Confronting the loss of trophic support DOI Creative Commons

Hui‐Lan Hu,

Latika Khatri,

Marilyn Santacruz

и другие.

Frontiers in Molecular Neuroscience, Год журнала: 2023, Номер 16

Опубликована: Июнь 23, 2023

Classic experiments with peripheral sympathetic neurons established an absolute dependence upon NGF for survival. A forgotten problem is how these become resistant to deprivation of trophic factors. The question whether and can survive in the absence support. However, mechanism not understood switch their phenotype lose on factors, such as BDNF. Here, we approach by considering requirements support hippocampal from central nervous system. We developed cellular assays assess factor dependency identified factors that rescue They include enhanced expression a subunit receptor (Neurotrophin Receptor Homolog, NRH) increase glucocorticoid neurons. results are significant since levels activity responsible many neuropsychiatric conditions. Resistance may be relevant underlying basis longevity, well important element preventing neurodegeneration.

Язык: Английский

FM1-43 Dye Memorizes Piezo1 Activation in the Trigeminal Nociceptive System Implicated in Migraine Pain DOI Open Access
Adriana Della Pietra, Nikita Mikhailov, Rashid Giniatullin

и другие.

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(2), С. 1688 - 1688

Опубликована: Янв. 14, 2023

It has been proposed that mechanosensitive Piezo1 channels trigger migraine pain in trigeminal nociceptive neurons, but the mechanosensitivity of satellite glial cells (SGCs) supporting neuronal sensitization not tested before. Moreover, tools to monitor previous activation are available. Therefore, by using live calcium imaging with Fluo-4 AM and labeling FM1-43 dye, we explored a new strategy identify Piezo channels' activity mouse SGCs, isolated meninges. The specific agonist Yoda1 induced transients both neurons suggesting functional expression types cells. In Piezo1-transfected HEK cells, produced only transient fluorescent response, whereas co-application provided higher signals remarkable long-lasting 'tail response'. A similar Piezo1-related trapping was observed SGCs. non-specific channel blocker, Gadolinium, inhibited peak, confirming involvement receptors. Finally, demonstrated meningeal tissues 3.5 h after washout. Our data indicated SGCs express channels, their provides sustained FM1-43. This labelling can be used ongoing system, which is implicated pain.

Язык: Английский

Процитировано

8
Pannexin-1 opening in neuronal edema causes cell death but also leads to protection via increased microglia contacts DOI Creative Commons
Nicholas L. Weilinger, Kai Yang, Hyun B. Choi

и другие.

Cell Reports, Год журнала: 2023, Номер 42(10), С. 113128 - 113128

Опубликована: Сен. 23, 2023

Neuronal swelling during cytotoxic edema is triggered by Na+ and Cl− entry Ca2+ independent. However, the causes of neuronal death are unknown. Here, we investigate role large-conductance Pannexin-1 (Panx1) channels in edema. Panx1 channel inhibitors reduce delay voltage-gated with veratridine. downstream production reactive oxygen species (ROS) that opens channels. We confirm ROS activates currents whole-cell electrophysiology find scavenging neuroprotective. opening subsequent ATP release attract microglial processes to contact neurons. Depleting microglia using CSF1 receptor antagonist PLX3397 or blocking P2Y12 receptors exacerbates death, suggesting Panx1-ATP-dependent contacts conclude triggers oxidative stress neurons trigger but also initiates neuroprotective feedback mediated contacts.

Язык: Английский

Процитировано

7
Molecular characterization of nodose ganglia development reveals a novel population of Phox2b+ glial progenitors in mice DOI
Elijah D. Lowenstein,

Aristotelis Misios,

Sven Buchert

и другие.

Journal of Neuroscience, Год журнала: 2024, Номер 44(28), С. e1441232024 - e1441232024

Опубликована: Июнь 3, 2024

The vagal ganglia, comprised of the superior (jugular) and inferior (nodose) ganglia vagus nerve, receive somatosensory information from head neck or viscerosensory inner organs, respectively. Developmentally, cranial neural crest gives rise to all glial cells neurons jugular while epibranchial placode nodose ganglia. Crest-derived progenitors can additionally generate autonomic in peripheral nervous system, but how these is unknown. Here, we found that some Sox10+ crest-derived in, surrounding, ganglion transiently expressed Phox2b, a master regulator system development, during early embryonic life. Our genetic lineage-tracing analysis mice either sex revealed despite their common developmental origin extreme spatial proximity, substantial proportion nodose, not neighboring have history Phox2b expression. We used single-cell RNA-sequencing demonstrate give major subtypes including Schwann cells, satellite glia, precursors, mapped distribution by situ hybridization. Lastly, integration transcriptomic similarities between dorsal root immature subtypes. work demonstrates express entire complement display transcriptional program may underlie bipotent nature.

Язык: Английский

Процитировано

2
Hypertension increases sympathetic neuron activity by enhancing intraganglionic cholinergic collateral connections DOI
Ming‐Hua Li,

Michelle L. Sorensen,

Morgan Johnson

и другие.

The Journal of Physiology, Год журнала: 2024, Номер unknown

Опубликована: Июнь 21, 2024

Abstract Autonomic dysregulation, including sympathetic hyperactivity, is a common feature of hypertension (HT) and other cardiovascular diseases. The CNS plays role in driving chronic activation disease, but several lines evidence suggest that neuroplasticity the periphery may also contribute. potential contribution postganglionic neurons to sustained hyperactivity not well understood. We recently discovered noradrenergic stellate ganglion (SG) have excitatory cholinergic collateral connections within ganglion. hypothesize remodelling these increased transmission contributes diseases, HT. To test hypothesis, we examined activity isolated SG under control conditions after 1 week HT induced by peripheral angiotensin II infusion, using whole‐cell patch clamp recordings. Despite absence central inputs, observed elevated spontaneous synaptic from hypertensive mice required generation action potentials. Genetically disrupting decreased basal neuronal prevented II‐mediated enhancement activity. Similar changes activity, driven transmission, were identified cardiac projecting brown adipose tissue. These altered A‐type K + currents. This suggests stimulates throughout intraganglionic network connections, contributing characteristic disease. image Key points Sympathetic ganglia II‐treated are more active than despite activation. enhanced result ganglionic collaterals, rather intrinsic excitability. Increased was both tissue‐projecting neurons, which involved homeostasis.

Язык: Английский

Процитировано

2
Satellite glial cell manipulation prior to axotomy enhances developing dorsal root ganglion central branch regrowth into the spinal cord DOI Creative Commons
R. Brown, Heather M. Barber, Sarah Kucenas

и другие.

Glia, Год журнала: 2024, Номер 72(10), С. 1766 - 1784

Опубликована: Июнь 22, 2024

The central and peripheral nervous systems (CNS PNS, respectively) exhibit remarkable diversity in the capacity to regenerate following neuronal injury with PNS injuries being much more likely than those that occur CNS. Glial responses damage greatly influence likelihood of regeneration by either promoting or inhibiting axonal regrowth over time. However, despite our understanding how some glial lineages participate nerve degeneration regeneration, less is known about contributions satellite cells (SGC) failure axon branch dorsal root ganglia (DRG) sensory neurons. Here, using vivo, time-lapse imaging larval zebrafish coupled laser axotomy, we investigate role SGCs regeneration. In studies show respond relocating their nuclei site during same period DRG neurons produce new neurites. Laser ablation prior results neurite growth attempts ultimately a higher rate successful regrowth, implicating as inhibitors We also demonstrate this SGC response mediated part ErbB signaling, chemical inhibition receptor reduced motility enhanced regrowth. These findings provide insights into SGC-neuron interactions under conditions these system repair.

Язык: Английский

Процитировано

2
Mind the GAPS: Glia associated with psychological stress DOI Creative Commons
Niklas Blank, Molly Weiner, Shaan Patel

и другие.

Journal of Neuroendocrinology, Год журнала: 2024, Номер unknown

Опубликована: Окт. 9, 2024

Glial cells are an integral component of the nervous system, performing crucial functions that extend beyond structural support, including modulation immune tissue repair, and maintaining homeostasis. Recent studies have highlighted importance glial as key mediators stress responses across different organs. This review focuses on roles in peripheral tissues health their involvement diseases linked to psychological stress. Populations glia associated with ("GAPS") emerge a promising target cell population our basic understanding stress-associated pathologies, highlighting role deleterious effects various conditions. Ultimately, new insights into impact populations periphery may support clinical efforts aimed at improving state patients for improved outcomes.

Язык: Английский

Процитировано

1
Store-operated calcium entry in the satellite glial cells of rat sympathetic ganglia DOI Open Access
Sohyun Kim, Seong Jun Kang, Huu Son Nguyen

и другие.

Korean Journal of Physiology and Pharmacology, Год журнала: 2023, Номер 28(1), С. 93 - 103

Опубликована: Дек. 29, 2023

Satellite glial cells (SGCs), a major type of cell in the autonomic ganglia, closely envelop body and even synaptic regions single neuron with very narrow gap. This structurally unique organization suggests that neurons SGCs may communicate reciprocally. Glial Ca

Язык: Английский

Процитировано

3
Heightened sympathetic neuron activity and altered cardiomyocyte properties in spontaneously hypertensive rats during the postnatal period DOI Creative Commons
Marián Haburčák, Joshua G. Harrison,

Melda M. Buyukozturk

и другие.

Frontiers in Synaptic Neuroscience, Год журнала: 2022, Номер 14

Опубликована: Сен. 30, 2022

The Spontaneously Hypertensive Rat (SHR) has increased sympathetic drive to the periphery that precedes and contributes development of high blood pressure, making it a useful model for study neurogenic hypertension. Comparisons normotensive Wistar Kyoto (WKY) rat have demonstrated altered active intrinsic properties SHR neurons shortly before onset Here we examine structural functional plasticity postnatal WKY cultured alone or co-cultured with cardiomyocytes under conditions limited extrinsic signaling. an number synaptic sites compared age-matched neurons, measured by co-localization presynaptic vesicular acetylcholine transporter postsynaptic shank proteins. Whole cell recordings show higher charge than demonstrating increase in is associated transmission. Differences are not firing rates between influenced interactions target from either strain. Both tonic patterns our cultures, which depleted non-neuronal ganglionic cells provide neurotrophic This suggests normal mature, phasic requires signaling, potentially differential responses prehypertensive SHR, been reported maintain at later developmental stages. While do neuronal differences display decreased hypertrophy neurons. These experiments suggest signaling changes cardiac-sympathetic circuit rats as early period.

Язык: Английский

Процитировано

4
Sympathetic NPY controls glucose homeostasis, cold tolerance, and cardiovascular functions in mice DOI Creative Commons
Raniki Kumari, Raluca Paşcalău, Hui Wang

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Июль 26, 2023

Neuropeptide Y (NPY) is best known for its effects in the brain as an orexigenic and anxiolytic agent reducing energy expenditure. NPY also co-expressed with Norepinephrine (NE) sympathetic neurons. Although generally considered to modulate noradrenergic responses, specific roles autonomic physiology remain under-appreciated. Here, we show that sympathetic-derived essential metabolic cardiovascular regulation mice. NE are 90% of prevertebral neurons only 43% paravertebral NPY-expressing primarily innervate blood vessels peripheral organs. Sympathetic-specific deletion elicits pronounced defects mice, including reductions insulin secretion, glucose tolerance, cold pupil size, elevation heart rate, while notably, however, basal pressure was unchanged. These findings provide new knowledge about target tissue-specific functions derived from imply potential involvement diseases.

Язык: Английский

Процитировано

1
The role of glial cells in amyotrophic lateral sclerosis DOI

Virenkumar A. Pandya,

Rickie Patani

International review of neurobiology, Год журнала: 2024, Номер unknown, С. 381 - 450

Опубликована: Янв. 1, 2024

Язык: Английский

Процитировано

0