A reaction network model of microscale liquid–liquid phase separation reveals effects of spatial dimension DOI

J.S. Kim,

Sean D. Lawley, Jinsu Kim

и другие.

The Journal of Chemical Physics, Год журнала: 2024, Номер 161(20)

Опубликована: Ноя. 27, 2024

Proteins can form droplets via liquid-liquid phase separation (LLPS) in cells. Recent experiments demonstrate that LLPS is qualitatively different on two-dimensional (2D) surfaces compared to three-dimensional (3D) solutions. In this paper, we use mathematical modeling investigate the causes of discrepancies between 2D and 3D. We model number proteins inducing by continuous-time Markov chains chemical reaction network theory analyze model. To reflect influence space dimension, droplet formation dissociation rates are determined using first hitting times diffusing proteins. show our stochastic reproduces appropriate diagram consistent with relevant thermodynamic constraints. After further analyzing model, find it predicts dimension induces features LLPS, which recent experiments. While has been claimed differences 3D stem mainly from diffusion coefficients, analysis independent coefficients since stationary behavior. Our results thus give new hypotheses about how affects LLPS.

Язык: Английский

Phase behavior of Cacio e Pepe sauce DOI Creative Commons
Giacomo Bartolucci, Daniel Maria Busiello,

Matteo Ciarchi

и другие.

Physics of Fluids, Год журнала: 2025, Номер 37(4)

Опубликована: Апрель 1, 2025

“Pasta alla Cacio e pepe” is a traditional Italian dish made with pasta, pecorino cheese, and pepper. Despite its simple ingredient list, achieving the perfect texture creaminess of sauce can be challenging. In this study, we systematically explore phase behavior pepe sauce, focusing on stability at increasing temperatures for various proportions water, starch. We identify starch concentration as key factor influencing stability, direct implications practical cooking. Specifically, delineate regime where concentrations below 1% (relative to cheese mass) lead formation system-wide clumps, condition determining what term “Mozzarella Phase” corresponding an unpleasant separated sauce. Additionally, examine impact relative water fixed level, observing lower critical solution temperature that theoretically rationalized by means minimal effective free-energy model. further analyze effect less stabilizer, trisodium citrate, observe sharp transition from Mozzarella Phase completely smooth stable in contrast starch-stabilized mixtures, more gradual. Finally, present scientifically optimized recipe based our findings, enabling consistently flawless execution classic dish.

Язык: Английский

Процитировано

0

A reaction network model of microscale liquid–liquid phase separation reveals effects of spatial dimension DOI

J.S. Kim,

Sean D. Lawley, Jinsu Kim

и другие.

The Journal of Chemical Physics, Год журнала: 2024, Номер 161(20)

Опубликована: Ноя. 27, 2024

Proteins can form droplets via liquid-liquid phase separation (LLPS) in cells. Recent experiments demonstrate that LLPS is qualitatively different on two-dimensional (2D) surfaces compared to three-dimensional (3D) solutions. In this paper, we use mathematical modeling investigate the causes of discrepancies between 2D and 3D. We model number proteins inducing by continuous-time Markov chains chemical reaction network theory analyze model. To reflect influence space dimension, droplet formation dissociation rates are determined using first hitting times diffusing proteins. show our stochastic reproduces appropriate diagram consistent with relevant thermodynamic constraints. After further analyzing model, find it predicts dimension induces features LLPS, which recent experiments. While has been claimed differences 3D stem mainly from diffusion coefficients, analysis independent coefficients since stationary behavior. Our results thus give new hypotheses about how affects LLPS.

Язык: Английский

Процитировано

0