bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Июль 1, 2024
The maternal-to-zygotic transition (MZT) is a conserved developmental process where the maternally-derived protein and mRNA cache replaced with newly made zygotic gene products. We have previously shown that in
Язык: Английский
EMBO Reports, Год журнала: 2025, Номер unknown
Опубликована: Фев. 20, 2025
Abstract The maternal-to-zygotic transition (MZT) is a conserved developmental process where the maternally-derived protein and mRNA cache replaced with newly made zygotic gene products. We have previously shown that in Drosophila deposited RNA-binding proteins ME31B, Cup, Trailer Hitch are ubiquitylated by CTLH E3 ligase cleared. However, organization regulation of complex remain poorly understood flies because lacks an identifiable substrate adaptor, mechanisms restricting degradation ME31B its cofactors to MZT unknown. Here, we show multi-pronged, including transcriptional control OVO autoinhibition ligase. One major regulatory target subunit Muskelin, which demonstrate adaptor for complex. Finally, find Muskelin has few targets beyond three known proteins, showing exquisite specificity. Thus, multiple levels integrated restrict activity embryonic early embryogenesis, during time it regulates important proteins.
Язык: Английский
Процитировано
0
Genes & Development, Год журнала: 2024, Номер unknown
Опубликована: Дек. 20, 2024
The gene-regulatory mechanisms controlling the expression of germline PIWI-interacting RNA (piRNA) pathway components within gonads metazoan species remain largely unexplored. In contrast to male piRNA pathway, which in mice is known be activated by testis-specific transcription factor A-MYB, nature ovary-specific network driving female remains a mystery. Here, using Drosophila as model, we combined multiple genomics approaches reveal Ovo regulator ovarian germ cells. Ectopic somatic cells activates components, including ping-pong factors Aubergine, Argonaute-3, and Vasa, leading assembly perinuclear cellular structures resembling nuage bodies We found that cells, ovo repressed l(3)mbt , thus preventing genes soma. Cross-species ChIP-seq motif analyses demonstrate binding genomic CCGTTA motifs promoters genes, suggesting regulation ovaries analogous A-MYB testes. Our results also show consistent engagement family at clusters across species, reflecting deep evolutionary conservation this regulatory paradigm from insects humans.
Язык: Английский
Процитировано
3
Open Biology, Год журнала: 2025, Номер 15(4)
Опубликована: Апрель 1, 2025
Global climate change is characterized by increased extreme temperatures affecting insects at all trophic levels. Zinc finger proteins (ZFPs) are key regulators of gene expression and cell differentiation in eukaryotes, essential for stress resistance both animals plants. Using CRISPR/Cas9 deletion, this study predicted examined the structure ZFP320 diamondback moth ( Plutella xylostella ) investigated its function temperature response through a comprehensive age-stage, two-sex life table analysis. We found encodes 387 amino acid protein (43 kDa) with no transmembrane domains, featuring ZnF-C2H2 domain. Quantitative fluorescence analysis showed that under high temperatures. knockout altered antioxidant expression, resulting higher levels superoxide dismutase catalase mutant strains compared wild-type strain. Life revealed had shorter fecundity oviposition periods normal Additionally, exhibited lower parameters r , λ R 0 ), as well reduced survival rates critical thermal maxima. Notably, PxZFP320 plays crucial role adaptation, paving way future investigations on significance ZFPs P. ’s tolerance.
Язык: Английский
Процитировано
0
Developmental Biology, Год журнала: 2025, Номер 523, С. 111 - 114
Опубликована: Апрель 23, 2025
Язык: Английский
Процитировано
0
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Июль 1, 2024
The maternal-to-zygotic transition (MZT) is a conserved developmental process where the maternally-derived protein and mRNA cache replaced with newly made zygotic gene products. We have previously shown that in
Язык: Английский
Процитировано
0