In vivo autofluorescence lifetime imaging of the Drosophila brain captures metabolic shifts associated with memory formation

Опубликована: Март 31, 2025

Neuronal energy regulation is increasingly recognized as a critical factor underlying brain functions and their pathological alterations, yet the metabolic dynamics that accompany cognitive processes remain poorly understood. As label-free minimally invasive technique, fluorescence lifetime imaging (FLIM) of coenzymes NADH NADPH (collectively referred to NAD(P)H) offers possibility resolve cellular profiles with high spatial precision. However, NAD(P)H FLIM’s capacity detect subtle changes in neuronal metabolism associated cognition has not been demonstrated. In this study, we applied FLIM map Drosophila neurons vivo across multiple scales, focusing on primary centers for associative memory: mushroom bodies (MBs). At broad scale, obtained an overview signatures main tissue identified marked difference between neuropil cortex areas. finer our findings revealed notable heterogeneity basal distinct MB neuron subtypes. Measurements performed after olfactory learning also uncovered subtype-specific shift memory formation, demonstrating utility detecting physiology-driven linked function. These results establish promising framework studying cerebral vivo.

Язык: Английский

In vivo autofluorescence lifetime imaging of the Drosophila brain captures metabolic shifts associated with memory formation

Опубликована: Март 31, 2025

Neuronal energy regulation is increasingly recognized as a critical factor underlying brain functions and their pathological alterations, yet the metabolic dynamics that accompany cognitive processes remain poorly understood. As label-free minimally invasive technique, fluorescence lifetime imaging (FLIM) of coenzymes NADH NADPH (collectively referred to NAD(P)H) offers possibility resolve cellular profiles with high spatial precision. However, NAD(P)H FLIM’s capacity detect subtle changes in neuronal metabolism associated cognition has not been demonstrated. In this study, we applied FLIM map Drosophila neurons vivo across multiple scales, focusing on primary centers for associative memory: mushroom bodies (MBs). At broad scale, obtained an overview signatures main tissue identified marked difference between neuropil cortex areas. finer our findings revealed notable heterogeneity basal distinct MB neuron subtypes. Measurements performed after olfactory learning also uncovered subtype-specific shift memory formation, demonstrating utility detecting physiology-driven linked function. These results establish promising framework studying cerebral vivo.

Язык: Английский

Diverting glial glycolytic flux towards neurons is a memory-relevant role of Drosophila CRH-like signalling

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Дек. 2, 2024

Abstract An essential role of glial cells is to comply with the large and fluctuating energy needs neurons. Metabolic adaptation integral acute stress response, suggesting that could be major, yet overlooked, targets hormones. Here we show Dh44 neuropeptide, Drosophila homologue mammalian corticotropin-releasing hormone (CRH), acts as an experience-dependent metabolic switch for glycolytic output in glia. released by dopamine neurons limits fatty acid synthesis build-up lipid stores. Although basally active, this hormonal axis acutely stimulated following learning a danger-predictive cue. This results transient suppression anabolic use pyruvate, sparing it memory-relevant supply Diverting pyruvate destination may dampen need upregulate glycolysis response increased neuronal demand. beneficial efficiency memory formation, mechanism reveals ongoing competition between fuelling anabolism.

Язык: Английский