
neuroepithelium
keeps
track
mechanical
forces,
cumulatively,
contribute
actively
bending.
International Journal of Molecular Sciences,
Год журнала:
2022,
Номер
23(2), С. 845 - 845
Опубликована: Янв. 13, 2022
The
meso-diencephalic
dopaminergic
(mdDA)
neurons
regulate
various
critical
processes
in
the
mammalian
nervous
system,
including
voluntary
movement
and
a
wide
range
of
behaviors
such
as
mood,
reward,
addiction,
stress.
mdDA
neuronal
loss
is
linked
with
one
most
prominent
human
neurological
disorders,
Parkinson's
disease
(PD).
How
these
cells
die
regenerate
are
two
hotly
debated
PD
research
topics.
As
for
latter,
it
has
been
long
known
that
series
transcription
factors
(TFs)
involves
development
neurons,
specifying
cell
types
controlling
developmental
patterns.
In
vitro
vivo,
TFs
expression
tyrosine
hydroxylase,
dopamine
transporter,
vesicular
monoamine
transporter
2,
L-aromatic
amino
acid
decarboxylase,
all
which
synthesis
transport
(DA
neurons).
this
review,
we
encapsulate
molecular
mechanism
underlying
embryonic
growth
maturation
update
achievements
on
therapy
dependent
knowledge
development.
We
believe
deeper
understanding
extrinsic
intrinsic
influence
DA
neurons'
fate
midbrain
could
lead
to
better
strategy
therapy.
Physics Reports,
Год журнала:
2024,
Номер
1080, С. 1 - 104
Опубликована: Май 27, 2024
Proper
vertebrae
formation
relies
on
a
tissue-wide
oscillator
called
the
segmentation
clock.
Individual
cellular
oscillators
in
presomitic
mesoderm
are
modulated
by
intercellular
coupling
and
external
signals,
leading
to
propagation
of
oscillatory
waves
genetic
expression
eventually
stabilizing
into
static
pattern.
Here,
we
review
4
decades
biophysical
models
this
process,
starting
from
pioneering
Clock
Wavefront
model
Cooke
Zeeman,
reaction–diffusion
Meinhardt.
We
discuss
how
modern
descriptions
followed
advances
molecular
description
visualization
reviewing
phase
models,
delayed
systems-level,
finally
geometric
models.
connect
high-level
aspects
embryonic
development
scaling
wave
propagation,
up
reconstructed
stem
cell
systems.
provide
new
analytical
calculations
insights
classical
recent
us
propose
somitogenesis
organized
along
two
primary
differentiation.
Frontiers in Cellular Neuroscience,
Год журнала:
2021,
Номер
15
Опубликована: Апрель 26, 2021
The
ability
to
reliably
repair
spinal
cord
injuries
(SCI)
will
be
one
of
the
greatest
human
achievements
realized
in
regenerative
medicine.
Until
recently,
cellular
path
this
goal
has
been
challenging.
However,
as
detailed
developmental
principles
are
revealed
mouse
and
models,
their
application
stem
cell
community
brings
trunk
spine
embryology
into
efforts
advance
New
models
posterior
embryo
development
identify
neuromesodermal
progenitors
(NMPs)
a
major
bifurcation
point
generating
somites
is
leading
production
types
with
full
range
axial
identities
critical
for
disorders.
This
coupled
organoid
technologies
including
assembloids,
circuitoids,
gastruloids.
We
describe
paradigm
applying
towards
cell-based
restorative
therapies
enable
reproducible
effective
near-term
clinical
interventions.
iScience,
Год журнала:
2022,
Номер
25(10), С. 105216 - 105216
Опубликована: Сен. 26, 2022
The
transition
state
model
of
cell
differentiation
proposes
that
a
transient
window
gene
expression
stochasticity
precedes
entry
into
differentiated
state.
Here,
we
assess
this
theoretical
in
zebrafish
neuromesodermal
progenitors
(NMps)
vivo
during
late
somitogenesis
stages.
We
observed
an
increase
variability
at
the
24
somite
stage
(24ss)
before
their
spinal
cord
and
paraxial
mesoderm.
Analysis
published
18ss
scRNA-seq
dataset
showed
NMp
population
is
noisier
than
its
derivatives.
By
building
silico
composite
maps
from
image
data,
assigned
'NM
index'
to
NMps
based
on
neural
mesodermal
markers
demonstrated
heterogeneity
peaked
24ss.
Further
examination
revealed
cells
with
profiles
incongruent
prospective
fate.
Taken
together,
our
work
supports
within
endogenous
fate
decision
making
event.
A
major
challenge
in
biology
is
to
understand
how
mechanical
interactions
and
cellular
behavior
affect
the
shapes
of
tissues
embryo
morphology.
The
extension
neural
tube
paraxial
mesoderm,
which
form
spinal
cord
musculoskeletal
system,
respectively,
results
elongated
shape
vertebrate
embryonic
body.
Despite
our
understanding
each
these
elongates
independently
others,
morphogenetic
consequences
their
simultaneous
growth
are
still
unclear.
Our
study
investigates
differential
growth,
tissue
biophysical
properties
morphogenesis
during
axial
using
a
2D
multi-tissue
continuum-based
mathematical
model.
model
captures
dynamics
observed
vivo
by
time-lapse
imaging
bird
embryos,
reveals
underestimated
influence
proliferation
rates.
We
confirmed
this
prediction
quail
embryos
showing
that
decreasing
rate
cell
mesoderm
affects
long-term
dynamics,
shaping
both
neighboring
tube.
Overall,
work
provides
new
theoretical
platform
upon
consider
on
morphogenesis.
