Characterization of direct Purkinje cell outputs to the brainstem DOI Creative Commons
Christopher H. Chen, Zhiyi Yao, Shuting Wu

и другие.

eLife, Год журнала: 2024, Номер unknown

Опубликована: Окт. 17, 2024

Purkinje cells (PCs) primarily project to cerebellar nuclei but also directly innervate the brainstem. Some PC-brainstem projections have been described previously, most not thoroughly characterized. Here, we use a PC-specific cre line anatomically and electrophysiologically characterize PC synapses are surprisingly widespread, with highest densities found in vestibular parabrachial nuclei. However, there pronounced regional differences synaptic within both Large optogenetically evoked PC-IPSCs preferentially observed subregions of putative boutons, suggesting that PCs selectively influence these areas behaviors they regulate. Unexpectedly, pontine central gray nearby subnuclei contained low density large small fraction cells. We combined electrophysiological recordings immunohistochemistry assess molecular identities two potential targets: onto mesencephalic trigeminal neurons were even though close proximity boutons; locus coeruleus exceedingly rare or absent, previous studies concluded major input neurons. The availability highly selective for allowed us study functional synapses, while avoiding complications can accompany viral approaches. conclude numerous brainstem nuclei, many strongly inhibit This suggests target cell types specific behavioral roles

Язык: Английский

Cerebellar contributions to dystonia: unraveling the role of Purkinje cells and cerebellar nuclei DOI Creative Commons

Nichelle N. Jackson,

Jacob A. Stagray,

Heather D. Snell

и другие.

Dystonia, Год журнала: 2025, Номер 4

Опубликована: Фев. 17, 2025

Dystonias are a group of neurodegenerative disorders that result in altered physiology associated with motor movements. Both the basal ganglia and cerebellum, brain regions involved learning, sensory perception integration, reward, have been implicated pathology dystonia, but cellular subcellular mechanisms remain diverse for some forms elusive. The goal current review is to summarize recent evidence cerebellar involvement different subtypes dystonia focus on Purkinje cell (PC) nuclei (CN) dysfunction, find commonalities could lay groundwork future development therapeutics patients dystonia. Here we will briefly discuss physical functional connections between cerebellum how these contribute dystonic symptoms. We proceed use human animal model data contributions types specific dystonias movement where secondary symptom. Ultimately, suggest PC CN irregularity be locus through impaired calcium dynamics.

Язык: Английский

Процитировано

1
Characterization of direct Purkinje cell outputs to the brainstem DOI Open Access
Christopher H. Chen, Zhiyi Yao, Shuting Wu

и другие.

Опубликована: Фев. 10, 2025

Purkinje cells (PCs) primarily project to cerebellar nuclei but also directly innervate the brainstem. Some PC-brainstem projections have been described previously, most not thoroughly characterized. Here we use a PC-specific cre line anatomically and electrophysiologically characterize PC synapses are surprisingly widespread, with highest densities found in vestibular parabrachial nuclei. However, there pronounced regional differences synaptic within both Large optogenetically-evoked PC-IPSCs preferentially observed subregions of synapses, suggesting that PCs selectively influence these areas behaviors they regulate. Unexpectedly, pontine central gray nearby subnuclei contained low density large small fraction cells. We combined electrophysiological recordings immunohistochemistry assess molecular identities two putative targets: onto mesencephalic trigeminal neurons were even though close proximity boutons. locus coeruleus exceedingly rare or absent, previous studies concluded major input neurons. The availability highly selective for allowed us study functional while avoiding complications can accompany viral approaches. conclude numerous brainstem nuclei, only inhibit many This suggests target cell types specific behavioral roles regions.

Язык: Английский

Процитировано

0
Characterization of direct Purkinje cell outputs to the brainstem DOI Creative Commons
Christopher H. Chen, Zhiyi Yao, Shuting Wu

и другие.

eLife, Год журнала: 2025, Номер 13

Опубликована: Фев. 27, 2025

Purkinje cells (PCs) primarily project to cerebellar nuclei but also directly innervate the brainstem. Some PC-brainstem projections have been described previously, most not thoroughly characterized. Here, we use a PC-specific cre line anatomically and electrophysiologically characterize PC synapses are surprisingly widespread, with highest densities found in vestibular parabrachial nuclei. However, there pronounced regional differences synaptic within both Large optogenetically evoked PC-IPSCs preferentially observed subregions of putative boutons, suggesting that PCs selectively influence these areas behaviors they regulate. Unexpectedly, pontine central gray nearby subnuclei contained low density large small fraction cells. We combined electrophysiological recordings immunohistochemistry assess molecular identities two potential targets: onto mesencephalic trigeminal neurons were even though close proximity boutons; locus coeruleus exceedingly rare or absent, previous studies concluded major input neurons. The availability highly selective for allowed us study functional synapses, while avoiding complications can accompany viral approaches. conclude numerous brainstem nuclei, many strongly inhibit This suggests target cell types specific behavioral roles

Язык: Английский

Процитировано

0
Anatomy, histology, physiology, and connectivity of the cerebellum DOI

Jos J. Eggermont

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 1 - 19

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Purkinje Cell spike patterns do not correlate with nuclei cell spike patterns in mouse models for cerebellar disease DOI Creative Commons
Aurore Lyon, Meike E. van der Heijden

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Май 21, 2025

Cerebellar dysfunction causes various movement disorders, including ataxia, dystonia, and tremor. Previous work demonstrated that spike patterns in cerebellar nuclei neurons were distinct between different disorder mouse models. However, often these changes arise from neural the cortex, through misfiring, miswiring, or degenerating Purkinje cells. Even though cells form sole output their information is relayed to other regions of motor network via make GABAergic synapses onto cells, it assumed cell result inverse patterns. Here, we test this hypothesis by answering question whether a reliable relationship exists. Single-cell, vivo electrophysiology recordings both types six models for disorders analyzed according parameters relating rate irregularity. We investigated correlated with found some firing irregularity positively but no - particularly was observed rate. Overall, study begins illuminate activity disease state more complex unpredictable. The data suggest cannot accurately predict changes, which ultimately drive states. Our findings underscore importance studying function disease, as lack can mask disease-causing

Язык: Английский

Процитировано

0
A dual Purkinje cell rate and synchrony code sculpts reach kinematics DOI Creative Commons
Abdulraheem Nashef,

Michael S. Spindle,

Dylan Calame

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Июль 12, 2023

Abstract Cerebellar Purkinje cells (PCs) encode movement kinematics in their population firing rates. Firing rate suppression is hypothesized to disinhibit neurons the cerebellar nuclei, promoting adaptive adjustments. Debates persist, however, about whether a second disinhibitory mechanism, PC simple spike synchrony, relevant code. We addressed this question by relating and synchrony patterns recorded with high density probes, mouse reach kinematics. discovered behavioral correlates of that align known causal relationship between activity output. Reach deceleration was positively correlated both decreases consistent mechanisms disinhibiting target neurons, which are adjust velocity. Direct tests contribution each coding scheme nuclear using dynamic clamp, combining physiological ex vivo, confirmed levels highly facilitatory for firing. These findings suggest collaborate exert fine control movement.

Язык: Английский

Процитировано

4
The cerebellar nuclei take center stage DOI
Dana Cohen

Current Opinion in Behavioral Sciences, Год журнала: 2024, Номер 57, С. 101362 - 101362

Опубликована: Март 23, 2024

Язык: Английский

Процитировано

1
Characterization of direct Purkinje cell outputs to the brainstem DOI Creative Commons
Christopher H. Chen, Zhiyi Yao, Shuting Wu

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 18, 2024

Abstract Purkinje cells (PCs) primarily project to cerebellar nuclei but also directly innervate the brainstem. Some PC-brainstem projections have been described previously, most not thoroughly characterized. Here we use a PC-specific cre line anatomically and electrophysiologically characterize PC synapses are surprisingly widespread, with highest densities found in vestibular parabrachial nuclei. However, there pronounced regional differences synaptic within both Large optogenetically-evoked PC-IPSCs preferentially observed subregions of synapses, suggesting that PCs selectively influence these areas behaviors they regulate. Unexpectedly, pontine central gray nearby subnuclei contained low density large small fraction cells. We combined electrophysiological recordings immunohistochemistry assess molecular identities two putative targets: onto mesencephalic trigeminal neurons were even though close proximity boutons. locus coeruleus exceedingly rare or absent, previous studies concluded major input neurons. The availability highly selective for allowed us study functional while avoiding complications can accompany viral approaches. conclude numerous brainstem nuclei, only inhibit many This suggests target cell types specific behavioral roles regions.

Язык: Английский

Процитировано

0
Characterization of direct Purkinje cell outputs to the brainstem DOI Open Access
Christopher H. Chen, Zhiyi Yao, Shuting Wu

и другие.

Опубликована: Окт. 17, 2024

Purkinje cells (PCs) primarily project to cerebellar nuclei but also directly innervate the brainstem. Some PC-brainstem projections have been described previously, most not thoroughly characterized. Here we use a PC-specific cre line anatomically and electrophysiologically characterize PC synapses are surprisingly widespread, with highest densities found in vestibular parabrachial nuclei. However, there pronounced regional differences synaptic within both Large optogenetically-evoked PC-IPSCs preferentially observed subregions of synapses, suggesting that PCs selectively influence these areas behaviors they regulate. Unexpectedly, pontine central gray nearby subnuclei contained low density large small fraction cells. We combined electrophysiological recordings immunohistochemistry assess molecular identities targets. onto mesencephalic trigeminal neurons were even though close proximity boutons. locus coeruleus exceedingly rare or absent, previous studies concluded major input neurons. The availability highly selective for allowed us study functional while avoiding complications can accompany viral approaches. conclude numerous brainstem nuclei, only inhibit many This suggests target cell types specific behavioral roles regions.

Язык: Английский

Процитировано

0
Characterization of direct Purkinje cell outputs to the brainstem DOI Creative Commons
Christopher H. Chen, Zhiyi Yao, Shuting Wu

и другие.

eLife, Год журнала: 2024, Номер unknown

Опубликована: Окт. 17, 2024

Purkinje cells (PCs) primarily project to cerebellar nuclei but also directly innervate the brainstem. Some PC-brainstem projections have been described previously, most not thoroughly characterized. Here, we use a PC-specific cre line anatomically and electrophysiologically characterize PC synapses are surprisingly widespread, with highest densities found in vestibular parabrachial nuclei. However, there pronounced regional differences synaptic within both Large optogenetically evoked PC-IPSCs preferentially observed subregions of putative boutons, suggesting that PCs selectively influence these areas behaviors they regulate. Unexpectedly, pontine central gray nearby subnuclei contained low density large small fraction cells. We combined electrophysiological recordings immunohistochemistry assess molecular identities two potential targets: onto mesencephalic trigeminal neurons were even though close proximity boutons; locus coeruleus exceedingly rare or absent, previous studies concluded major input neurons. The availability highly selective for allowed us study functional synapses, while avoiding complications can accompany viral approaches. conclude numerous brainstem nuclei, many strongly inhibit This suggests target cell types specific behavioral roles

Язык: Английский

Процитировано

0