The dinucleotide structure of NAD enables specific reduction on mineral surfaces DOI Open Access
Delfina P. Henriques Pereira,

Xiulan Xie,

Tuğçe Beyazay

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 13, 2024

Abstract Nucleotide-derived cofactors could function as a missing link between the informational and metabolic part at life’s emergence. One well-known example is nicotinamide dinucleotide (NAD), one of evolutionarily most conserved redox found in metabolism. Here, we propose that role these even extend to links geo- biochemistry. We show NAD + can be reduced under close-to nature conditions with nickel-iron-alloys water-rock-interaction settings rich hydrogen (serpentinizing systems) mononucleotide (NMN), precursor molecule NAD, has different properties regarding reduction specificity sensitivity than NAD. The additional adenosine monophosphate (AMP) “tail” dinucleotide, shared trait many organic cofactors, seems play crucial mechanistic preventing overreduction nicotinamide-bearing nucleotide. This also connected used transition metals. While combination nickel iron promotes 1,4-NADH efficiently, case NMN, presence leads accumulation products. Testing reducing abilities both NADH NMNH abiotic showed molecules act equally effective, soluble hydride donors non-enzymatic, proto-metabolic stages

Язык: Английский

An Optimized Deep-Forest Algorithm Using a Modified Differential Evolution Optimization Algorithm: A Case of Host-Pathogen Protein-Protein Interaction Prediction. DOI Creative Commons
Jerry Emmanuel, Itunuoluwa Isewon, Jelili Oyelade

и другие.

Computational and Structural Biotechnology Journal, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0

Peptides En Route from Prebiotic to Biotic Catalysis DOI Creative Commons
Klára Hlouchová

Accounts of Chemical Research, Год журнала: 2024, Номер 57(15), С. 2027 - 2037

Опубликована: Июль 17, 2024

ConspectusIn the quest to understand prebiotic catalysis, different molecular entities, mainly minerals, metal ions, organic cofactors, and ribozymes, have been implied as key players. Of these, inorganic cofactors gained attention for their ability catalyze a wide array of reactions central modern metabolism frequently participate in these within enzymes. Nevertheless, bridging gap between remains fundamental question origins life.In this Account, peptides are investigated potential bridge linking catalysis by minerals/cofactors enzymes that dominate life's chemical reactions. Before ribosomal synthesis emerged, random sequences were plausible on early Earth. This was made possible sources amino acid delivery synthesis, well condensation under variety conditions. Early proteins probably exhibited distinct compositions, enriched small aliphatic acidic residues. An increase abundance acids with larger side chains canonical basic groups most likely dependent emergence more challenging (bio)synthesis. Pressing questions thus arise: how did composition influence peptide properties, what extent could they contribute metabolism?Recent research from our group colleagues shows highly peptides/proteins comprising only presumably "early" fact competent at secondary structure formation even possess adaptive folding characteristics such spontaneous refoldability chaperone independence achieve soluble structures. Moreover, we showed can still bind RNA utilizing ions carboxylate phosphoester functional groups. And finally, ancient shown be capable binding considered prebiotically plausible, supporting properties providing groups, which would nominate them catalytic hubs great relevance.These findings underscore biochemical plausibility an peptide/protein world devoid complex yet collaborating other species. Drawing mechanistic protein-cofactor it is speculated here peptide/protein-cofactor ensemble facilitate similar range reactions, albeit lower rates. hypothesis invites systematic experimental test.Nonetheless, Account does not exclude scenarios prebiotic-to-biotic or prioritize any specific pathways syntheses. The objective examine availability, composition, among various factors involved life.

Язык: Английский

Процитировано

1

Navigating the archaeal frontier: insights and projections from bioinformatic pipelines DOI Creative Commons

Val Karavaeva,

Filipa L. Sousa

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Сен. 23, 2024

Archaea continues to be one of the least investigated domains life, and in recent years, advent metagenomics has led discovery many new lineages at phylum level. For majority, only automatic genomic annotations can provide information regarding their metabolic potential role environment. Here, data from 2,978 archaeal genomes was used perform using bioinformatics tools, alongside synteny analysis. These classifications were done assess how good these different tools relation data. Our study revealed that even with lowered cutoffs, several functional models do not capture recently discovered diversity. Moreover, our investigation a significant portion genomes, approximately 42%, remain uncharacterized. In comparison, within 3,235 bacterial diverse range unclassified proteins is obtained, well-studied organisms like Escherichia coli having substantially lower proportion uncharacterized regions, ranging <5 25%, less studied being comparable archaea 35-40% regions. Leveraging this analysis, we able identify protein markers, thereby providing insights into metabolism dataset. findings underscore substantial gap between classification comprehensive mapping metabolism. Despite advances computational approaches, remains unexplored, highlighting need for extensive experimental validation domain, as well more refined annotation methods. This contributes better understanding underscores importance further research elucidating genomes.

Язык: Английский

Процитировано

0

The dinucleotide structure of NAD enables specific reduction on mineral surfaces DOI Open Access
Delfina P. Henriques Pereira,

Xiulan Xie,

Tuğçe Beyazay

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 13, 2024

Abstract Nucleotide-derived cofactors could function as a missing link between the informational and metabolic part at life’s emergence. One well-known example is nicotinamide dinucleotide (NAD), one of evolutionarily most conserved redox found in metabolism. Here, we propose that role these even extend to links geo- biochemistry. We show NAD + can be reduced under close-to nature conditions with nickel-iron-alloys water-rock-interaction settings rich hydrogen (serpentinizing systems) mononucleotide (NMN), precursor molecule NAD, has different properties regarding reduction specificity sensitivity than NAD. The additional adenosine monophosphate (AMP) “tail” dinucleotide, shared trait many organic cofactors, seems play crucial mechanistic preventing overreduction nicotinamide-bearing nucleotide. This also connected used transition metals. While combination nickel iron promotes 1,4-NADH efficiently, case NMN, presence leads accumulation products. Testing reducing abilities both NADH NMNH abiotic showed molecules act equally effective, soluble hydride donors non-enzymatic, proto-metabolic stages

Язык: Английский

Процитировано

0