Quiescent horizontal basal stem cells act as a niche for olfactory neurogenesis in a mouse 3D organoid model DOI Creative Commons
Juliana Gutschow Gameiro, Constantin A. Hintschich,

Agnès Dekeyser

и другие.

Cell Reports Methods, Год журнала: 2025, Номер unknown, С. 101055 - 101055

Опубликована: Май 1, 2025

Язык: Английский

Simultaneous single-cell three-dimensional genome and gene expression profiling uncovers dynamic enhancer connectivity underlying olfactory receptor choice DOI Creative Commons
Honggui Wu, Jiankun Zhang, Fanchong Jian

и другие.

Nature Methods, Год журнала: 2024, Номер 21(6), С. 974 - 982

Опубликована: Апрель 15, 2024

Abstract The simultaneous measurement of three-dimensional (3D) genome structure and gene expression individual cells is critical for understanding a genome’s structure–function relationship, yet this challenging existing methods. Here we present ‘Linking mRNA to Chromatin Architecture (LiMCA)’, which jointly profiles the 3D transcriptome with exceptional sensitivity from low-input materials. Combining LiMCA our high-resolution scATAC-seq assay, METATAC, successfully characterized chromatin accessibility, as well paired structures information, developing olfactory sensory neurons. We expanded repertoire known receptor (OR) enhancers discovered unexpected rules their dynamics: OR genes are most accessible during early differentiation. Furthermore, revealed dynamic spatial relationship between ORs behind stepwise expression. These findings offer valuable insights into how connectivity dynamically orchestrate ‘one neuron–one receptor’ selection process.

Язык: Английский

Процитировано

35

Principles of long-range gene regulation DOI Creative Commons
Sanyami Zunjarrao, Maria Cristina Gambetta

Current Opinion in Genetics & Development, Год журнала: 2025, Номер 91, С. 102323 - 102323

Опубликована: Фев. 13, 2025

Transcription from gene promoters occurs in specific spatiotemporal patterns multicellular organisms, controlled by genomic regulatory elements. The communication between a element and promoter requires certain degree of physical proximity them; hence, most regulation locally the genome. However, recent discoveries have revealed long-range strategies that enhance interactions elements overcoming distances them linear These new findings challenge traditional view how expression are controlled. This review examines recently reported Drosophila mammals, offering insights into their mechanisms evolution.

Язык: Английский

Процитировано

2

Tuning cohesin trajectories enables differential readout of the Pcdhα cluster across neurons DOI
Lea Kiefer, Simon Gaudin, Sandy M. Rajkumar

и другие.

Science, Год журнала: 2024, Номер 385(6707)

Опубликована: Июль 25, 2024

Expression of Protocadherin (Pcdh) genes is critical to the generation neuron identity and wiring nervous system. Pcdhα are arranged in clusters exhibit a range expression profiles, from stochastic deterministic. Because promoters have high sequence share distal enhancers, how distinct neurons choose which gene express remains unclear. We show that interplay between multiple epigenetics, genome folding orchestrates differential readouts locus across neurons. The probability promoter choice depends on enhancer/promoter encounters catalyzed by cohesin, whose extrusion trajectories determine likelihood an individual can "escape" heterochromatin-mediated silencing. propose tunable locus-specific regulatory elements cell type-specific cohesin activity underlie cellular diversity Pcdh genes.

Язык: Английский

Процитировано

9

Genomic snowflakes: how the uniqueness of DNA folding allows us to smell the chemical universe DOI
Longzhi Tan, Xiaoliang Sunney Xie, Stavros Lomvardas

и другие.

Current Opinion in Genetics & Development, Год журнала: 2025, Номер 92, С. 102329 - 102329

Опубликована: Март 18, 2025

Язык: Английский

Процитировано

1

Transcriptional priming and chromatin regulation during stochastic cell fate specification DOI Creative Commons

Alison J. Ordway,

Rina N. Helt,

Robert J. Johnston

и другие.

Philosophical Transactions of the Royal Society B Biological Sciences, Год журнала: 2024, Номер 379(1900)

Опубликована: Март 4, 2024

Stochastic cell fate specification, in which a chooses between two or more fates with set probability, diversifies subtypes development. Although this is vital process across species, common mechanism for these decisions remains elusive. This review examines well-characterized stochastic to identify commonalities their developmental programmes. In the fly eye, of R7 photoreceptors are specified by ON/OFF expression transcription factor, spineless . mouse olfactory system, sensory neurons (OSNs) randomly select express one copy an receptor (OR) gene out pool 2800 alleles. Despite differences systems, both choices rely on dynamic interplay transcriptional priming, chromatin regulation and terminal expression. The coupling modifications primes loci undifferentiated neurons, enabling later during differentiation. Here, we compare mechanisms, examine broader implications development posit key challenges moving forward. article part discussion meeting issue ‘Causes consequences processes disease’.

Язык: Английский

Процитировано

3

Enhancer‐dependent gene regulation in space, time, and malignancies DOI Creative Commons

Beat Blum,

Victoria Dachtler,

Angelika Feldmann

и другие.

International Journal of Cancer, Год журнала: 2025, Номер unknown

Опубликована: Янв. 31, 2025

Control of cell-type-specific gene activation requires the coordinated activity distal regulatory elements, including enhancers, whose inputs must be temporally integrated. Dysregulation this capacity, such as aberrant usage can result in malignant transformation cells. In review, we provide an overview our current understanding enhancer-driven regulation and discuss how may integrated across time, followed by epigenetic structural alterations enhancers cancers.

Язык: Английский

Процитировано

0

Evolution of vertebrate olfactory receptor repertoires and their function DOI Creative Commons
Sigrun I. Korsching

Current Opinion in Behavioral Sciences, Год журнала: 2025, Номер 61, С. 101483 - 101483

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0

3D spatial transcriptomics reveals the molecular domain structure of the mouse olfactory bulb DOI Creative Commons
Nell Klimpert, Mihály Köllő, David H. Brann

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 24, 2025

A core organizing principle of the vertebrate brain is its symmetry along multiple axes. However, structure these axes, and precision with which neurons, circuit modules, regions align to them, remain poorly understood. Here, we used 3D spatial transcriptomics reconstruct anatomical molecular organization mouse olfactory bulb. We mapped positions nearly one thousand molecularly distinct glomeruli, structural functional units odor processing, revealing highly symmetric across hemispheres. Within each bulb, defined a curved axis that divides pairs sister glomeruli. Gene expression programs in sensory neurons predicted glomerular position near-glomerular resolution. was disrupted deeper layer mitral granule cells, suggesting reorganization bulb output pathways. Our findings provide first comprehensive map domain

Язык: Английский

Процитировано

0

Reorganization of the heterochromatin-associated gene-dense subcompartment in early neuronal development DOI Creative Commons

Nicolas J. Scrutton Alvarado,

Ziyu Zhao,

Tomoko Yamada

и другие.

Biology Open, Год журнала: 2025, Номер 14(5)

Опубликована: Май 12, 2025

ABSTRACT The 3D organization of the genome has emerged as an important regulator cellular development. Post-mitotic neurons undergo conserved changes in organization, such inward radial repositioning heterochromatin-rich chromosomes they differentiate. Additionally, transcriptionally active but heterochromatin-associated gene-dense (hGD) regions significantly strengthen their long-distance interactions during cerebellar However, specific developmental stages which these nuclear take place have remained poorly defined. Here, we report that hGD relocalize toward interior and chromosomal immature granule transition from cell migration to subsequent neuronal differentiation. During this period, genomic are coordinately repositioned nucleus alongside physically tethered heterochromatic chromocenters. Despite major subcompartment remains distinct other or repressive bodies, including chromocenters, throughout Notably, appear be independent transcriptional occur neuron Together, our results provide insights into timing structural post-mitotic neurons.

Язык: Английский

Процитировано

0

Solid phase transitions as a solution to the genome folding paradox DOI
Joan Pulupa,

Natalie G. McArthur,

Olga Stathi

и другие.

Nature, Год журнала: 2025, Номер unknown

Опубликована: Май 14, 2025

Язык: Английский

Процитировано

0