Neural-circuit basis of song preference learning in fruit flies DOI Creative Commons

Keisuke Imoto,

Yuki Ishikawa, Yoshinori Aso

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Окт. 26, 2023

Abstract As observed in human language learning and song birds, the fruit fly Drosophila melanogaster changes its’ auditory behaviors according to prior sound experiences. Female flies that have heard male courtship songs of same species are less responsive different species. This phenomenon, known as preference flies, requires GABAergic input pC1 neurons central brain, with these playing a key role mating behavior by integrating multimodal sensory internal information. The neural circuit basis this input, however, has not yet been identified. Here, we find pCd-2 neurons, totaling four cells per hemibrain expressing sex-determination gene doublesex , provide for learning. First, RNAi-mediated knockdown GABA production abolished Second, directly, many cases mutually, connect suggesting existence reciprocal circuits between neurons. Finally, dopaminergic inputs necessary Together, study suggests serve state-integrated hub, allowing flexible control over female copulation. Consequently, provides model underlies experience-dependent plasticity. Significance To suitable mate, an organism must adapt its based on past In case assessments signals, which contain information about status sender, experience dependent. show brain modulate female’s response depending her previous These exhibit feedback lateral inhibition motifs, regulated inputs. While effects experiences responsiveness extensively studied other species, our research advances use dissecting circuitry underlying plasticity at single-cell resolution.

Язык: Английский

A searchable image resource of Drosophila GAL4 driver expression patterns with single neuron resolution DOI Creative Commons
Geoffrey W. Meissner, Aljoscha Nern, Zachary Dorman

и другие.

eLife, Год журнала: 2023, Номер 12

Опубликована: Фев. 23, 2023

Precise, repeatable genetic access to specific neurons via GAL4/UAS and related methods is a key advantage of

Язык: Английский

Процитировано

85
Neural circuit mechanisms underlying context-specific halting in Drosophila DOI Creative Commons

Neha Sapkal,

Nino Mancini,

Divya Sthanu Kumar

и другие.

Nature, Год журнала: 2024, Номер 634(8032), С. 191 - 200

Опубликована: Окт. 2, 2024

Язык: Английский

Процитировано

16
Hunger- and thirst-sensing neurons modulate a neuroendocrine network to coordinate sugar and water ingestion DOI Creative Commons

Amanda J González Segarra,

Gina Pontes, Nicholas Jourjine

и другие.

eLife, Год журнала: 2023, Номер 12

Опубликована: Июнь 15, 2023

Consumption of food and water is tightly regulated by the nervous system to maintain internal nutrient homeostasis. Although generally considered independently, interactions between hunger thirst drives are important coordinate competing needs. In Drosophila , four neurons called interoceptive subesophageal zone (ISNs) respond intrinsic signals oppositely regulate sucrose ingestion. Here, we investigate neural circuit downstream ISNs examine how ingestion based on Utilizing recently available fly brain connectome, find that synapse with a novel cell-type bilateral T-shaped neuron (BiT) projects neuroendocrine centers. vivo manipulations revealed BiT regulates sugar Neuroendocrine cells include several peptide-releasing peptide-sensing neurons, including insulin producing (IPCs), crustacean cardioactive peptide (CCAP) CCHamide-2 receptor isoform RA (CCHa2R-RA) neurons. These contribute differentially water, IPCs CCAP regulating ingestion, CCHa2R-RA modulating only Thus, decision consume or occurs via regulation broad peptidergic network integrates nutritional state generate nutrient-specific

Язык: Английский

Процитировано

12
Connectomic analysis of taste circuits in Drosophila DOI Creative Commons

Sydney R. Walker,

Marco Peña-Garcia,

Anita V. Devineni

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Фев. 12, 2025

Abstract Our sense of taste is critical for regulating food consumption. The fruit fly Drosophila represents a highly tractable model to investigate mechanisms processing, but circuits beyond sensory neurons are largely unidentified. Here, we use whole-brain connectome the organization circuits. We trace pathways from four populations that detect different modalities and project subesophageal zone (SEZ), primary region brain. find second-order primarily located within SEZ segregated by modality, whereas third-order have more projections outside overlap between modalities. Taste out innervate regions implicated in feeding, olfactory learning. analyze interconnections pathways, characterize modality-dependent differences neuron properties, identify other types inputs onto computational simulations relate neuronal connectivity predicted activity. These studies provide insight into architecture

Язык: Английский

Процитировано

0
Neural circuit mechanisms underlying context-specific halting inDrosophila DOI Creative Commons

Neha Sapkal,

Nino Mancini,

Divya Sthanu Kumar

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Сен. 27, 2023

Abstract Walking is a complex motor program involving coordinated and distributed activity across the brain spinal cord. Halting appropriately at correct time critical but often overlooked component of walking control. While recent studies have delineated specific genetically defined neuronal populations in mouse brainstem that drive different types halting 1–3 , underlying neural circuit mechanisms responsible for overruling competing walking-state to generate context-appropriate halting, remain unclear. Here, we elucidate two fundamental by which Drosophila implement halting. The first mechanism (“walk-OFF” mechanism) relies on GABAergic neurons inhibit descending commands brain, while second (“brake” excitatory cholinergic nerve-cord lead an active arrest stepping movements. Using connectome-informed models 4–6 functional studies, show deploy “walk-OFF” distinct walking-promotion neurons, leading differential forward-walking or steering. “brake” other hand, override all simultaneously inhibiting promoting pathways increasing resistance leg-joints leg movements stance phase walking. We characterized ethologically relevant behavioral contexts were used animal mutually exclusive manner: pathway was engaged during feeding, grooming. Furthermore, this knowledge targets allowed us use connectomics predict novel could be contexts.

Язык: Английский

Процитировано

6
Two neuropeptides that promote blood-feeding inAnopheles stephensimosquitoes DOI Creative Commons
Prashali Bansal,

Roshni Pillai,

DB Pooja

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 15, 2024

Animals routinely need to make decisions about what eat and when. These are influenced not only by the availability quality of food but also internal state animal, which needs compute give weights these different variables before making a choice. Feeding preferences female mosquitoes exemplify this behavioural plasticity. Both male usually feed on carbohydrate-rich sources nectar or sap, feeds blood, is essential for egg development. This blood-appetite modulated across female’s reproductive cycle, yet little known factors that bring it about. We show Anopheles stephensi females, major vector urban Malaria Indian sub-continent West-Africa, suppress blood-feeding between blood-meal until oviposition. Transcriptomics blood-deprived sated An. mid-brains allowed us shortlist several candidate genes might promote behaviour. Through dsRNA-mediated knockdown nine them, we identified two neuropeptides – short Neuropeptide F (sNPF) RYamide blood-feeding. find neuromodulators expressed in non-overlapping subsets neurons brain suggesting behaviour likely multiple mechanisms acting neurons.

Язык: Английский

Процитировано

1
Neural-circuit basis of song preference learning in fruit flies DOI Creative Commons

Keisuke Imoto,

Yuki Ishikawa, Yoshinori Aso

и другие.

iScience, Год журнала: 2024, Номер 27(7), С. 110266 - 110266

Опубликована: Июнь 13, 2024

As observed in human language learning and song birds, the fruit fly

Язык: Английский

Процитировано

1
An integrative sensor of body states: how the mushroom body modulates behavior depending on physiological context DOI Open Access
Raquel Suárez-Grimalt, Ilona C Grunwald Kadow, Lisa Scheunemann

и другие.

Learning & Memory, Год журнала: 2024, Номер 31(5), С. a053918 - a053918

Опубликована: Май 1, 2024

The brain constantly compares past and present experiences to predict the future, thereby enabling instantaneous future behavioral adjustments. Integration of external information with animal's current internal needs state represents a key challenge nervous system. Recent advancements in dissecting function Drosophila mushroom body (MB) at single-cell level have uncovered its three-layered logic parallel systems conveying positive negative values during associative learning. This review explores lesser-known role MB detecting integrating states such as hunger, thirst, sleep, ultimately modulating motivation sensory-driven decisions based on physiological fly. State-dependent signals predominantly affect activity modulatory input neurons (dopaminergic, serotoninergic, octopaminergic), but also induce plastic changes directly intrinsic output neurons. Thus, emerges tightly regulated relay station insect brain, orchestrating neuroadaptations due leading short- long-lasting behavior. While these adaptations are crucial ensure fitness survival, recent findings underscore how circuit motifs may reflect fundamental design principles that contribute maladaptive behaviors addiction or depression-like symptoms.

Язык: Английский

Процитировано

1
Connectomic analysis of taste circuits inDrosophila DOI Creative Commons

Sydney R. Walker,

Marco Peña-Garcia,

Anita V. Devineni

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 15, 2024

Our sense of taste is critical for regulating food consumption. The fruit fly

Язык: Английский

Процитировано

1
Two Neuropeptides that Promote Blood-Feeding in Anopheles Stephensi Mosquitoes DOI

Prashali Bansal,

Roshni Pillai,

Pooja DB

и другие.

Опубликована: Янв. 1, 2024

Animals routinely need to make decisions about what eat and when. These are influenced not only by the availability quality of food but also internal state animal, which needs compute give weights these different variables before making a choice. Feeding preferences female mosquitoes exemplify this behavioural plasticity. Both male usually feed on carbohydrate-rich sources nectar or sap, feeds blood, is essential for egg development. This blood-appetite modulated across female's reproductive cycle, yet little known factors that bring it about.We show Anopheles stephensi females, major vector urban Malaria Indian sub-continent West-Africa, suppress blood-feeding between blood-meal until oviposition. Transcriptomics blood-deprived sated An. midbrains allowed us shortlist several candidate genes might promote bloodfeeding behaviour. Through dsRNA-mediated knockdown nine them, we identified two neuropeptides – short Neuropeptide F (sNPF) RYamide - blood-feeding. We find neuromodulators expressed in nonoverlapping subsets neurons brain suggesting behaviour likely multiple mechanisms acting neurons.

Язык: Английский

Процитировано

0