Neural substrates of cold nociception in Drosophila larva
Опубликована: Апрель 29, 2025
Metazoans
detect
and
differentiate
between
innocuous
(non-painful)
and/or
noxious
(harmful)
environmental
cues
using
primary
sensory
neurons,
which
serve
as
the
first
node
in
a
neural
network
that
computes
stimulus
specific
behaviors
to
either
navigate
away
from
injury-
causing
conditions
or
perform
protective
mitigate
extensive
injury.
The
ability
of
an
animal
respond
various
stimuli
depends
upon
molecular
diversity
sensors
underlying
circuitry
responsible
for
relevant
behavioral
action
selection.
Recent
studies
Drosophila
larvae
have
revealed
somatosensory
class
III
multidendritic
(CIII
md)
neurons
function
multimodal
regulating
distinct
responses
mechanical
nociceptive
thermal
stimuli.
advances
circuit
bases
behavior
identified
functionally
validated
larval
involved
(mechanical)
(heat
mechanical)
cues.
However,
central
processing
cold
remained
unexplored.
We
implicate
multisensory
integrators
(Basins),
premotor
(Down-and-Back)
projection
(A09e
TePns)
substrates
required
cold-evoked
calcium
responses.
Neural
silencing
cell
types
downstream
CIII
md
led
significant
reductions
co-activation
plus
additional
facilitated
contraction
(CT)
Further,
we
demonstrate
optogenetic
activation
evokes
increases
these
neurons.
Finally,
characterize
motor
neuron
CT
delineate
muscular
basis
response.
Collectively,
how
process
through
diverse
generating
stimulus-specific
behaviors.
Язык: Английский
Inhibitory circuits coordinate leg movements duringDrosophilagrooming
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Июнь 6, 2024
Limbs
execute
diverse
actions
coordinated
by
the
nervous
system
through
multiple
motor
programs.
The
basic
architecture
of
neurons
that
activate
muscles
articulate
joints
for
antagonistic
flexion
and
extension
movements
is
conserved
from
flies
to
vertebrates.
While
excitatory
premotor
circuits
are
expected
establish
sets
leg
work
together,
our
study
uncovered
a
new
instructive
role
inhibitory
circuits:
their
ability
generate
rhythmic
movements.
Using
electron
microscopy
data
Drosophila
nerve
cord,
we
categorized
~120
GABAergic
13A
13B
hemi-lineages
into
classes
based
on
similarities
in
morphology
connectivity.
By
mapping
synaptic
partners,
pathways
inhibiting
specific
groups
neurons,
disinhibiting
counterparts,
inducing
alternation
between
extension.
We
tested
function
optogenetic
activation
silencing,
using
an
in-depth
ethological
analysis
during
grooming.
combined
anatomy
behavior
findings
construct
computational
model
can
reproduce
major
aspects
observed
behavior,
confirming
sufficiency
these
rhythms.
Язык: Английский