Neural substrates of cold nociception in Drosophila larva

Опубликована: Апрель 29, 2025

Metazoans detect and differentiate between innocuous (non-painful) and/or noxious (harmful) environmental cues using primary sensory neurons, which serve as the first node in a neural network that computes stimulus specific behaviors to either navigate away from injury- causing conditions or perform protective mitigate extensive injury. The ability of an animal respond various stimuli depends upon molecular diversity sensors underlying circuitry responsible for relevant behavioral action selection. Recent studies Drosophila larvae have revealed somatosensory class III multidendritic (CIII md) neurons function multimodal regulating distinct responses mechanical nociceptive thermal stimuli. advances circuit bases behavior identified functionally validated larval involved (mechanical) (heat mechanical) cues. However, central processing cold remained unexplored. We implicate multisensory integrators (Basins), premotor (Down-and-Back) projection (A09e TePns) substrates required cold-evoked calcium responses. Neural silencing cell types downstream CIII md led significant reductions co-activation plus additional facilitated contraction (CT) Further, we demonstrate optogenetic activation evokes increases these neurons. Finally, characterize motor neuron CT delineate muscular basis response. Collectively, how process through diverse generating stimulus-specific behaviors.

Язык: Английский

Inhibitory circuits coordinate leg movements duringDrosophilagrooming

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 6, 2024

Limbs execute diverse actions coordinated by the nervous system through multiple motor programs. The basic architecture of neurons that activate muscles articulate joints for antagonistic flexion and extension movements is conserved from flies to vertebrates. While excitatory premotor circuits are expected establish sets leg work together, our study uncovered a new instructive role inhibitory circuits: their ability generate rhythmic movements. Using electron microscopy data Drosophila nerve cord, we categorized ~120 GABAergic 13A 13B hemi-lineages into classes based on similarities in morphology connectivity. By mapping synaptic partners, pathways inhibiting specific groups neurons, disinhibiting counterparts, inducing alternation between extension. We tested function optogenetic activation silencing, using an in-depth ethological analysis during grooming. combined anatomy behavior findings construct computational model can reproduce major aspects observed behavior, confirming sufficiency these rhythms.

Язык: Английский