Olfactory receptor neurons generate multiple response motifs, increasing coding space dimensionality

eLife, Год журнала: 2023, Номер 12

Опубликована: Янв. 31, 2023

Odorants binding to olfactory receptor neurons (ORNs) trigger bursts of action potentials, providing the brain with its only experience environment. Our recordings made in vivo from locust ORNs showed that odor-elicited firing patterns comprise four distinct response motifs, each defined by a reliable temporal profile. Different odorants could elicit different motifs given ORN, property we term motif switching. Further, undergoes own form sensory adaptation when activated repeated plume-like odor pulses. A computational model constrained our revealed organizing responses into multiple provides substantial benefits for classifying odors and processing complex plumes: contributes uniquely encode plume’s composition structure. Multiple switching further improve classification expanding coding dimensionality. demonstrated these features provide navigation, including determining distance an source.

Язык: Английский

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Analysis of fast calcium dynamics of honey bee olfactory coding

eLife, Год журнала: 2024, Номер 13

Опубликована: Сен. 5, 2024

Odour processing exhibits multiple parallels between vertebrate and invertebrate olfactory systems. Insects, in particular, have emerged as relevant models for studies because of the tractability their circuits. Here, we used fast calcium imaging to track activity projection neurons honey bee antennal lobe (AL) during stimulation at high temporal resolution. We observed a heterogeneity response profiles an abundance inhibitory activities, resulting various latencies stimulus-specific post-odour neural signatures. Recorded signals were fed mushroom body (MB) model constructed implementing fundamental features connectivity neurons, Kenyon cells (KC), MB output (MBON). The accounts increase odorant discrimination compared AL reveals recruitment two distinct KC populations that represent odorants aftersmell separate but temporally coherent objects. Finally, showed learning-induced modulation KC-to-MBON synapses can explain both variations associative learning scores across different conditioning protocols bees bees' latency. Thus, it provides simple explanation how time contingency stimulus reward be encoded without need tracking. This study broadens our understanding coding bees. It demonstrates based on rules with real physiological data aspects odour learning.

Язык: Английский

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Analysis of fast calcium dynamics of honey bee olfactory coding

eLife, Год журнала: 2024, Номер 13

Опубликована: Фев. 15, 2024

Odour processing exhibits multiple parallels between vertebrate and invertebrate olfactory systems. Insects, in particular, have emerged as relevant models for studies because of the tractability their circuits. Here, we used fast calcium imaging to track activity projection neurons honey bee antennal lobe (AL) during stimulation at high temporal resolution. We observed a heterogeneity response profiles an abundance inhibitory activities, resulting various latencies stimulus-specific post-odour neural signatures. Recorded signals were fed mushroom body (MB) model constructed implementing fundamental features connectivity neurons, Kenyon cells (KC), MB output (MBON). The accounts increase odorant discrimination compared AL reveals recruitment two distinct KC populations that represent odorants aftersmell separate but temporally coherent objects. Finally, showed learning-induced modulation KC-to-MBON synapses can explain both variations associative learning scores across different conditioning protocols bees bees' latency. Thus, it provides simple explanation how time contingency stimulus reward be encoded without need tracking. This study broadens our understanding coding bees. It demonstrates based on rules with real physiological data aspects odour learning.

Язык: Английский

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Ring-shaped odor coding in the antennal lobe of migratory locusts

Cell, Год журнала: 2024, Номер 187(15), С. 3973 - 3991.e24

Опубликована: Июнь 18, 2024

The representation of odors in the locust antennal lobe with its >2,000 glomeruli has long remained a perplexing puzzle. We employed CRISPR-Cas9 system to generate transgenic locusts expressing genetically encoded calcium indicator GCaMP olfactory sensory neurons. Using two-photon functional imaging, we mapped spatial activation patterns representing wide range ecologically relevant across all six developmental stages. Our findings reveal functionally ring-shaped organization composed specific glomerular clusters. This configuration establishes an odor-specific chemotopic by encoding different chemical classes and distinct form rings. arrangement, which confirm selective targeting OR70a-expressing neurons, occurs throughout development, odor-coding pattern within population is consistent Mechanistically, this unconventional code reflects locust-specific multiplexed innervation lobe.

Язык: Английский

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Innate attraction and aversion to odors in locusts

PLoS ONE, Год журнала: 2023, Номер 18(7), С. e0284641 - e0284641

Опубликована: Июль 10, 2023

Many animals display innate preferences for some odors, but the physiological mechanisms underlying these are poorly understood. Here, with behavioral tests, we establish a model system well suited to investigating olfactory mechanisms, locust Schistocerca americana. We conducted open field tests in an arena designed provide only cues guide navigation choices. found that newly hatched locusts navigated toward, and spent more time near, odor of wheat grass than humidified air. In similar hatchlings avoided moderate concentrations major individual components food blend odor, 1-hexanol (1% v/v) hexanal (0.9% diluted mineral oil relative control presentations unscented oil. Hatchlings were neither attracted nor repelled by lower concentration (0.1% moderately low (0.225% hexanal. quantified behavior tracking their positions Argos software toolkit. Our results have strong, preference blend, valence blend's may be different change depending on concentration. useful entry point analysis sensory preferences.

Язык: Английский

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Olfactory system structure and function in newly hatched and adult locusts

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Ноя. 27, 2023

Abstract An important question in neuroscience is how sensory systems change as animals grow and interact with the environment. Exploring they develop can reveal networks of neurons process information themselves needs animal change. Here we compared structure function peripheral parts olfactory pathway newly hatched adult locusts. We found that populations (OSNs) hatchlings adults responded similar tunings to a panel odors. The morphologies local (LNs) projection (PNs) antennal lobes (ALs) were very both age groups, though smaller hatchlings, proportional overall brain size. odor evoked responses LNs PNs also characterized by complex patterns activity including oscillatory synchronization. Notably, spontaneous odor-evoked firing rates lower, LFP oscillations lower frequency, than adult. Hatchlings have antennae fewer OSNs; removing segments from reduced oscillation frequency. Thus, consistent earlier computational models, developmental increase frequency due increasing intensity input circuitry. Overall, our results show locusts hatch fully formed system structurally functionally matches adult, despite its small size lack prior experience stimuli.

Язык: Английский

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Olfactory system structure and function in newly hatched and adult locusts

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Янв. 31, 2024

Abstract An important question in neuroscience is how sensory systems change as animals grow and interact with the environment. Exploring they develop can reveal networks of neurons process information themselves needs animal change. Here we compared structure function peripheral parts olfactory pathway newly hatched adult locusts. We found that populations (OSNs) hatchlings adults responded similar tunings to a panel odors. The morphologies local (LNs) projection (PNs) antennal lobes (ALs) were very both age groups, though smaller hatchlings, proportional overall brain size. odor evoked responses LNs PNs also characterized by complex patterns activity including oscillatory synchronization. Notably, spontaneous odor-evoked firing rates lower, LFP oscillations lower frequency, than adult. Hatchlings have antennae fewer OSNs; removing segments from reduced oscillation frequency. Thus, consistent earlier computational models, developmental increase frequency due increasing intensity input circuitry. Overall, our results show locusts hatch fully formed system structurally functionally matches adult, despite its small size lack prior experience stimuli.

Язык: Английский

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Stimulus duration encoding occurs early in the moth olfactory pathway

Communications Biology, Год журнала: 2024, Номер 7(1)

Опубликована: Окт. 3, 2024

Язык: Английский

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Smell and Aftersmell: Fast Calcium Imaging Dynamics of Honey Bee Olfactory Coding

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Ноя. 21, 2023

Abstract Odour processing exhibits multiple parallels between vertebrate and invertebrate olfactory systems. Insects, in particular, have emerged as relevant models for studies because of the tractability their circuits. Here, we used fast calcium imaging to track activity projection neurons honey bee antennal lobe (AL) during stimulation at high temporal resolution. We observed a heterogeneity response profiles an abundance inhibitory activities, resulting various latencies stimulus- specific post-odour neural signatures. Recorded signals were fed mushroom body (MB) model constructed implementing fundamental features connectivity neurons, Kenyon cells (KC), MB output (MBON). The accounts increase odorant discrimination compared AL reveals recruitment two distinct KC populations that represent odorants aftersmell separate but temporally coherent objects. Finally, showed learning- induced modulation KC-to-MBON synapses can explain both variations associative learning scores across different conditioning protocols bees bees’ latency. Thus, it provides simple explanation how time contingency stimulus reward be encoded without need tracking. This study broadens our understanding coding bees. It demonstrates based on rules with real physiological data aspects odour learning.

Язык: Английский

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Stimulus duration encoding occurs early in the moth olfactory pathway

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2022, Номер unknown

Опубликована: Июль 22, 2022

Pheromones convey rich ethological information and guide insects’ search behavior. Insects navigating in turbulent environments are tasked with the challenge of coding temporal structure an odor plume, obliging recognition onset offset whiffs odor. The mechanisms that shape remain elusive. We designed a device to deliver sharp pheromone pulses simultaneously measured response dynamics from pheromone-tuned olfactory receptor neurons (ORNs) male moths Drosophila . show concentration-invariant stimulus duration encoding is implemented moth ORNs by spike frequency adaptation at two time scales. A linear-nonlinear model fully captures underlying neural computations offers insight into their biophysical mechanisms. use cis -vaccenyl acetate (cVA) only for very short distance communication not faced need encode statistics cVA plume. Their cVA-sensitive indeed unable odor-off events. Expression receptors indicates stimulus-offset independent. In ORNs, breaks down ( < 200 ms) whiffs. This physiological constraint matches behavioral latency switching upwind surge crosswind cast flight upon losing contact pheromone.

Язык: Английский

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