Frontiers in Bioengineering and Biotechnology,
Год журнала:
2024,
Номер
12
Опубликована: Ноя. 22, 2024
Introduction
Heterotopic
ossification
(HO)
occurs
following
orthopedic
trauma,
spinal
cord
injuries,
brain
trauma
and
limb
amputations.
Once
symptomatic,
HO
causes
pain,
limited
mobility
decreased
quality
of
life.
Current
treatments
are
have
significant
complications
with
high
recurrence
rates,
underscoring
the
need
for
improved
therapeutic
interventions.
Osteoclasts
(OCs)
physiological
bone
resorptive
cells
that
secrete
enzymes
protons
to
degrade
bone.
Methods
In
this
study,
we
describe
use
genetically
engineered
OCs
as
a
novel
cell
therapy
approach
treat
HO.
Inducible,
myeloid
precursors
(iRANK
cells)
treated
chemical
inducer
dimerization
(CID)
differentiated
into
TRAP
+
multinucleated
resorbed
mineralized
tissues
in
vitro
.
Results
vivo
,
BMP-2-induced
murine
lesions
were
significantly
regressed
treatment
using
iRANK
concomitant
systemic
administration
CID.
Moreover,
many
MMP9
GFP
indicating
they
from
delivered
cells.
Discussion
summary,
these
data
con
rm
ability
differentiate
resorb
paving
way
OC
delivery
promising
treatment.
Journal of Functional Biomaterials,
Год журнала:
2024,
Номер
15(8), С. 211 - 211
Опубликована: Июль 29, 2024
Polydopamine,
a
mussel-inspired
self-adherent
polymer
of
dopamine,
has
impressive
adhesive
properties
and
thus
is
one
the
most
versatile
approaches
to
functionalize
tissue
engineering
scaffolds.
To
date,
many
types
polydopamine-functionalized
scaffolds
have
been
manufactured
extensively
applied
in
bone
at
preclinical
stage.
However,
how
polydopamine
biodegraded
metabolized
during
healing
process
side
effects
its
metabolite
remain
largely
unknown.
These
issues
are
often
neglected
modern
manufacture
materials
restrict
them
from
stepping
forward
clinical
applications.
In
this
study,
using
our
bioinspired
polydopamine-laced
hydroxyapatite
collagen
calcium
silicate
material
as
representative
scaffolds,
we
discovered
that
can
be
dopamine
specifically
by
osteoclasts,
which
termed
“osteoclast-driven
polydopamine-to-dopamine
release”.
The
released
showed
an
osteoinductive
effect
vitro
promoted
regeneration
calvarial
critical-sized
defects.
concept
release”
considerable
application
potential.
It
could
easily
adopted
other
existing
scaffolds:
just
recruiting
osteoclasts.
Once
adopted,
will
obtain
dopamine-releasing
function,
enables
their
modulation
osteoblast
activity
hence
elevates
effect.
Thus,
serves
upgrade
patch,
useful
for
materials.
Progress in Orthodontics,
Год журнала:
2025,
Номер
26(1)
Опубликована: Янв. 2, 2025
Abstract
Background
Yes-associated
protein
(YAP)
is
a
crucial
mechanosensor
involved
in
mechanotransduction,
but
its
role
regulating
mechanical
force-induced
bone
remodeling
during
orthodontic
tooth
movement
(OTM)
unclear.
This
study
aims
to
elucidate
the
relationship
between
mechanotransduction
and
alveolar
OTM.
Results
Our
confirms
an
asynchronous
(temporal
spatial
sequence)
pattern
of
under
force
Both
compression
tension
activate
osteoclasts
recruiting
bone,
whereas
no
significant
presence
osteoblasts
at
early
stages
remodeling.
Specifically,
applying
different
magnitudes
(10,
25,
50,
100
g)
rats’
1st
molars
affected
OTM
distance.
Force-induced
was
characterized
by
osteoclastogenesis
YAP
activation
compressive/tensile
sites
on
day
1
Notably,
25
g
triggered
peak
expression
osteoclastic
activity
on.
Time-course
analysis
revealed
two
peaks
day1
14,
contrasting
with
one
type
I
collagen
day14.
In
addition,
RNA-sequencing
highlighted
increased
nuclear
factor
kappa
B
(NF-κB)
signaling,
mineral
absorption,
osteoclast
differentiation
day-1
3.
Moreover,
gene
showed
similar
trends
for
NF-κB
p65,
YAP1,
TEA
domain
(TEAD1)
this
time.
Furthermore,
experiments
cultures
indicated
via
large
tumor
suppressor
(LATS)
TEAD
stimuli
(compression/tension),
promoting
p65
receptor
activator
(RANK).
Inhibiting
verteporfin
delayed
impairing
activities
vivo
ex-vivo.
Conclusions
We
propose
that
mediates
through
p65-induced
remodeling,
offering
evidence
orthodontists
as
reference.
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Янв. 2, 2025
Abstract
Lysophosphatidylethanolamine
(LPE)
is
a
bioactive
lipid
mediator
involved
in
diverse
cellular
functions.
In
this
study,
we
investigated
the
effects
of
three
LPE
species,
1-palmitoyl
(16:0
LPE),
1-stearoyl
(18:0
and
1-oleoyl
(18:1
LPE)
on
pre-osteoblast
MC3T3-E1
cells.
All
species
stimulated
cell
proliferation
activated
mitogen-activated
protein
kinase
(MAPK)/extracellular
signal-regulated
(ERK)
1/2.
MAPK/ERK1/2
activation
by
16:0
18:1
was
inhibited
Gq/11
inhibitor
YM-254890,
while
18:0
blocked
Gi/o
pertussis
toxin.
Intracellular
Ca
2+
transients
were
triggered
but
not
LPE,
with
YM-254890
suppressing
these
responses.
These
results
suggest
that
act
via
Gq/11-coupled
G
coupled
receptors
(GPCRs),
acts
Gi/o-coupled
GPCRs.
Furthermore,
receptor
desensitization
experiments
suggested
each
through
distinct
Interestingly,
suppressed
osteogenic
differentiation,
reducing
mineralization,
alkaline
phosphatase
activity,
gene
expression,
whereas
had
no
such
effects.
physiological
significance
LPEs
bone
formation
indicate
different
their
play
distinctive
roles
process.
Review
Lifting
the
Veil
on
Myeloma
Bone
Disease
Rui
Chen
1,
Liu
and
Huan
1,2,*
1
Cancer
Research
Center,
School
of
Medicine,
Xiamen
University,
361102,
China
2
Department
Hematology,
The
First
Affiliated
Hospital
University
Institute
*
Correspondence:
[email protected]
Received:
13
November
2024;
Revised:
29
November;
Accepted:
January
2025;
Published:
23
2025
Abstract:
Multiple
myeloma
(MM),
a
hematological
malignancy
originating
from
malignant
plasma
cells
in
bone
marrow,
predominantly
affects
elderly,
its
incidence
is
rise.
It
currently
second
most
common
malignancy.
Osteolytic
disease,
severe
complication
detected
nearly
80%
patients,
entire
skeletal
system,
particularly
skull,
spine,
pelvis,
long
bones
limbs.
This
condition
causes
pathological
fractures,
pain,
spinal
cord
compression,
hypercalcemia.
management
damage
patients
presents
numerous
challenges,
with
current
clinical
treatments
primarily
relying
bisphosphonates
anti-RANKL
monoclonal
antibodies
(Denosumab).
review
summarizes
recent
advancements
research
damage,
focusing
complex
interactions
between
various
other
cell
types
that
affect
skeleton.
also
discusses
challenges
encountered
research,
highlighting
potential
future
directions
proposing
therapeutic
strategies.
