Structure-guided secretome analysis of gall-forming microbes offers insights into effector diversity and evolution DOI Creative Commons
Soham Mukhopadhyay, Muhammad Asim Javed,

Jiaxu Wu

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 3, 2024

ABSTRACT Phytopathogens secrete effector molecules to manipulate host immunity and metabolism. Recent advances in structural genomics have identified fungal families whose members adopt similar folds despite sequence divergence, highlighting their importance virulence immune evasion. To extend the scope of comparative structure-guided analysis more evolutionarily distant phytopathogens with lifestyle, we used AlphaFold2 predict 3D structures secretome from selected plasmodiophorid, oomycete, gall-forming pathogens. Clustering protein based on homology revealed species-specific expansions a low abundance known orphan families. We nucleoside hydrolase-like fold, structurally homologous bacterial HopQ1, be conserved among various microbes. also novel sequence-unrelated but (SUSS) clusters, rich motifs. Notably, ankyrin proteins were significantly expanded plasmodiophorids, AlphaFold-Multimer screening revealing MAP kinase pathway as major target these proteins. Altogether, this study our understanding landscapes microbes provides valuable resource for broadening phylogenomic studies across diverse phytopathogens.

Язык: Английский

Structure-guided secretome analysis of gall-forming microbes offers insights into effector diversity and evolution DOI Creative Commons
Soham Mukhopadhyay, Muhammad Asim Javed,

Jiaxu Wu

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 3, 2024

ABSTRACT Phytopathogens secrete effector molecules to manipulate host immunity and metabolism. Recent advances in structural genomics have identified fungal families whose members adopt similar folds despite sequence divergence, highlighting their importance virulence immune evasion. To extend the scope of comparative structure-guided analysis more evolutionarily distant phytopathogens with lifestyle, we used AlphaFold2 predict 3D structures secretome from selected plasmodiophorid, oomycete, gall-forming pathogens. Clustering protein based on homology revealed species-specific expansions a low abundance known orphan families. We nucleoside hydrolase-like fold, structurally homologous bacterial HopQ1, be conserved among various microbes. also novel sequence-unrelated but (SUSS) clusters, rich motifs. Notably, ankyrin proteins were significantly expanded plasmodiophorids, AlphaFold-Multimer screening revealing MAP kinase pathway as major target these proteins. Altogether, this study our understanding landscapes microbes provides valuable resource for broadening phylogenomic studies across diverse phytopathogens.

Язык: Английский

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