Starvation-induced phosphorylation activates gasdermin A to initiate pyroptosis DOI Creative Commons
Xinran Li, Wei Li, Cong Xiang

и другие.

Cell Reports, Год журнала: 2024, Номер 43(9), С. 114728 - 114728

Опубликована: Сен. 1, 2024

Pyroptosis, a pro-inflammatory form of programmed cell death, is crucial for host defense against pathogens and danger signals. Proteolytic cleavage gasdermin proteins B-E (GSDMB-GSDME) well established as trigger pyroptosis, but the intracellular activation mechanism GSDMA remains elusive. Here, we demonstrate that severe starvation induces pyroptosis through phosphorylation-induced GSDMA. Nutrient stresses stimulate via phosphorylation mediated by Unc-51-like autophagy-activating kinase 1 (ULK1). Phosphorylation Ser353 on human ULK1 or phospho-mimetic Ser353Asp mutant liberates from auto-inhibition, facilitating its membrane targeting initiation pyroptosis. To further validate significance phosphorylation, generated constitutively active Ser354Asp mouse Gsdma, which induced skin inflammation hyperplasia in mice, reminiscent phenotypes with activated Gsdma. This study uncovers underlying cellular response to nutrient stress.

Язык: Английский

The gasdermin family: emerging therapeutic targets in diseases DOI Creative Commons
Cheng‐long Zhu, Sheng Xu, Ruoyu Jiang

и другие.

Signal Transduction and Targeted Therapy, Год журнала: 2024, Номер 9(1)

Опубликована: Апрель 7, 2024

Abstract The gasdermin (GSDM) family has garnered significant attention for its pivotal role in immunity and disease as a key player pyroptosis. This recently characterized class of pore-forming effector proteins is orchestrating processes such membrane permeabilization, pyroptosis, the follow-up inflammatory response, which are crucial self-defense mechanisms against irritants infections. GSDMs have been implicated range diseases including, but not limited to, sepsis, viral infections, cancer, either through involvement pyroptosis or independently this process. regulation GSDM-mediated gaining recognition promising therapeutic strategy treatment various diseases. Current strategies inhibiting GSDMD primarily involve binding to GSDMD, blocking cleavage GSDMD-N-terminal (NT) oligomerization, albeit with some off-target effects. In review, we delve into cutting-edge understanding interplay between elucidate activation GSDMs, explore their associations diseases, discuss recent advancements potential developing inhibitors.

Язык: Английский

Процитировано

26

Gasdermins as evolutionarily conserved executors of inflammation and cell death DOI
Kaiwen W. Chen, Petr Brož

Nature Cell Biology, Год журнала: 2024, Номер 26(9), С. 1394 - 1406

Опубликована: Авг. 26, 2024

Язык: Английский

Процитировано

15

Mechanistic insights into gasdermin-mediated pyroptosis DOI
Yang Bai,

Youdong Pan,

Xing Liu

и другие.

Nature Reviews Molecular Cell Biology, Год журнала: 2025, Номер unknown

Опубликована: Март 24, 2025

Язык: Английский

Процитировано

1

Harnessing Pyroptosis for Cancer Immunotherapy DOI Creative Commons
Christopher M. Bourne, Cornelius Y. Taabazuing

Cells, Год журнала: 2024, Номер 13(4), С. 346 - 346

Опубликована: Фев. 16, 2024

Cancer immunotherapy is a novel pillar of cancer treatment that harnesses the immune system to fight tumors and generally results in robust antitumor immunity. Although has achieved remarkable clinical success for some patients, many patients do not respond, underscoring need develop new strategies promote Pyroptosis an immunostimulatory type regulated cell death activates innate system. A hallmark pyroptosis release intracellular contents such as cytokines, alarmins, chemokines can stimulate adaptive activation. Recent studies suggest promotes Here, we review mechanisms by which be induced highlight induce cells defense. We discuss how modulates tumor microenvironment immunity also research areas focus on continued development anticancer treatment. Pyroptosis-based therapies offer promising avenue treating immunologically 'cold' tumors.

Язык: Английский

Процитировано

8

Unanticipated Loss of Inflammasomes in Birds DOI Creative Commons
Zachary P. Billman, Dustin C. Hancks,

Edward A Miao

и другие.

Genome Biology and Evolution, Год журнала: 2024, Номер 16(7)

Опубликована: Июль 1, 2024

Abstract Inflammasomes are multiprotein complexes that form in response to ligands originating from pathogens as well alterations of normal cell physiology caused by infection or tissue damage. These structures engage a robust inflammatory immune eradicates environmental microbes before they cause disease, and slow the growth bona fide pathogens. Despite their undeniable utility immunity, inflammasomes radically reduced birds. Perhaps most surprising is that, within all birds, NLRP3 retained, while its signaling adapter ASC lost, suggesting signals via novel unknown adapter. Crocodilian reptiles turtles, which share more recent common ancestor with retain many lost inflammasome components, indicating deletion occurred after birds diverged crocodiles. Some bird lineages have even extensive loss, songbirds continuing pare down until only CARD8 remain. Remarkably, caspase-1 but downstream targets caspase-1: IL-1β, IL-18, YVAD-linker encoding gasdermin A. This suggests can signal through alternative proteases activate cytokine maturation pyroptosis songbirds. observations may reveal new contexts activation be relevant mammalian suggest avenues research uncover enigmatic nature poorly understood inflammasome.

Язык: Английский

Процитировано

3

Caspase 3 proteolytically inactivates gasdermin Ec and inhibits pyroptosis in an early vertebrate DOI Creative Commons
Xin Tian, Shuai Jiang

Comparative Immunology Reports, Год журнала: 2025, Номер unknown, С. 200216 - 200216

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0

Pyroptosis: molecular mechanisms and roles in disease DOI Creative Commons
Petr Brož

Cell Research, Год журнала: 2025, Номер unknown

Опубликована: Апрель 3, 2025

Язык: Английский

Процитировано

0

Pyroptosis: Induction and inhibition strategies for immunotherapy of diseases DOI Creative Commons
Junjun Wu, Hong Wang, Pu Gao

и другие.

Acta Pharmaceutica Sinica B, Год журнала: 2024, Номер 14(10), С. 4195 - 4227

Опубликована: Июнь 28, 2024

Cell death is a central process for organismal health. Pyroptosis, namely pyroptotic cell death, recognized as critical type that disrupts membrane and triggers pro-inflammatory cytokine secretion

Язык: Английский

Процитировано

2

Starvation-induced phosphorylation activates gasdermin A to initiate pyroptosis DOI Creative Commons
Xinran Li, Wei Li, Cong Xiang

и другие.

Cell Reports, Год журнала: 2024, Номер 43(9), С. 114728 - 114728

Опубликована: Сен. 1, 2024

Pyroptosis, a pro-inflammatory form of programmed cell death, is crucial for host defense against pathogens and danger signals. Proteolytic cleavage gasdermin proteins B-E (GSDMB-GSDME) well established as trigger pyroptosis, but the intracellular activation mechanism GSDMA remains elusive. Here, we demonstrate that severe starvation induces pyroptosis through phosphorylation-induced GSDMA. Nutrient stresses stimulate via phosphorylation mediated by Unc-51-like autophagy-activating kinase 1 (ULK1). Phosphorylation Ser353 on human ULK1 or phospho-mimetic Ser353Asp mutant liberates from auto-inhibition, facilitating its membrane targeting initiation pyroptosis. To further validate significance phosphorylation, generated constitutively active Ser354Asp mouse Gsdma, which induced skin inflammation hyperplasia in mice, reminiscent phenotypes with activated Gsdma. This study uncovers underlying cellular response to nutrient stress.

Язык: Английский

Процитировано

2