Bone marrow adipogenic lineage precursors are the major regulator of bone resorption in adult mice DOI Creative Commons
Ling Qin, Jiawei Lu, Qi He

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Авг. 28, 2024

Abstract Bone resorption by osteoclasts is a critical step in bone remodeling, process important for maintaining homeostasis and repairing injured bone. We previously identified marrow mesenchymal subpopulation, adipogenic lineage precursors (MALPs), showed that its production of RANKL stimulates young mice using Adipoq-Cre. To exclude developmental defects to investigate the role MALPs-derived adult bone, we generated inducible reporter (Adipoq-CreER Tomato) deficient RANKLflox/flox, iCKO). Single cell-RNA sequencing data analysis, tracing, situ hybridization revealed Adipoq+ cells contain not only MALPs but also late progenitors capable osteogenic differentiation. However, RANKLmRNA was detected MALPs, cells. deficiency induced at 3 months age rapidly increased trabecular mass long bones as well vertebrae within 1 month due diminished had no effect on cortical Ovariectomy (OVX) loss both sites. depletion either before OVX or 6 weeks post protected restored mass. Furthermore, healing after drill-hole injury delayed iCKO mice. Together, our findings demonstrate play dominant controlling from essential turnover homeostasis, postmenopausal loss, repair.

Язык: Английский

Unlocking the Therapeutic Potential of Adipose-Derived Stem Cell Secretome in Oral and Maxillofacial Medicine: A Composition-Based Perspective DOI Creative Commons
Chiara Giannasi, Francesca Cadelano, Elena Della Morte

и другие.

Biology, Год журнала: 2024, Номер 13(12), С. 1016 - 1016

Опубликована: Дек. 5, 2024

The adipose-derived stem cell (ADSC) secretome is widely studied for its immunomodulatory and regenerative properties, yet potential in maxillofacial medicine remains largely underexplored. This review takes a composition-driven approach, beginning with list of chemokines, cytokines, receptors, inflammatory growth factors quantified the ADSC to infer applications this medical field. First, literature confirmed presence 107 bioactive ADSCs or other types mesenchymal cells. was then analyzed using Search Tool Retrieval Interacting Genes/Proteins (STRING) software, revealing 844 enriched biological processes. From these, key processes were categorized into three major clinical application areas: immunoregulation (73 factors), bone regeneration (13 wound healing soft tissue (27 several relevant more than one area. most molecules discussed context existing explore their therapeutic based on available evidence. Among TGFB1, IL10, CSF2 have been shown modulate immune responses, while OPG, IL6, HGF, TIMP1 contribute repair. Although holds great promise oral medicine, further research needed optimize validate efficacy.

Язык: Английский

Процитировано

1
Damage of the Bone Marrow Stromal Precursors in Patients with Acute Leukemia at the Onset of the Disease and During Treatment DOI Open Access
A. V. Sadovskaya, Н. А. Петинати, Irina N. Shipounova

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(24), С. 13285 - 13285

Опубликована: Дек. 11, 2024

In patients with acute leukemia (AL), malignant cells and therapy modify the properties of multipotent mesenchymal stromal (MSCs) their descendants, reducing ability to maintain normal hematopoiesis. The aim this work was elucidate alterations in MSCs at onset after AL. study included obtained from bone marrow 78 AL (42 AML 36 ALL) healthy donors. MSC growth characteristics, gene expression pattern, proteome secretome were studied using appropriate methods. concentration marrow, proliferative potential, several genes, proteomes secretomes altered AL-MSCs. Stromal progenitors had been affected differently ALL patients. remission, functions remain impaired despite absence tumor maintenance benign hematopoietic cells. causes crucial and, a large extent, irreversible changes MSCs.

Язык: Английский

Процитировано

1
Skeletal adaptation to mechanical cues during homeostasis and repair: the niche, cells, and molecular signaling DOI Creative Commons
Pablo J. Atria, Alesha B. Castillo

Frontiers in Physiology, Год журнала: 2023, Номер 14

Опубликована: Окт. 17, 2023

Bones constantly change and adapt to physical stress throughout a person’s life. Mechanical signals are important regulators of bone remodeling repair by activating skeletal stem progenitor cells (SSPCs) proliferate differentiate into bone-forming osteoblasts using molecular signaling mechanisms not yet fully understood. SSPCs reside in dynamic specialized microenvironment called the niche , where external integrate influence cell maintenance, behavior fate determination. The nature bone, including its cellular extracellular makeup regulatory signals, is completely which niche, with all components complexity, modulated mechanical during homeostasis virtually unknown. This review summarizes current view involved adaptation repair, an emphasis on identifying novel targets for prevention treatment age-related loss hard-to-heal fractures.

Язык: Английский

Процитировано

1
Adiponectin expressing skeletal stem/progenitor cells in the bone and bone marrow homeostasis DOI Creative Commons
Biagio Palmisano, Alessandro Corsi, Mara Riminucci

и другие.

Current Opinion in Endocrine and Metabolic Research, Год журнала: 2024, Номер 35, С. 100524 - 100524

Опубликована: Апрель 23, 2024

The bone marrow stroma supports hematopoiesis while replenishing osteoblasts and adipocytes. These functions rely on different stromal cell populations identified by lineage tracing, phenotypic markers single-cell transcriptomic analysis. A subset expressing Adiponectin (Adipoq+) has been reported studies in mice. However, there is a general agreement the kinetics of Adipoq+ cells during mouse growth, their ability to generate adipocytes support vasculature, contrasting results have osteogenic activity. In this work, we review available data cells, with special focus experimental evidence demonstrating osteoprogenitor function potential reasons for divergence observed among studies. Furthermore, discuss overlap other context widely recognized adipogenic bias that characterizes many stem populations.

Язык: Английский

Процитировано

0
Bone marrow adipogenic lineage precursors are the major regulator of bone resorption in adult mice DOI Creative Commons
Ling Qin, Jiawei Lu, Qi He

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Авг. 28, 2024

Abstract Bone resorption by osteoclasts is a critical step in bone remodeling, process important for maintaining homeostasis and repairing injured bone. We previously identified marrow mesenchymal subpopulation, adipogenic lineage precursors (MALPs), showed that its production of RANKL stimulates young mice using Adipoq-Cre. To exclude developmental defects to investigate the role MALPs-derived adult bone, we generated inducible reporter (Adipoq-CreER Tomato) deficient RANKLflox/flox, iCKO). Single cell-RNA sequencing data analysis, tracing, situ hybridization revealed Adipoq+ cells contain not only MALPs but also late progenitors capable osteogenic differentiation. However, RANKLmRNA was detected MALPs, cells. deficiency induced at 3 months age rapidly increased trabecular mass long bones as well vertebrae within 1 month due diminished had no effect on cortical Ovariectomy (OVX) loss both sites. depletion either before OVX or 6 weeks post protected restored mass. Furthermore, healing after drill-hole injury delayed iCKO mice. Together, our findings demonstrate play dominant controlling from essential turnover homeostasis, postmenopausal loss, repair.

Язык: Английский

Процитировано

0