bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Сен. 5, 2024
Most of our understanding the fundamental processes mutation and recombination stems from a handful disparate model organisms pedigree studies mammals, with little known about other vertebrates. To gain broader comparative perspective, we focused on zebra finch (
Язык: Английский
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown
Опубликована: Янв. 27, 2023
Abstract The rates of appearance new mutations play a central role in evolution. However, mutational processes natural environments and their relationship with growth are largely unknown, particular tropical ecosystems high biodiversity. Here, we examined the somatic mutation landscapes two trees, Shorea laevis (slow-growing) S. leprosula (fast-growing), Borneo, Indonesia. Using newly-constructed genomes, identified greater number trees than temperate trees. In both species, observed linear increase physical distance between branches. found that rate accumulation per meter was 3.7-fold higher . This difference scaled slower compared to leprosula, resulting constant year species. We also neutral within an individual, but those transmitted next generation subject purifying selection. These findings suggest accumulate absolute time older have contribution towards generating genetic variation. Significance Statement significance our study lies discovery time-dependent long-lived independent rate. Through comparative analysis slow- fast-growing clock-like regardless rates. Although majority were restricted single branch, present multiple branches, likely during growth. Our make
Язык: Английский
Процитировано
6
Nucleic Acids Research, Год журнала: 2023, Номер 51(5), С. 2319 - 2332
Опубликована: Фев. 20, 2023
Abstract During mammalian spermatogenesis, the paternal genome is extensively remodelled via replacement of histones with protamines forming highly compact mature sperm nucleus. Compaction occurs in post-meiotic spermatids and accompanied by extensive double strand break (DSB) formation. We investigate epigenomic genomic context mouse spermatid DSBs, identifying primary sequence motifs, secondary DNA structures chromatin contexts associated this damage. Consistent previously published results we find DSBs positively short tandem repeats LINE elements. further show preferentially occur association (CA)n, (NA)n (RY)n repeats, predicted Z-DNA, are not G-quadruplexes, found regions low histone mark coverage engage remodelling/NHEJ factor BRD4. Locations incurring also distinct epigenetic profiles throughout later developmental stages: retaining sperm, susceptible to oxidative damage fragile two-cell like embryonic stem cell bound ZSCAN4 all co-localise each other. Our point a common ‘vulnerability code’ unifying several types occurring on during reproduction, potentially underpinned torsional changes remodelling.
Язык: Английский
Процитировано
5
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown
Опубликована: Апрель 14, 2023
Abstract Senescence, the deterioration of organismal function with advancing age, is a puzzling biological phenomenon. While actuarial senescence ( i.e. , age-dependent increases in mortality rates) well described across some taxa, reproductive age- dependent declines reproduction) less understood, especially males, mixed patterns reported studies. To examine evidence for male senescence, we investigated how age affects ejaculate traits non-human animals via meta-analysis yielding 1814 effect sizes from 379 We found no general pattern senescence. Instead, high heterogeneity reproduction changes animals. Some this (>10%) was associated factors. For example, there were taxonomical differences — sperm motility declined lab rodents and fish, whereas size improved bulls, insects. methodological factors also important explaining heterogeneity: studies sampling larger proportion species’ lifespan more likely to detect traits, emphasising need full life cycle species document Contrary predictions, reveal that sporadic. Our findings will help generate novel hypotheses identify effective approaches studying
Язык: Английский
Процитировано
5
Evolution, Год журнала: 2024, Номер 78(9), С. 1619 - 1632
Опубликована: Июнь 24, 2024
Abstract Advancing male age is often hypothesized to reduce both fertility and offspring quality due reproductive senescence. However, the effects of advancing on output are not always deleterious. For example, older fathers might buffer senescence by terminally investing in reproduction. Similarly, males that survive reproduce at an old carry alleles confer high viability (viability selection), which then inherited offspring, or have potential (selective disappearance). Differentiating these mechanisms requires integrated experimental study paternal survival performance, as well quality, currently lacking. Using a cross-sectional Drosophila melanogaster, we test conception (PAC) success, lifespans sons. We discover mating linked with decreased future survival, suggesting mating-induced mortality possibly being frail. find no evidence for terminal investment show does onset until their late-adult life. Additionally, father’s lifespan increases, his probability siring increases PAC treatments only. Lastly, sons born live longer than those younger selection. Collectively, our results suggest necessarily associated deleterious may even lead producing longer-lived offspring.
Язык: Английский
Процитировано
1
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Сен. 5, 2024
Most of our understanding the fundamental processes mutation and recombination stems from a handful disparate model organisms pedigree studies mammals, with little known about other vertebrates. To gain broader comparative perspective, we focused on zebra finch (
Язык: Английский
Процитировано
1