Emergent 3D genome reorganization from the stepwise assembly of transcriptional condensates

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 27, 2025

Transcriptional condensates are clusters of transcription factors, coactivators, and RNA Pol II associated with high-level gene expression, yet how they assemble function within the cell remains unclear. Here we show that transcriptional form in a stepwise manner to enable both graded three-dimensional (3D) control yeast heat shock response. Molecular dissection revealed condensate cascade. First, factor Hsf1 upon partial dissociation from chaperone Hsp70. Next, coactivator Mediator partitions following further Hsp70 phosphorylation. Finally, condenses, driving emergent coalescence HSR genes. analysis series mutants graded, rather than switch-like, activity. Separation-of-function showed formation can be decoupled activation. Instead, fully assembled promote adaptive 3D genome reconfiguration, suggesting role beyond

Язык: Английский

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Back to the basics: the molecular blueprint of plant heat stress transcription factors

Biological Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 14, 2025

Abstract Heat stress transcription factors (HSFs) play a pivotal role in regulating plant responses to heat and other environmental stresses, as well developmental processes. HSFs possess conserved domains responsible for DNA binding, oligomerization, transcriptional regulation, which collectively enable precise dynamic control of cellular stimuli. Functional diversification has been demonstrated through genetic studies model plants such Arabidopsis thaliana economically important crops like tomato, rice, wheat. However, the underlying molecular mechanisms that govern HSF function remain only partially understood, handful HSFs. Advancements structural biology, biochemistry, genomics shed light into how mediate at level. These insights offer exciting opportunities leverage biology gene editing crop improvement, enabling customization tolerance traits via regulation HSF-dependent regulatory networks enhance thermotolerance. This review synthesizes current knowledge on structure function, providing perspective their roles adaptation changing climate.

Язык: Английский

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Fine-tuning stress responses by auxiliary feedback loops that sense damage repair

The Journal of Cell Biology, Год журнала: 2024, Номер 223(12)

Опубликована: Ноя. 15, 2024

Mogk and den Brave discuss exciting results from a comprehensive screen of heat shock response components in yeast, published this issue by Pincus colleagues (https://doi.org/10.1083/jcb.202401082). Their work reveals modulatory regulatory loops that fine-tune the timing shutdown highly conserved pathway.

Язык: Английский

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