Innervation density governs crosstalk of GPCR-based norepinephrine and dopamine sensors DOI Creative Commons
Ruth Palan Lopez,

Natalie Noble,

Özge Demet Özçete

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 23, 2024

Summary GPCR-based fluorescent sensors are widely used to correlate neuromodulatory signaling with brain function. While experiments in transfected cells often reveal selectivity for individual neurotransmitters, sensor specificity the frequently remains uncertain. Pursuing slices and vivo, we find that norepinephrine dopamine cross-activate respective sensors. Non-specific activation occurred when innervation of cross-reacting transmitter was high, silencing specific indispensable interpreting fluorescence.

Язык: Английский

Dopamine Drives Feedforward Inhibition to Orexin Feeding System, Mediating Weight Loss Induced by Morphine Addiction DOI Creative Commons
Huiming Li, Sa Wang, Dan Wang

и другие.

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Янв. 21, 2025

Abstract Feeding behavior changes induced by opioid addiction significantly contribute to the worsening crisis. Activation of reward system has shown provoke binge eating disorder in individuals with use disorder, whereas prolonged exposure leads weight loss. Understanding mechanisms underlying these phenomena is essential for addressing this pressing societal issue. This study demonstrates that loss resulting from feeding during morphine requires activation ventral tegmental area dopamine (DA) system, which suppresses orexin center. Specifically, DA exerts an inhibitory effect on neurons lateral hypothalamus (LHA) through a feedforward inhibition mediated GABA LHA, involving D1 receptors (D1R) and T‐type Ca 2+ channels. Moreover, addiction‐induced reduction body food intake can be reversed D1R antagonist SCH23390 chemogenetic silencing LHA. These findings delineate neuromodulatory mechanism addiction‐associated

Язык: Английский

Процитировано

0

Abundance of dopamine and its receptors in the brain and adipose tissue following diet-induced obesity or caloric restriction DOI Creative Commons

Fleur W Hukema,

Susanne Hetty, Christakis Kagios

и другие.

Translational research, Год журнала: 2025, Номер 280, С. 41 - 54

Опубликована: Май 8, 2025

While obesity and type 2 diabetes (T2D) are associated with altered dopaminergic activity in the central nervous system adipose tissue (AT), directions underlying mechanisms remain inconclusive. Therefore, we characterized changes abundance of dopamine, its metabolites, receptors DRD1 DRD2 brain AT upon dietary intervention or obesity. Male Wistar rats were fed either a standard pellet diet, cafeteria diet inducing insulin resistance, calorie-restricted for 12 weeks. Abundance dopamine examined regions relevant feeding behavior energy homeostasis. Furthermore, protein levels analyzed rat inguinal epidydimal human subcutaneous omental from individuals without Rats diet-induced displayed higher levels, as well receptor caudate putamen nucleus accumbens core. Surprisingly, caloric restriction induced similar DRD2, but not brain. Both diets reduced AT, upregulated AT. obesity, elevated only while was both These findings highlight responses to balance, occurring We propose that pathways involved crosstalk during development T2D.

Язык: Английский

Процитировано

0

Innervation density governs crosstalk of GPCR-based norepinephrine and dopamine sensors DOI Creative Commons
Ruth Palan Lopez,

Natalie Noble,

Özge Demet Özçete

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 23, 2024

Summary GPCR-based fluorescent sensors are widely used to correlate neuromodulatory signaling with brain function. While experiments in transfected cells often reveal selectivity for individual neurotransmitters, sensor specificity the frequently remains uncertain. Pursuing slices and vivo, we find that norepinephrine dopamine cross-activate respective sensors. Non-specific activation occurred when innervation of cross-reacting transmitter was high, silencing specific indispensable interpreting fluorescence.

Язык: Английский

Процитировано

0