Nitric oxide modulates contrast suppression in a subset of mouse retinal ganglion cells DOI Creative Commons
Dominic Gonschorek, Matías A. Goldin, Jonathan Oesterle

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Дек. 23, 2023

Neuromodulators have major influences on the regulation of neural circuit activity across nervous system. Nitric oxide (NO) has been shown to be a prominent neuromodulator in many circuits and extensively studied retina. Here, it associated with light adaptation, gain control, gap junctional coupling, but its effect retinal output, specifically different types ganglion cells (RGCs), is still poorly understood. In this study, we used two-photon Ca 2+ imaging multi-electrode array (MEA) recordings measure light-evoked RGCs cell layer ex vivo mouse This approach allowed us investigate neuromodulatory effects NO type-level. Our findings reveal that selectively modulates suppression temporal responses distinct subset contrast-suppressed RGC types, increasing their without altering spatial properties receptive fields. Given under photopic conditions, release triggered by quick changes levels, propose these signal fast contrast higher visual regions. Remarkably, found about one-third recorded using imaging, exhibited consistent, type-specific adaptational response throughout an experiment, independent NO. By employing sequential-recording paradigm, could disentangle those additional from drug-induced modulations. Taken together, our research highlights selective emphasizes need considering non-pharmacological changes, like such study designs.

Язык: Английский

A chromatic feature detector in the retina signals visual context changes DOI Creative Commons
Larissa Höfling, Klaudia P. Szatko, Christian Behrens

и другие.

eLife, Год журнала: 2024, Номер 13

Опубликована: Окт. 4, 2024

The retina transforms patterns of light into visual feature representations supporting behaviour. These are distributed across various types retinal ganglion cells (RGCs), whose spatial and temporal tuning properties have been studied extensively in many model organisms, including the mouse. However, it has difficult to link potentially nonlinear transformations natural inputs specific ethological purposes. Here, we discover a selectivity chromatic contrast an RGC type that allows detection changes context. We trained convolutional neural network (CNN) on large-scale functional recordings responses mouse movies, then used this search silico for stimuli maximally excite distinct RGCs. This procedure predicted centre colour opponency transient suppressed-by-contrast (tSbC) RGCs, cell function is being debated. confirmed experimentally these indeed responded very selectively Green-OFF, UV-ON contrasts. was characteristic transitions from ground sky scene, as might be elicited by head or eye movements horizon. Because tSbC performed best among all at reliably detecting transitions, suggest role providing contextual information (i.e. ground) necessary selection appropriate behavioural other stimuli, such looming objects. Our work showcases how combination experiments with computational modelling discovering novel stimulus identifying their potential relevance.

Язык: Английский

Процитировано

9
Towards a Foundation Model of the Mouse Visual Cortex DOI Creative Commons
Eric Wang, Paul G. Fahey, Zhuokun Ding

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Март 24, 2023

The complexity of neural circuits makes it challenging to decipher the brain’s algorithms intelligence. Recent break-throughs in deep learning have produced models that accurately simulate brain activity, enhancing our understanding computational objectives and coding. However, these struggle generalize beyond their training distribution, limiting utility. emergence foundation models, trained on vast datasets, has introduced a new AI paradigm with remarkable generalization capabilities. We collected large amounts activity from visual cortices multiple mice model predict neuronal responses arbitrary natural videos. This generalized minimal successfully predicted across various stimulus domains, such as coherent motion noise patterns. It could also be adapted tasks prediction, predicting anatomical cell types, dendritic features, connectivity within MICrONS functional connectomics dataset. Our work is crucial step toward building models. As neuroscience accumulates larger, multi-modal will uncover statistical regularities, enabling rapid adaptation accelerating research.

Язык: Английский

Процитировано

16
Nitric oxide modulates contrast suppression in a subset of mouse retinal ganglion cells DOI Creative Commons
Dominic Gonschorek, Matías A. Goldin, Jonathan Oesterle

и другие.

eLife, Год журнала: 2025, Номер 13

Опубликована: Янв. 9, 2025

Neuromodulators have major influences on the regulation of neural circuit activity across nervous system. Nitric oxide (NO) has been shown to be a prominent neuromodulator in many circuits and extensively studied retina. Here, it associated with light adaptation, gain control, gap junctional coupling, but its effect retinal output, specifically different types ganglion cells (RGCs), is still poorly understood. In this study, we used two-photon Ca 2+ imaging multi-electrode array (MEA) recordings measure light-evoked RGCs cell layer ex vivo mouse This approach allowed us investigate neuromodulatory effects NO type-level. Our findings reveal that selectively modulates suppression temporal responses distinct subset contrast-suppressed RGC types, increasing their without altering spatial properties receptive fields. Given under photopic conditions, release triggered by quick changes levels, propose these signal fast contrast higher visual regions. Remarkably, found about one-third recorded using imaging, exhibited consistent, type-specific adaptational response throughout an experiment, independent NO. By employing sequential-recording paradigm, could disentangle those additional from drug-induced modulations. Taken together, our research highlights selective emphasizes need considering non-pharmacological changes, like such study designs.

Язык: Английский

Процитировано

0
Mammalian Retinal Bipolar Cells: Morphological Identification and Systematic Classification in Rabbit Retina With a Comparative Perspective DOI
Edward V. Famiglietti

The Journal of Comparative Neurology, Год журнала: 2025, Номер 533(2)

Опубликована: Фев. 1, 2025

ABSTRACT Retinal bipolar cells (BCs) convey visual signals from photoreceptors to more than 50 types of rabbit retinal ganglion (Famiglietti, 2020). More 40 years ago, 10–11 were recognized in and cat retinas 1981). Twenty later, 10 identified mouse, rat, monkey, while recent molecular genetic studies indicate that there are 15 cell mouse retina (Shekhar et al., 2016). The present detailed study 800 11 Golgi‐impregnated indicates 14–16 cone one type rod retina. These have been carefully analyzed terms dendritic axonal morphology axon terminal stratification with respect fiducial starburst amacrine cells. In fortuitous proximity, several can be related by contacts suggestive synaptic connection. results compared other Homologies monkey considered functional terms.

Язык: Английский

Процитировано

0
Task-specific regional circuit adaptations in distinct mouse retinal ganglion cells DOI Creative Commons
Jonathan Oesterle,

Yanli Ran,

Paul Stahr

и другие.

Science Advances, Год журнала: 2025, Номер 11(17)

Опубликована: Апрель 23, 2025

In the mouse retina, sustained ON alpha (sONα) retinal ganglion cells (RGCs) have different dendritic and receptive field sizes along nasotemporal axis, with temporal sONα RGCs likely playing a role in visually guided hunting. Thus, we hypothesized that this cell type also exhibits regional adaptations signal processing these are advantageous for prey capture. Here, measured signals from individual at locations. We both postsynaptic Ca 2+ dendrites presynaptic glutamate bipolar (BCs). found exhibit, addition to sustained-ON only weak surrounds, strong surround suppression, which were not present nasal RGCs. This difference was inputs BCs. Last, using population models an encoder-decoder paradigm, showed might be beneficial detecting crickets hunting behavior.

Язык: Английский

Процитировано

0
Nitric oxide modulates contrast suppression in a subset of mouse retinal ganglion cells DOI Creative Commons
Dominic Gonschorek, Matías A. Goldin, Jonathan Oesterle

и другие.

eLife, Год журнала: 2024, Номер 13

Опубликована: Июнь 10, 2024

Neuromodulators have major influences on the regulation of neural circuit activity across nervous system. Nitric oxide (NO) has been shown to be a prominent neuromodulator in many circuits and extensively studied retina. Here, it associated with light adaptation, gain control, gap junctional coupling, but its effect retinal output, specifically different types ganglion cells (RGCs), is still poorly understood. In this study, we used two-photon Ca 2+ imaging multi-electrode array (MEA) recordings measure light-evoked RGCs cell layer ex vivo mouse This approach allowed us investigate neuromodulatory effects NO type-level. Our findings reveal that selectively modulates suppression temporal responses distinct subset contrast-suppressed RGC types, increasing their without altering spatial properties receptive fields. Given under photopic conditions, release triggered by quick changes levels, propose these signal fast contrast higher visual regions. Remarkably, found about one-third recorded using imaging, exhibited consistent, type-specific adaptational response throughout an experiment, independent NO. By employing sequential-recording paradigm, could disentangle those additional from drug-induced modulations. Taken together, our research highlights selective emphasizes need considering non-pharmacological changes, like such study designs.

Язык: Английский

Процитировано

1
Asymmetric distribution of color-opponent response types across mouse visual cortex supports superior color vision in the sky DOI Creative Commons
Katrin Franke, Chenchen Cai, Kayla Ponder

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Июнь 5, 2023

Color is an important visual feature that informs behavior, and the retinal basis for color vision has been studied across various vertebrate species. While many studies have investigated how information processed in brain areas of primate species, we limited understanding it organized beyond retina other including most dichromatic mammals. In this study, systematically characterized represented primary cortex (V1) mice. Using large-scale neuronal recordings a luminance noise stimulus, found more than third neurons mouse V1 are color-opponent their receptive field center, while surround predominantly captures contrast. Furthermore, color-opponency especially pronounced posterior encodes sky, matching statistics natural scenes experienced by unsupervised clustering, demonstrate asymmetry representations can be explained uneven distribution green-On/UV-Off response types upper field. Finally, simple model with scene-inspired parametric stimuli shows may enhance detection “predatory”-like dark UV-objects noisy daylight scenes. The results from study highlight relevance processing system contribute to our hierarchy

Язык: Английский

Процитировано

2
Nitric oxide modulates contrast suppression in a subset of mouse retinal ganglion cells DOI Open Access
Dominic Gonschorek, Matías A. Goldin, Jonathan Oesterle

и другие.

Опубликована: Дек. 13, 2024

Neuromodulators have major influences on the regulation of neural circuit activity across nervous system. Nitric oxide (NO) has been shown to be a prominent neuromodulator in many circuits and extensively studied retina. Here, it associated with light adaptation, gain control, gap junctional coupling, but its effect retinal output, specifically different types ganglion cells (RGCs), is still poorly understood. In this study, we used two-photon Ca 2+ imaging multi-electrode array (MEA) recordings measure light-evoked RGCs cell layer ex vivo mouse This approach allowed us investigate neuromodulatory effects NO type-level. Our findings reveal that selectively modulates suppression temporal responses distinct subset contrast-suppressed RGC types, increasing their without altering spatial properties receptive fields. Given under photopic conditions, release triggered by quick changes levels, propose these signal fast contrast higher visual regions. Remarkably, found about one-third recorded using imaging, exhibited consistent, type-specific adaptational response throughout an experiment, independent NO. By employing sequential-recording paradigm, could disentangle those additional from drug-induced modulations. Taken together, our research highlights selective emphasizes need considering non-pharmacological changes, like such study designs.

Язык: Английский

Процитировано

0
Nitric oxide modulates contrast suppression in a subset of mouse retinal ganglion cells DOI Creative Commons
Dominic Gonschorek, Matías A. Goldin, Jonathan Oesterle

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Дек. 23, 2023

Neuromodulators have major influences on the regulation of neural circuit activity across nervous system. Nitric oxide (NO) has been shown to be a prominent neuromodulator in many circuits and extensively studied retina. Here, it associated with light adaptation, gain control, gap junctional coupling, but its effect retinal output, specifically different types ganglion cells (RGCs), is still poorly understood. In this study, we used two-photon Ca 2+ imaging multi-electrode array (MEA) recordings measure light-evoked RGCs cell layer ex vivo mouse This approach allowed us investigate neuromodulatory effects NO type-level. Our findings reveal that selectively modulates suppression temporal responses distinct subset contrast-suppressed RGC types, increasing their without altering spatial properties receptive fields. Given under photopic conditions, release triggered by quick changes levels, propose these signal fast contrast higher visual regions. Remarkably, found about one-third recorded using imaging, exhibited consistent, type-specific adaptational response throughout an experiment, independent NO. By employing sequential-recording paradigm, could disentangle those additional from drug-induced modulations. Taken together, our research highlights selective emphasizes need considering non-pharmacological changes, like such study designs.

Язык: Английский

Процитировано

0