Excitation/Inhibition balance relates to cognitive function and gene expression in temporal lobe epilepsy: a high density EEG assessment with aperiodic exponent
Brain Communications,
Год журнала:
2024,
Номер
6(4)
Опубликована: Янв. 1, 2024
Patients
with
epilepsy
are
characterized
by
a
dysregulation
of
excitation/inhibition
balance
(E/I).
The
assessment
E/I
may
inform
clinicians
during
the
diagnosis
and
therapy
management,
even
though
it
is
rarely
performed.
An
accessible
measure
brain
represents
clinically
relevant
feature.
Here,
we
exploited
exponent
aperiodic
component
power
spectrum
electroencephalography
(EEG)
signal,
as
non-invasive
cost-effective
proxy
balance.
We
recorded
resting-state
activity
high-density
EEG
from
67
patients
temporal
lobe
35
controls.
extracted
fit
source-reconstructed
tested
differences
between
Spearman's
correlation
was
performed
clinical
variables
(age
onset,
duration
neuropsychology)
cortical
expression
epilepsy-related
genes
derived
Allen
Human
Brain
Atlas.
showed
significantly
larger
exponent,
corresponding
to
inhibition-directed
balance,
in
bilateral
frontal
regions.
Lower
left
entorhinal
dorsolateral
prefrontal
cortices
corresponded
lower
performance
short-term
verbal
memory.
Limited
epilepsy,
detected
significant
Язык: Английский
Serotonin acts through multiple cellular targets during an olfactory critical period.
iScience,
Год журнала:
2024,
Номер
27(11), С. 111083 - 111083
Опубликована: Окт. 9, 2024
Язык: Английский
BK channel gain-of-function disrupts limb control by suppressing neurotransmission during a critical developmental window
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Сен. 22, 2023
SUMMARY
Gain-of-function
mutations
in
BK
potassium
channels
(BK
GOF)
cause
debilitating
involuntary
limb
movements.
modulate
action
potential
shape
and
neurotransmission
mature
neurons,
yet
some
GOF
also
neurodevelopmental
morbidities.
Thus,
whether
impairs
control
by
altering
the
excitation/inhibition
of
motor
circuits,
or
disrupting
their
development,
remains
unclear.
To
address
this
issue,
we
developed
a
genetic
method
enabling
spatiotemporal
channel
expression
neurons
fruit
fly,
Drosophila
.
In
concert
with
high-resolution
measurements
kinematics,
demonstrate
that
act
during
narrow
period
to
perturb
adult
flies.
During
period,
alters
synaptic
localisation
key
active
zone
protein
Bruchpilot
suppresses
excitatory
neurotransmission.
wild-type
background,
find
reducing
neural
activity
neurodevelopment
yields
similar
defects
those
observed
Conversely,
enhancing
excitability
development
rescues
alterations
kinematics
Collectively,
our
results
suggest
perturbs
largely
activity-dependent
aspects
neuronal
development.
Язык: Английский
Active microrheology with a single, time-shared laser trap
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Окт. 20, 2023
Abstract
Recording
the
mechanical
response
of
biological
samples,
cell’s
interior
and
complex
fluids
in
general,
would
enable
deeper
understanding
cellular
differentiation,
ageing
drug
discovery.
Here,
we
present
a
time-shared
optical
tweezer
microrheology
(TimSOM)
pipeline
to
determine
frequency-
age-dependent
viscoelastic
properties
materials.
Our
approach
consists
splitting
single
laser
beam
into
two
near-instantaneous
traps
carry
out
simultaneous
force
displacement
measurements
with
sub-nanometer
sub-picoNewton
accuracy
during
sinusoidal
perturbations.
Leveraging
numerical
analytical
models,
find
solutions
commonly
encountered
deviations,
build
practical
robust
nanorheometer.
We
demonstrate
versatility
technique
by
1)
measuring
phase
transitions
an
biomolecular
condensate,
2)
quantifying
three
intracellular
compartments
zebrafish
progenitor
cells,
and,
3)
using
Caenorhabditis
elegans
,
uncover
how
mutations
causing
nuclear
envelopathies
soften
cytosol
intestinal
cells
organismal
age.
Together,
our
advances
afford
rapid
phenotyping
material
inside
proteins
blends,
opening
avenues
for
biomedical
screening
applications.
Язык: Английский
Circadian control in the timing of critical periods duringDrosophilalarval neuronal development.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Март 25, 2024
Abstract
Critical
periods
(CPs)
of
development
are
temporal
windows
heightened
neural
plasticity.
Activity
perturbation
during
CPs
can
produce
significant,
and
permanent,
alterations
to
the
circuits.
In
this
study
we
report
a
circadian
mechanism
underlying
timing
in
Drosophila
embryonic
larval
development.
These
occur
at
∼24
hr
intervals
open
manipulation
through
blue
light
(BL)-activation
regulator
Cryptochrome
(CRY).
This
is
sufficient
destabilize
CNS,
evidenced
by
an
induced
seizure
phenotype
when
tested
third
instar
(L3).
addition
CRY
nulls,
genetic
ablation
period
gene
also
mitigates
BL
exposure
and,
moreover,
alleles
that
affect
alter
CPs.
Our
analysis
shows
clear
role
for
main
clock
neuropeptide,
pigment
dispersing
factor
(PDF),
transduce
output
these
Targeted
PDF
receptor
knockdown,
either
GABAergic
or
CRY-positive
neurons,
prevent
CRY-mediated
phenotype.
first
demonstration
larvae,
whilst
alone
major
significance,
our
results
highlight
potential
using
larvae
as
model
investigate
impact
rhythms
on
early
neuronal
higher
organisms,
which
remains
experimentally
challenging.
Significance
Statement
Whilst
biological
well
understood
adult
same
not
true
How
maternal
impacts
mammalian
fetus
poorly
understood.
Given
many
expectant
mothers
experience
altered
rhythms,
largely
due
nightshift
working,
it
important
address
concerns.
Here
identify
clock-mediated
be
manipulated
light.
findings
provide
experimental
opportunity
better
understand
Язык: Английский
Serotonin acts through multiple cellular targets during an olfactory critical period.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Апрель 14, 2024
ABSTRACT
Serotonin
(5-HT)
is
known
to
modulate
early
development
during
critical
periods
when
experience
drives
heightened
levels
of
plasticity
in
neurons.
Here,
we
take
advantage
the
genetically
tractable
olfactory
system
Drosophila
investigate
how
5-HT
modulates
period
CO2
sensing
circuit
fruit
flies.
Our
study
reveals
that
5HT
modulation
multiple
neuronal
targets
necessary
for
experience-dependent
structural
changes
an
odor
processing
circuit.
The
CPP
involve
local
inhibitory
networks
and
consistent
with
this
found
knocking
down
5-HT7
receptors
a
subset
GABAergic
interneurons
was
sufficient
block
CPP,
as
GABA
expressed
by
sensory
neurons
(OSNs).
Additionally,
direct
OSNs
via
5-HT2B
expression
cognate
also
essential
CPP.
Furthermore,
5-HT1B
serotonergic
required
period.
Язык: Английский
OptoChamber: A Low-cost, Easy-to-Make, Customizable, and Multi-Chambered Electronic Device for Applying Optogenetic Stimulation to Larval
PubMed,
Год журнала:
2024,
Номер
2024
Опубликована: Янв. 1, 2024
Optogenetics
is
a
powerful
tool
used
to
manipulate
physiological
processes
in
animals
through
cell-specific
expression
of
genetically
modified
channelrhodopsins.
In
Язык: Английский
Heterogeneous responses to embryonic critical period perturbations among different components of theDrosophilalarval locomotor circuit
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 16, 2024
Abstract
As
developing
neural
circuits
become
functional,
they
undergo
a
phase
of
heightened
plasticity
in
response
to
intrinsic
and/or
extrinsic
stimuli.
These
developmental
windows
are
termed
critical
periods
(CPs),
because
perturbations
during
the
CP
can
lead
lasting
and
significant
change
subsequent
development,
such
as
sub-optimal
unstable
networks.
By
contrast,
same
manipulations
before
or
after
does
not
create
changes.
Here,
we
have
used
Drosophila
larval
locomotor
network
study
how
different
identified,
connected
elements
respond
perturbation,
from
pre-motor
interneuron
motoneuron,
neuromuscular
junction.
Using
heat
stress
an
ecologically
relevant
stimulus,
show
that
increasing
temperature
causes
increased
activity
that,
when
applied
CP,
leads
larvae
crawl
more
slowly
require
longer
recover
electroshock-induced
seizures,
indicative
decreased
stability.
Within
central
nervous
system,
find
perturbation
interneurons
delivering
synaptic
drive
motoneurons,
which
turn
display
reduced
excitability.
The
peripheral
junction,
on
other
hand,
maintains
normal
transmission,
despite
structural
changes
terminal
overgrowth
altered
postsynaptic
receptor
field
composition.
Overall,
our
data
demonstrate
within
differentially
perturbation.
Our
results
suggest
underlying
sequence,
hierarchy,
adjustment
CPs,
present
highly
tractable
experimental
model
system
with
biology.
Язык: Английский