Rev-Rev Response Element Activity Selection Bias at the HIV Transmission Bottleneck DOI Creative Commons
Patrick E. H. Jackson, Jordan Holsey, Lauren Turse

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Апрель 5, 2023

Abstract HIV is not efficiently transmitted between hosts, and selection of viral variants occurs during the process sexual transmission. The factors that confer selective advantage at transmission bottleneck remain incompletely understood. We explored whether differences in Rev-Rev Response Element (RRE) regulatory axis affect fitness, since functional variation Rev-RRE different isolates has been shown to replication kinetics relative expression many proteins. Single genome sequences were identified from nine linked subject pairs near time female-to-male Using a rapid flow-cytometric assay, we found activity varied significantly isolates. Moreover, it was generally lower recipients’ viruses compared corresponding donor viruses. In six events, recipient virus clustered extreme low end range activity. pair an unpredictable product component Rev RRE variation. These data indicate pressure on Variation may permit adaptation fitness landscapes could play important role pathogenesis.

Язык: Английский

Rev-Rev Response Element Activity Selection Bias at the HIV Transmission Bottleneck DOI Creative Commons
Patrick E. H. Jackson, Jordan Holsey, Lauren Turse

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Апрель 5, 2023

Abstract HIV is not efficiently transmitted between hosts, and selection of viral variants occurs during the process sexual transmission. The factors that confer selective advantage at transmission bottleneck remain incompletely understood. We explored whether differences in Rev-Rev Response Element (RRE) regulatory axis affect fitness, since functional variation Rev-RRE different isolates has been shown to replication kinetics relative expression many proteins. Single genome sequences were identified from nine linked subject pairs near time female-to-male Using a rapid flow-cytometric assay, we found activity varied significantly isolates. Moreover, it was generally lower recipients’ viruses compared corresponding donor viruses. In six events, recipient virus clustered extreme low end range activity. pair an unpredictable product component Rev RRE variation. These data indicate pressure on Variation may permit adaptation fitness landscapes could play important role pathogenesis.

Язык: Английский

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