PyOKR: A Semi-Automated Method for Quantifying Optokinetic Reflex Tracking Ability DOI
James K. Kiraly, Scott C. Harris, Timour Al‐Khindi

и другие.

Journal of Visualized Experiments, Год журнала: 2024, Номер 206

Опубликована: Апрель 12, 2024

The study of behavioral responses to visual stimuli is a key component understanding system function. One notable response the optokinetic reflex (OKR), highly conserved innate behavior necessary for image stabilization on retina. OKR provides robust readout tracking ability and has been extensively studied understand circuitry function in animals from different genetic backgrounds. consists two phases: slow phase as eye follows stimulus edge plane compensatory fast saccade that resets position orbit. Previous methods gain quantification, although reliable, are labor intensive can be subjective or arbitrarily derived. To obtain more rapid reproducible quantification ability, we have developed novel semi-automated analysis program, PyOKR, allows two-dimensional motion any directional stimulus, addition being adaptable type video-oculography equipment. This method automated filtering, selection phases, modeling vertical horizontal vectors, movement gains relative speed, organization resultant data into usable spreadsheet statistical graphical comparisons. quantitative streamlined pipeline, readily accessible via PyPI import, direct measurement responses, thereby facilitating responses.

Язык: Английский

An ON-type direction-selective ganglion cell in primate retina DOI Creative Commons
Anna Y. Wang, Manoj Kulkarni,

Amanda J. McLaughlin

и другие.

Nature, Год журнала: 2023, Номер 623(7986), С. 381 - 386

Опубликована: Окт. 25, 2023

Abstract To maintain a stable and clear image of the world, our eyes reflexively follow direction in which visual scene is moving. Such gaze-stabilization mechanisms reduce blur as we move environment. In non-primate mammals, this behaviour initiated by retinal output neurons called ON-type direction-selective ganglion cells (ON-DSGCs), detect motion transmit signals to brainstem nuclei that drive compensatory eye movements 1 . However, ON-DSGCs have not yet been identified retina primates, raising possibility reflex mediated cortical areas. Here mined single-cell RNA transcriptomic data from primate identify candidate ON-DSGC. We then combined two-photon calcium imaging, molecular identification morphological analysis reveal population macaque retina. The morphology, signature GABA (γ-aminobutyric acid)-dependent underlie selectivity are highly conserved with those other mammals. further ON-DSGC human presence primates highlights need examine contribution subcortical normal aberrant gaze stabilization developing mature system.

Язык: Английский

Процитировано

20

Contrast Sensitivity of ON and OFF Human Retinal Pathways in Myopia DOI Creative Commons

Sabina Poudel,

Jianzhong Jin,

Hamed Rahimi-Nasrabadi

и другие.

Journal of Neuroscience, Год журнала: 2023, Номер 44(3), С. e1487232023 - e1487232023

Опубликована: Ноя. 28, 2023

The human visual cortex processes light and dark stimuli with ON OFF pathways that are differently modulated by luminance contrast. We have previously demonstrated cortical higher contrast sensitivity than the difference increases range (defined as maximum minus minimum in scene). Here, we demonstrate these ON–OFF differences already present retina retinal responses measured electroretinography more affected reductions electroencephalography. Moreover, show pathway become pronounced myopia, a disorder elongates eye blurs vision at far distance. find that, axial length across subjects, less responsive, slower response latency, sensitive, effective driving pupil constriction. Based on results, conclude myopia is associated deficit function decreases ability of to process low regulate illuminance bright environments.

Язык: Английский

Процитировано

18

Transformation of Motion Pattern Selectivity from Retina to Superior Colliculus DOI

Victor J. DePiero,

Zixuan Deng, Chen Chen

и другие.

Journal of Neuroscience, Год журнала: 2024, Номер 44(20), С. e1704232024 - e1704232024

Опубликована: Апрель 3, 2024

The superior colliculus (SC) is a prominent and conserved visual center in all vertebrates. In mice, the most superficial lamina of SC enriched with neurons that are selective for moving direction stimuli. Here, we study how these respond to complex motion patterns known as plaids, using two-photon calcium imaging awake male female mice. plaid pattern consists two superimposed sinusoidal gratings different directions, giving an apparent lies between directions component gratings. Most mouse robustly plaids show high selectivity but not its components. Pattern seen both excitatory inhibitory especially prevalent response large cross angles However, retinal inputs ambiguous their versus motion. Modeling suggests can arise from nonlinear transformation converging inputs. contrast, prevalence primary cortex (V1). These results demonstrate interesting difference V1 processing reveal important site encoding

Язык: Английский

Процитировано

4

Asymmetries in the Architecture of ON and OFF Arbors in ON–OFF Direction‐Selective Ganglion Cells DOI
Shaji Philip, Narendra Pratap Singh, Savin Viswanathan

и другие.

The Journal of Comparative Neurology, Год журнала: 2025, Номер 533(1)

Опубликована: Янв. 1, 2025

ABSTRACT Direction selectivity is a fundamental feature in the visual system. In retina, direction independently computed by ON and OFF circuits. However, advantages of extracting directional information from these two independent circuits are unclear. To gain insights, we examined ON–OFF direction‐selective ganglion cells (DSGCs), which recombine signals both Specifically, investigated dendritic architecture neurons with premise that asymmetries will provide insights into function. Scrutinizing dendrites dye‐filled DSGCs reveals arbors substantially denser. The increase density can be primarily attributed to higher branching seen arbors. Further, analysis previously published serial block‐face electron microscopy dataset revealed denser packed more bipolar synapses per unit length. These suggest DSGC preferentially magnifies synaptic drive pathway, potentially allowing it encode distinct pathway.

Язык: Английский

Процитировано

0

Distinct Developmental Programs Displayed by the Xenopus Tadpole Accessory Optic System and Retinotectal Projection DOI
Uwemedimo G. Udoh, Kaiyuan Zheng,

John R. Bruno

и другие.

Developmental Neurobiology, Год журнала: 2025, Номер 85(3)

Опубликована: Май 8, 2025

ABSTRACT The retinotectal projection, the direct synapse between retinal ganglion cells (RGCs) of eye and tectal neurons optic tectum, is a major component amphibian visual system. A model circuit formation, this projection has been studied in detail. There are, however, other retinorecipient targets that also comprise system such as pretectum ventral midbrain tegmentum. Understanding how these components form function will lead to more comprehensive understanding system, whole, assembles functions. Toward aim, here we describe functional development Xenopus tadpole accessory (AOS), synaptic connection RGC axons basal nucleus AOS highly conserved across vertebrates. It functions sensory side optokinetic optomotor reflexes, compensatory body movements, respectively, stabilize scene organism moves through it. Using an isolated brain preparation whole‐cell electrophysiological approaches, compared projection. We found two retinofugal projections display distinct developmental programs, which appear mirror their different Retinotectal synapses moved dynamic phase previously described NMDA receptor‐dependent refinement, process known sharpen retinotopic map thereby acuity. In contrast, appeared stable activity independent development, indicative hardwired circuit, built support reflexive behaviors.

Язык: Английский

Процитировано

0

Asymmetric retinal direction tuning predicts optokinetic eye movements across stimulus conditions DOI Creative Commons
Scott C. Harris, Felice A. Dunn

eLife, Год журнала: 2023, Номер 12

Опубликована: Март 17, 2023

Across species, the optokinetic reflex (OKR) stabilizes vision during self-motion. OKR occurs when ON direction-selective retinal ganglion cells (oDSGCs) detect slow, global image motion on retina. How oDSGC activity is integrated centrally to generate behavior remains unknown. Here, we discover mechanisms that contribute encoding in vertically tuned oDSGCs and leverage these findings empirically define signal transformation between output vertical behavior. We demonstrate contrast-sensitive asymmetric for types prefer opposite directions. These phenomena arise from interplay spike threshold nonlinearities differences synaptic input weights, including shifts balance of excitation inhibition. In behaving mice, neurophysiological observations, along with a central subtraction outputs, accurately predict trajectories across stimulus conditions. Thus, tuning competing sensory channels can critically shape

Язык: Английский

Процитировано

9

Endoplasmic reticulum stress-related deficits in calcium clearance promote neuronal dysfunction that is prevented by SERCA2 gene augmentation DOI Creative Commons
Yukihiro Shiga,

Aline Giselle Rangel Olguin,

Sana El Hajji

и другие.

Cell Reports Medicine, Год журнала: 2024, Номер unknown, С. 101839 - 101839

Опубликована: Ноя. 1, 2024

Язык: Английский

Процитировано

2

Tau P301S Transgenic Mice Develop Gait and Eye Movement Impairments That Mimic Progressive Supranuclear Palsy DOI Creative Commons
Rose B. Creed, Scott C. Harris, Sadhana Sridhar

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 23, 2024

Abstract Progressive supranuclear palsy (PSP) is a neurodegenerative disorder with an estimated prevalence of 5-7 people in 100,000. Clinically characterized by impairments gait, balance, and eye movements, as well aggregated Tau pathology, PSP leads to death approximately 5-8 years. No disease-modifying treatments are currently available. The contribution pathology the symptoms patients poorly understood, part due lack rodent model that recapitulates characteristic aspects PSP. Here, we assessed hTau.P301S mouse for key clinical features PSP, finding progressive balance gait coordination. Additionally, found fast vertical one most distinctive Across animals, motor control regions correlated deficits. These findings highlight utility hP301S modeling

Язык: Английский

Процитировано

1

PyOKR: A Semi-Automated Method for Quantifying Optokinetic Reflex Tracking Ability DOI
James K. Kiraly, Scott C. Harris, Timour Al‐Khindi

и другие.

Journal of Visualized Experiments, Год журнала: 2024, Номер 206

Опубликована: Апрель 12, 2024

The study of behavioral responses to visual stimuli is a key component understanding system function. One notable response the optokinetic reflex (OKR), highly conserved innate behavior necessary for image stabilization on retina. OKR provides robust readout tracking ability and has been extensively studied understand circuitry function in animals from different genetic backgrounds. consists two phases: slow phase as eye follows stimulus edge plane compensatory fast saccade that resets position orbit. Previous methods gain quantification, although reliable, are labor intensive can be subjective or arbitrarily derived. To obtain more rapid reproducible quantification ability, we have developed novel semi-automated analysis program, PyOKR, allows two-dimensional motion any directional stimulus, addition being adaptable type video-oculography equipment. This method automated filtering, selection phases, modeling vertical horizontal vectors, movement gains relative speed, organization resultant data into usable spreadsheet statistical graphical comparisons. quantitative streamlined pipeline, readily accessible via PyPI import, direct measurement responses, thereby facilitating responses.

Язык: Английский

Процитировано

0