bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 4, 2024
ABSTRACT
All
cells
are
subject
to
geometric
constraints,
such
as
surface
area-to-volume
(SA/V)
ratio,
that
impact
cell
functions
and
force
biological
adaptations.
Like
the
SA/V
ratio
of
a
sphere,
it
is
generally
assumed
decreases
size
increases.
Here,
we
investigate
this
in
near-spherical
mammalian
using
single-cell
measurements
mass
proteins,
well
imaging
plasma
membrane
morphology.
We
find
remains
surprisingly
constant
grow
larger.
This
observation
largely
independent
cycle
amount
growth.
Consequently,
growth
results
increased
folding,
which
simplifies
cellular
design
by
ensuring
sufficient
area
for
division,
nutrient
uptake
deformation
at
all
sizes.
Microorganisms,
Год журнала:
2024,
Номер
12(3), С. 474 - 474
Опубликована: Фев. 27, 2024
Free-living
amoebae
(FLA)
are
widely
distributed
protozoa
in
both
natural
and
artificial
environments
such
as
drinking
water.
In
addition
to
the
ability
of
all
FLA
transport
various
pathogenic
microorganisms,
certain
species,
Acanthamoeba
spp.
or
Balamuthia
mandrillaris,
have
intrinsic
abilities
cause
severe
cerebral
infections.
Previous
work
has
shown
an
enrichment
cysts
biofilm
developed
upper
levels
Drinking
Water
Storage
Towers
(DWSTs),
suggesting
that
differences
densities
may
play
a
role
their
unequal
distribution
water
column.
To
evaluate
this
hypothesis,
model
column
was
created
for
study
used
analyze
vertical
castellanii,
Vermamoeba
vermiformis,
mandrillaris
from
0
23
weeks.
Interestingly,
our
data
showed
A.
castellanii
V.
vermiformis
were
enriched
during
aging.
However,
B.
equally
entire
study.
These
results
show
that,
level
variation
DWST,
some
can
become
less
dense
aging,
which
contributes
therefore
levels.
Biomedical Optics Express,
Год журнала:
2024,
Номер
15(6), С. 4010 - 4010
Опубликована: Май 24, 2024
The
activation
of
astrocytes
derived
from
induced
pluripotent
stem
cells
(iPSCs)
is
great
significance
in
neuroscience
research,
and
it
crucial
to
obtain
both
cellular
morphology
biomolecular
information
non-destructively
situ,
which
still
complicated
by
the
traditional
optical
microscopy
biochemical
methods
such
as
immunofluorescence
western
blot.
In
this
study,
we
combined
digital
holographic
(DHM)
surface-enhanced
Raman
scattering
(SERS)
investigate
characteristics
iPSCs-derived
astrocytes.
It
was
found
that
projected
area
activated
decreased
67%,
while
cell
dry
mass
increased
23%,
changed
a
flat
polygonal
shape
an
elongated
star-shaped
morphology.
SERS
analysis
further
revealed
increase
intensities
protein
spectral
peaks
(phenylalanine
1001
cm
-1
,
proline
1043
etc.)
lipid-related
(phosphatidylserine
524
triglycerides
1264
intensity.
Principal
component
analysis-linear
discriminant
(PCA-LDA)
modeling
based
on
data
distinguished
resting
reactive
with
high
accuracy
96.5%.
correlated
content,
decrease
indicated
adjustment
lipid
composition
membrane
remodeling.
Importantly,
results
not
only
reveal
molecular
changes
during
but
also
reflect
their
mapping
relationship,
thereby
providing
new
insights
into
diagnosing
treating
neurodegenerative
diseases.
Applied and Environmental Microbiology,
Год журнала:
2024,
Номер
90(11)
Опубликована: Окт. 29, 2024
ABSTRACT
Cyanobacteria
are
photosynthetic
organisms
that
play
important
roles
in
carbon
cycling
and
promising
bioproduction
chassis.
Here,
we
isolate
two
novel
cyanobacteria
with
4.6Mbp
genomes,
UTEX
3221
3222,
from
a
unique
marine
environment
naturally
elevated
CO₂.
We
describe
complete
genome
sequences
for
both
isolates
and,
focusing
on
3222
due
to
its
planktonic
growth
liquid,
characterize
biotechnologically
relevant
biomass
characteristics.
outpaces
other
fast-growing
model
strains
solid
medium.
It
can
double
every
2.35
hours
liquid
medium
grows
high
density
(>31
g/L
dry
weight)
batch
culture,
nearly
of
Synechococcus
sp.
PCC
11901,
whose
high-density
was
recently
reported.
In
addition,
sinks
readily,
settling
more
quickly
than
strains,
suggesting
favorable
economics
harvesting
biomass.
These
traits
may
make
compelling
choice
dioxide
removal
(CDR)
Overall,
find
bio-prospecting
environments
CO₂
uncover
CO₂-metabolizing
IMPORTANCE
provide
potential
avenue
biomanufacturing
combatting
climate
change
via
high-efficiency
sequestration.
This
study
identifies
isolated
geochemical
describes
their
behavior
biochemical
composition.
appear
tractable
research
model,
cultures
made
publicly
available
alongside
information
about
culture
maintenance.
Application
these
sequestration
and/or
is
discussed,
including
unusual,
rapid
characteristics
the
scaled
culture.
Open Research Europe,
Год журнала:
2024,
Номер
4, С. 231 - 231
Опубликована: Дек. 19, 2024
Cells
that
proliferate
in
confined
environments
develop
mechanical
compressive
stress,
referred
to
as
growth-induced
pressure,
which
inhibits
growth
and
division
across
various
organisms.
Recent
studies
have
shown
these
spaces,
the
diffusivity
of
intracellular
nanoparticles
decreases.
However,
physical
mechanisms
behind
this
reduction
remain
unclear.
In
study,
we
use
quantitative
phase
imaging
measure
refractive
index
dry
mass
density
Saccharomyces
cerevisiae
cells
proliferating
under
confinement
a
microfluidic
bioreactor.
Our
results
indicate
observed
decrease
could
be
attributed
accumulation
macromolecules.
Furthermore,
linear
scaling
between
cell
content
pressure
suggests
concentrations
macromolecules
osmolytes
are
maintained
proportionally
such
S.
cerevisiae.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 4, 2024
ABSTRACT
All
cells
are
subject
to
geometric
constraints,
such
as
surface
area-to-volume
(SA/V)
ratio,
that
impact
cell
functions
and
force
biological
adaptations.
Like
the
SA/V
ratio
of
a
sphere,
it
is
generally
assumed
decreases
size
increases.
Here,
we
investigate
this
in
near-spherical
mammalian
using
single-cell
measurements
mass
proteins,
well
imaging
plasma
membrane
morphology.
We
find
remains
surprisingly
constant
grow
larger.
This
observation
largely
independent
cycle
amount
growth.
Consequently,
growth
results
increased
folding,
which
simplifies
cellular
design
by
ensuring
sufficient
area
for
division,
nutrient
uptake
deformation
at
all
sizes.