Hsf1 and the molecular chaperone Hsp90 support a “rewiring stress response” leading to an adaptive cell size increase in chronic stress DOI Creative Commons
Samarpan Maiti, Kaushik Bhattacharya, Diana Wider

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Янв. 25, 2023

Abstract Cells are exposed to a wide variety of internal and external stresses. Whereas many studies have focused on cellular responses acute severe stresses, little is known about how systems adapt sublethal chronic Using mammalian cells in culture, we discovered that they mild stresses up two weeks, notably proteotoxic such as heat, by increasing their size translation, thereby scaling the amount total protein. These adaptations render them more resilient persistent subsequent We demonstrate Hsf1, well for its role stress responses, required cell increase, molecular chaperone Hsp90 essential coupling increase augmented translation. term this translational reprogramming “rewiring response”, propose protective process adaptation contributes get older, failure promotes aging.

Язык: Английский

Influence of the Age of Free-Living Amoeba Cysts on Their Vertical Distribution in a Water Column DOI Creative Commons
Zineb Fechtali‐Moute, Sébastien Pomel

Microorganisms, Год журнала: 2024, Номер 12(3), С. 474 - 474

Опубликована: Фев. 27, 2024

Free-living amoebae (FLA) are widely distributed protozoa in both natural and artificial environments such as drinking water. In addition to the ability of all FLA transport various pathogenic microorganisms, certain species, Acanthamoeba spp. or Balamuthia mandrillaris, have intrinsic abilities cause severe cerebral infections. Previous work has shown an enrichment cysts biofilm developed upper levels Drinking Water Storage Towers (DWSTs), suggesting that differences densities may play a role their unequal distribution water column. To evaluate this hypothesis, model column was created for study used analyze vertical castellanii, Vermamoeba vermiformis, mandrillaris from 0 23 weeks. Interestingly, our data showed A. castellanii V. vermiformis were enriched during aging. However, B. equally entire study. These results show that, level variation DWST, some can become less dense aging, which contributes therefore levels.

Язык: Английский

Процитировано

1
DHM/SERS reveals cellular morphology and molecular changes during iPSCs-derived activation of astrocytes DOI Creative Commons

Xiaoya Bu,

Liwei Yang,

Xianxin Han

и другие.

Biomedical Optics Express, Год журнала: 2024, Номер 15(6), С. 4010 - 4010

Опубликована: Май 24, 2024

The activation of astrocytes derived from induced pluripotent stem cells (iPSCs) is great significance in neuroscience research, and it crucial to obtain both cellular morphology biomolecular information non-destructively situ, which still complicated by the traditional optical microscopy biochemical methods such as immunofluorescence western blot. In this study, we combined digital holographic (DHM) surface-enhanced Raman scattering (SERS) investigate characteristics iPSCs-derived astrocytes. It was found that projected area activated decreased 67%, while cell dry mass increased 23%, changed a flat polygonal shape an elongated star-shaped morphology. SERS analysis further revealed increase intensities protein spectral peaks (phenylalanine 1001 cm -1 , proline 1043 etc.) lipid-related (phosphatidylserine 524 triglycerides 1264 intensity. Principal component analysis-linear discriminant (PCA-LDA) modeling based on data distinguished resting reactive with high accuracy 96.5%. correlated content, decrease indicated adjustment lipid composition membrane remodeling. Importantly, results not only reveal molecular changes during but also reflect their mapping relationship, thereby providing new insights into diagnosing treating neurodegenerative diseases.

Язык: Английский

Процитировано

1
Intracellular dry mass density increases under growth-induced pressure DOI Creative Commons
Hyo-Jun Kim,

Baptiste Alric,

Nolan Chan

и другие.

Open Research Europe, Год журнала: 2024, Номер 4, С. 231 - 231

Опубликована: Дек. 19, 2024

Cells that proliferate in confined environments develop mechanical compressive stress, referred to as growth-induced pressure, which inhibits growth and division across various organisms. Recent studies have shown these spaces, the diffusivity of intracellular nanoparticles decreases. However, physical mechanisms behind this reduction remain unclear. In study, we use quantitative phase imaging measure refractive index dry mass density Saccharomyces cerevisiae cells proliferating under confinement a microfluidic bioreactor. Our results indicate observed decrease could be attributed accumulation macromolecules. Furthermore, linear scaling between cell content pressure suggests concentrations macromolecules osmolytes are maintained proportionally such S. cerevisiae.

Язык: Английский

Процитировано

1
Cyanobacteria newly isolated from marine volcanic seeps display rapid sinking and robust, high-density growth DOI Creative Commons
Max G. Schubert, Tzu‐Chieh Tang, Isabella M. Goodchild-Michelman

и другие.

Applied and Environmental Microbiology, Год журнала: 2024, Номер 90(11)

Опубликована: Окт. 29, 2024

ABSTRACT Cyanobacteria are photosynthetic organisms that play important roles in carbon cycling and promising bioproduction chassis. Here, we isolate two novel cyanobacteria with 4.6Mbp genomes, UTEX 3221 3222, from a unique marine environment naturally elevated CO₂. We describe complete genome sequences for both isolates and, focusing on 3222 due to its planktonic growth liquid, characterize biotechnologically relevant biomass characteristics. outpaces other fast-growing model strains solid medium. It can double every 2.35 hours liquid medium grows high density (>31 g/L dry weight) batch culture, nearly of Synechococcus sp. PCC 11901, whose high-density was recently reported. In addition, sinks readily, settling more quickly than strains, suggesting favorable economics harvesting biomass. These traits may make compelling choice dioxide removal (CDR) Overall, find bio-prospecting environments CO₂ uncover CO₂-metabolizing IMPORTANCE provide potential avenue biomanufacturing combatting climate change via high-efficiency sequestration. This study identifies isolated geochemical describes their behavior biochemical composition. appear tractable research model, cultures made publicly available alongside information about culture maintenance. Application these sequestration and/or is discussed, including unusual, rapid characteristics the scaled culture.

Язык: Английский

Процитировано

1
Hsf1 and the molecular chaperone Hsp90 support a “rewiring stress response” leading to an adaptive cell size increase in chronic stress DOI Creative Commons
Samarpan Maiti, Kaushik Bhattacharya, Diana Wider

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Янв. 25, 2023

Abstract Cells are exposed to a wide variety of internal and external stresses. Whereas many studies have focused on cellular responses acute severe stresses, little is known about how systems adapt sublethal chronic Using mammalian cells in culture, we discovered that they mild stresses up two weeks, notably proteotoxic such as heat, by increasing their size translation, thereby scaling the amount total protein. These adaptations render them more resilient persistent subsequent We demonstrate Hsf1, well for its role stress responses, required cell increase, molecular chaperone Hsp90 essential coupling increase augmented translation. term this translational reprogramming “rewiring response”, propose protective process adaptation contributes get older, failure promotes aging.

Язык: Английский

Процитировано

1