bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Апрель 1, 2024
Abstract
Background
Marine
organisms
with
sessile
adults
commonly
possess
motile
larval
stages
that
make
settlement
decisions
based
on
integrating
environmental
sensory
cues.
Phototaxis,
the
movement
toward
or
away
from
light,
is
a
common
behavioral
characteristic
of
aquatic
and
marine
metazoan
larvae,
algae,
protists,
fungi.
In
cnidarians,
genomic
investigations
planulae
larvae
have
been
conducted
in
anthozoans
(corals
sea
anemones)
scyphozoans
(true
jellyfish),
but
such
studies
are
presently
lacking
hydrozoans.
Here,
we
examined
genomics
phototaxis
hydrozoan
Hydractinia
symbiolongicarpus
.
Results
A
study
day
3
indicated
preferential
to
green
(523
nm)
blue
(470
wavelengths
not
red
(625
wavelengths.
developmental
transcriptome
where
planula
were
collected
four
time
points
for
RNA-seq
revealed
many
genes
critical
physiology
development
ciliary
photosensory
systems
dynamically
expressed
correspond
expression
phototactic
behavior.
Microscopical
using
immunohistochemistry
situ
hybridization
demonstrated
several
transcripts
predicted
function
photoreceptors,
including
cnidops
class
opsin,
CNG
ion
channel,
CRX-like
transcription
factor,
localize
ciliated
bipolar
neurons
aboral
neural
plexus,
which
associated
direction
site
settlement.
Conclusions
The
preference
displayed
by
consistent
shallow
sandy
habitats
they
experience
nature.
Our
add
further
evidence
similarities
between
cnidops-mediated
photoreceptors
hydrozoans
other
cnidarians
as
found
eyes
humans
bilaterians,
suggesting
aspects
their
shared
evolutionary
history.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(22), С. 12215 - 12215
Опубликована: Ноя. 14, 2024
The
retina
is
crucial
for
converting
light
into
neuronal
signals
visual
perception.
Understanding
the
retina’s
structure,
function,
and
development
essential
vision
research.
It
known
that
thyroid
hormone
(TH)
receptor
type
beta
2
(TRβ2)
a
key
element
in
regulation
of
cone
differentiation
retina,
but
other
elements
TH
signaling,
such
as
transporters
enzyme
deiodinases,
have
also
been
implicated
retinal
cell
survival.
In
present
study,
we
investigated
expression
profile
genes
involved
signaling
analyzed
impact
thyroidal
status
on
morphology,
opsin
expression,
death/proliferation
profile,
well
color
preference
behavior
during
early
zebrafish
larvae.
mRNA
analysis
dissected
whole
eyes
revealed
gradually
increase
eye
development,
with
dio3b
being
component
shows
most
dramatic
change.
Mutations
generated
by
CRISPR/CAS9
gene,
not
thrb
modifies
structure
retina.
Disruption
level
reduces
number
ganglion
layer,
increases
death,
preference,
emphasizing
critical
importance
precise
its
optimal
function.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Фев. 14, 2023
Abstract
Precise
wiring
within
sensory
systems
is
critical
for
the
accurate
transmission
of
information.
In
visual
system,
S-cone
photoreceptors
specialize
in
detecting
short-wavelength
light,
crucial
to
color
perception
and
environmental
cue
detection.
S-cones
form
specific
synapses
with
bipolar
cells
(SCBCs),
a
connection
that
remarkably
consistent
across
species.
Yet,
molecular
mechanisms
guiding
this
specificity
remain
unexplored.
To
address
this,
we
used
cone-dominant
ground
squirrel
deep-sequencing
cone
subtype
transcriptomes
identified
Nrxn3
as
an
essential
molecule
SCBC
synapse.
Using
transgenic
mouse
models,
further
examined
role
discovered
significant
reduction
connections
absence
.
This
finding
extends
known
functions
neurexins,
typically
associated
synapse
regulation,
by
highlighting
their
synaptic
first
time.
Moreover,
differentially
expressed
genes
here
pave
way
investigations
into
unique
subtypes.
