The
hypothalamus
regulates
fundamental
metabolic
processes
by
controlling
functions
as
varied
food
intake,
body
temperature,
and
hormone
release.
As
the
of
are
controlled
specific
subsets
neuronal
populations,
ability
to
isolate
them
provides
a
major
tool
for
studying
mechanisms.
In
this
regard,
complexity
poses
exceptional
challenges.
For
these
reasons,
new
techniques,
such
Magnetic-Activated
Cell
Sorting
(MACS),
have
been
explored.
This
paper
describes
application
magnetic-activated
cell
sorting
(MACS)
using
microbead
technology
targeted
population
from
prenatal
mice
brains.
technique
is
simple
guarantees
highly
pure
viable
primary
hypothalamic
neuron
culture
with
high
reproducibility.
gently
dissociated,
neurons
selectively
isolated
separated
glial
cells,
finally,
antibody
surface
marker,
interest
selected.
Once
isolated,
can
be
used
investigate
their
morphological,
electrical,
endocrine
characteristics
responses
in
normal
or
pathological
conditions.
Furthermore,
given
variegated
roles
regulating
feeding,
metabolism,
stress,
sleep,
motivation,
closer
look
at
region-specific
may
provide
insight
into
tasks
complex
environment.
Communications Biology,
Год журнала:
2024,
Номер
7(1)
Опубликована: Апрель 13, 2024
Abstract
Visceral
hypersensitivity,
a
common
clinical
manifestation
of
irritable
bowel
syndrome,
may
contribute
to
the
development
chronic
visceral
pain,
which
is
major
challenge
for
both
patients
and
health
providers.
Neural
circuits
in
brain
encode,
store,
transfer
pain
information
across
regions.
In
this
review,
we
focus
on
anterior
cingulate
cortex
paraventricular
nucleus
hypothalamus
highlight
progress
identifying
neural
involved
pain.
We
also
discuss
several
circuit
mechanisms
emphasize
importance
cross-species,
multiangle
approaches
identification
specific
neurons
determining
that
control
Frontiers in Behavioral Neuroscience,
Год журнала:
2025,
Номер
19
Опубликована: Фев. 24, 2025
Bioinspired
robots
are
machines
which
reproduce
structural
or
functional
features
of
a
living
organism.
In
particular,
the
bioinspired
animals
can
be
more
specifically
defined
as
zooinspired
robots.
Currently,
applications
animal
various
and
range
across
different
fields,
such
as,
for
instance,
nature
conservation,
search
rescue
humans
after
natural
man-made
disasters,
exploration
extraterrestrial
environments
robotic
pets
elderly
people
under
care
dementia.
Several
species
have
been
imitated
up
to
now,
from
lizards
butterflies,
fish
dogs.
Animal
used
investigate
social
behavior
an
through
animal-robot
interactions
called
ethorobots.
Intriguingly,
ethorobots
able
in
laboratory
behaviors
that
generally
produced
spontaneously
difficult
impossible
evoke
modulate
captive
animals,
makes
these
particularly
useful
tools
experimental
ethology
ethological
neuroscience.
Rodents,
primarily
mice
rats,
most
common
model
biomedical
research.
Coherently
with
importance
scientific
research,
rats
attracting
increasing
efforts
robotics
over
course
past
five
decades.
The
technological
advancement
will
make
their
employment
research
increasingly
useful.
However,
clear
should
identified
order
challenge
engineers
design
serve
scopes.
present
work,
we
describe
possible
practical
mouse
behavioral
testing
six
domains,
namely
courtship,
parental
care,
antipredatory
behavior,
helping
predation
territory
defense-related
aggression.
outline
how
could
employed
interact
series
specific
tests
behavior.
Finally,
conclusion
consider
ethical
epistemological
advantages
use
Indeed,
benefit
on
both
terms
optimized
welfare
tested
subjects
extended
opportunities
designing
due
unprecedented
control
independent
variables.
Journal of Visualized Experiments,
Год журнала:
2023,
Номер
198
Опубликована: Авг. 4, 2023
The
hypothalamus
regulates
fundamental
metabolic
processes
by
controlling
functions
as
varied
food
intake,
body
temperature,
and
hormone
release.
As
the
of
are
controlled
specific
subsets
neuronal
populations,
ability
to
isolate
them
provides
a
major
tool
for
studying
mechanisms.
In
this
regard,
complexity
poses
exceptional
challenges.
For
these
reasons,
new
techniques,
such
Magnetic-Activated
Cell
Sorting
(MACS),
have
been
explored.
This
paper
describes
application
magnetic-activated
cell
sorting
(MACS)
using
microbead
technology
targeted
population
from
prenatal
mice
brains.
technique
is
simple
guarantees
highly
pure
viable
primary
hypothalamic
neuron
culture
with
high
reproducibility.
gently
dissociated,
neurons
selectively
isolated
separated
glial
cells,
finally,
antibody
surface
marker,
interest
selected.
Once
isolated,
can
be
used
investigate
their
morphological,
electrical,
endocrine
characteristics
responses
in
normal
or
pathological
conditions.
Furthermore,
given
variegated
roles
regulating
feeding,
metabolism,
stress,
sleep,
motivation,
closer
look
at
region-specific
may
provide
insight
into
tasks
complex
environment.
