Genetic variation in IL-4 activated tissue resident macrophages determines strain-specific synergistic responses to LPS epigenetically DOI Creative Commons
mingming zhao, Dragana Janković, Verena M. Link

и другие.

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 25, 2025

Abstract How macrophages in the tissue environment integrate multiple stimuli depends on genetic background of host, but this is still poorly understood. We investigate IL-4 activation male C57BL/6 and BALB/c strain specific vivo tissue-resident (TRMs) from peritoneal cavity. TRMs are more transcriptionally responsive to stimulation, with induced genes associated super enhancers, topologically associating domains (TAD) boundaries. IL-4-directed epigenomic remodeling reveals enrichment NF-κB, IRF, STAT motifs. Additionally, IL-4-activated demonstrate an augmented synergistic response upon vitro lipopolysaccharide (LPS) exposure, despite naïve displaying a robust transcriptional LPS. Single-cell RNA sequencing (scRNA-seq) analysis mixed bone marrow chimeras indicates that differences synergy cell intrinsic within same environment. Hence, variation alters IL-4-induced epigenetic reprogramming resulting responses LPS exposure.

Язык: Английский

Biomaterials that passively and actively target macrophages promote the regeneration of injured tissues DOI Creative Commons

Pengzhen Zhuang,

Yang Wu, Yu Chen

и другие.

Biomedical Technology, Год журнала: 2024, Номер 8, С. 17 - 49

Опубликована: Окт. 1, 2024

Язык: Английский

Процитировано

4
Glycolytic reprogramming in microglia: A potential therapeutic target for ischemic stroke DOI
Guangming Zhang,

Anliu Zhao,

Xiaolu Zhang

и другие.

Cellular Signalling, Год журнала: 2024, Номер unknown, С. 111466 - 111466

Опубликована: Окт. 1, 2024

Язык: Английский

Процитировано

4
Herbacetin ameliorates lipopolysaccharide-elicited inflammatory response by suppressing NLRP-3/AIM-2 inflammasome activation, PI3K/Akt/MAPKs/NF-κB redox inflammatory signalling, modulating autophagy and macrophage polarization imbalance DOI
Monika Kumari, Anamika Sharma, Narendra Vijay Tirpude

и другие.

Molecular Biology Reports, Год журнала: 2024, Номер 51(1)

Опубликована: Ноя. 16, 2024

Язык: Английский

Процитировано

3
Identification of glutamine as a potential therapeutic target in dry eye disease DOI Creative Commons
Xiaoniao Chen, Chuyue Zhang, Fei Peng

и другие.

Signal Transduction and Targeted Therapy, Год журнала: 2025, Номер 10(1)

Опубликована: Янв. 21, 2025

Abstract Dry eye disease (DED) is a prevalent inflammatory condition significantly impacting quality of life, yet lacks effective pharmacological therapies. Herein, we proposed novel approach to modulate the inflammation through metabolic remodeling, thus promoting dry recovery. Our study demonstrated that co-treatment with mesenchymal stem cells (MSCs) and thymosin beta-4 (Tβ4) yielded best therapeutic outcome against eye, surpassing monotherapy outcomes. In situ metabolomics matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) revealed increased glutamine levels in cornea following MSC + Tβ4 combined therapy. Inhibition reversed anti-inflammatory, anti-apoptotic, homeostasis-preserving effects observed therapy, highlighting critical role Clinical cases rodent model showed elevated expression glutaminase (GLS1), an upstream enzyme metabolism, injury. Mechanistic studies indicated overexpression inhibition GLS1 counteracted enhanced, respectively, anti-inflammatory underscoring GLS1’s pivotal regulating metabolism. Furthermore, single-cell sequencing distinct subset pro-inflammatory pro-fibrotic corneal epithelial model, while treatment downregulated those subclusters, thereby reducing their cytokine secretion. summary, effectively ameliorated occurrence apoptosis by downregulating subclusters related IκBα/NF-κB signaling. The present suggests metabolism plays critical, previously unrecognized DED proposes attractive strategy enhance inhibiting alleviating inflammation-driven progression.

Язык: Английский

Процитировано

0
Genetic variation in IL-4 activated tissue resident macrophages determines strain-specific synergistic responses to LPS epigenetically DOI Creative Commons
mingming zhao, Dragana Janković, Verena M. Link

и другие.

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 25, 2025

Abstract How macrophages in the tissue environment integrate multiple stimuli depends on genetic background of host, but this is still poorly understood. We investigate IL-4 activation male C57BL/6 and BALB/c strain specific vivo tissue-resident (TRMs) from peritoneal cavity. TRMs are more transcriptionally responsive to stimulation, with induced genes associated super enhancers, topologically associating domains (TAD) boundaries. IL-4-directed epigenomic remodeling reveals enrichment NF-κB, IRF, STAT motifs. Additionally, IL-4-activated demonstrate an augmented synergistic response upon vitro lipopolysaccharide (LPS) exposure, despite naïve displaying a robust transcriptional LPS. Single-cell RNA sequencing (scRNA-seq) analysis mixed bone marrow chimeras indicates that differences synergy cell intrinsic within same environment. Hence, variation alters IL-4-induced epigenetic reprogramming resulting responses LPS exposure.

Язык: Английский

Процитировано

0