Frontiers in Bioengineering and Biotechnology,
Год журнала:
2024,
Номер
12
Опубликована: Ноя. 22, 2024
Introduction
Heterotopic
ossification
(HO)
occurs
following
orthopedic
trauma,
spinal
cord
injuries,
brain
trauma
and
limb
amputations.
Once
symptomatic,
HO
causes
pain,
limited
mobility
decreased
quality
of
life.
Current
treatments
are
have
significant
complications
with
high
recurrence
rates,
underscoring
the
need
for
improved
therapeutic
interventions.
Osteoclasts
(OCs)
physiological
bone
resorptive
cells
that
secrete
enzymes
protons
to
degrade
bone.
Methods
In
this
study,
we
describe
use
genetically
engineered
OCs
as
a
novel
cell
therapy
approach
treat
HO.
Inducible,
myeloid
precursors
(iRANK
cells)
treated
chemical
inducer
dimerization
(CID)
differentiated
into
TRAP
+
multinucleated
resorbed
mineralized
tissues
in
vitro
.
Results
vivo
,
BMP-2-induced
murine
lesions
were
significantly
regressed
treatment
using
iRANK
concomitant
systemic
administration
CID.
Moreover,
many
MMP9
GFP
indicating
they
from
delivered
cells.
Discussion
summary,
these
data
con
rm
ability
differentiate
resorb
paving
way
OC
delivery
promising
treatment.
Effectively
addressing
inflammation
in
periodontitis
is
challenging
as
conventional
injectable
hydrogels
typically
require
the
addition
of
drugs
to
provide
sufficient
anti-inflammatory
effects.
To
overcome
this
limitation,
we
developed
a
multifunctional
hydrogel
with
inherent
properties
that
antagonize
Toll-like
receptor
4
and
myeloid
differentiation
factor
2
complex
(TLR4-MD2).
This
allows
for
direct
inhibition
inflammatory
pathways
without
need
additional
drugs.
We
identified
xylitol,
caffeic
acid,
citric
acid
natural
materials
effectively
meet
biological
needs
antibacterial
effects
well
support
bone
regeneration.
With
mind,
caffeic-acid-modified
poly(xylitol
succinate)
(PXS)-based
iCPC@MgO
composite
tested
its
potential
application
periodontal
The
demonstrated
rapid
wet
tissue
adhesion
injectability,
which
are
ascribed
incorporating
catechol
groups
derived
from
acid.
Intriguingly,
PXS
polymer
used
synthesizing
was
found
possess
act
an
antagonist
TLR4-MD2
complex.
also
exhibited
outstanding
efficiency
against
Porphyromonas
gingivalis
Aggregatibacter
actinomycetemcomitans
by
stimulating
antibiotic
synthesis
within
bacteria
disrupting
bacterial
cell
walls.
In
mouse
model,
therapeutic
reducing
factors,
inhibiting
dominant
periodontitis-associated
bacteria,
maintaining
subgingival
microbiota
balance
regenerative
These
properties,
combined
their
ecofriendly
nature,
firmly
established
highly
promising
option
use
therapy
healing,
repair,
regeneration
various
other
conditions.
Bone Reports,
Год журнала:
2025,
Номер
25, С. 101836 - 101836
Опубликована: Март 12, 2025
Allografts
play
an
important
role
in
treatment
of
complex
bone
fractures
and
deformities.
The
purpose
this
study
was
to
test
the
hypothesis
that
alcohol
consumption
impairs
graft
incorporation
healing
by
two
mechanisms:
(1)
lowering
osteoinductive
capacity
(2)
suppressing
formation.
We
performed
experiments
using
a
demineralized
allogeneic
matrix
(DBM)
model
which
DBM
harvested
from
donor
rats
fed
control
or
ethanol
diet
implanted
subcutaneously
into
recipient
diet.
also
evaluated
efficacy
intermittent
parathyroid
hormone
(PTH)
on
(DBM
diet)
critical
size
defect
model.
Bone
formed
during
osteoinduction
measured
micro-computed
tomography.
Experiment
1:
volume
lower
ethanol-consuming
donors
6
weeks
following
implantation
recipients
diet,
indicating
exposure
lowered
capacity.
2:
3
compared
recipients.
3:
Ethanol
resulted
tendency
for
(p
=
0.085)
whereas
PTH
higher
Our
results
suggest
chronic
heavy
allograft
may
impair
negative
outcome
be
worsened
intake
healing.
Additionally,
has
potential
increase
both
abstinent
alcohol-consuming
donors.
International Journal of Oral Science,
Год журнала:
2025,
Номер
17(1)
Опубликована: Апрель 1, 2025
Abstract
Corticotomy
is
a
clinical
procedure
to
accelerate
orthodontic
tooth
movement
characterized
by
the
regional
acceleratory
phenomenon
(RAP).
Despite
its
therapeutic
effects,
surgical
risk
and
unclear
mechanism
hamper
application.
Numerous
evidences
support
macrophages
as
key
immune
cells
during
bone
remodeling.
Our
study
discovered
that
monocyte-derived
primarily
exhibited
pro-inflammatory
phenotype
dominated
remodeling
in
corticotomy
CX3CR1
CreERT2
;
R26
GFP
lineage
tracing
system.
Fluorescence
staining,
flow
cytometry
analysis,
western
blot
determined
significantly
enhanced
expression
of
binding
immunoglobulin
protein
(BiP)
emphasized
activation
sensor
activating
transcription
factor
6
(ATF6)
macrophages.
Then,
we
verified
macrophage
specific
ATF6
deletion
(ATF6
f/f
mice)
decreased
proportion
therefore
blocked
acceleration
effect
corticotomy.
In
contrast,
overexpression
exaggerated
movement.
vitro
experiments
also
proved
higher
was
positively
correlated
with
ATF6.
At
level,
RNA-seq
CUT&Tag
analysis
demonstrated
modulated
macrophage-orchestrated
inflammation
through
interacting
Tnfα
promotor
augmenting
transcription.
Additionally,
molecular
docking
simulation
dual-luciferase
reporter
system
indicated
possible
sites
outside
traditional
endoplasmic
reticulum-stress
response
element
(ERSE).
Taken
together,
may
aggravate
promoting
macrophages,
suggesting
represent
promising
target
for
non-invasive
accelerated
orthodontics.
Stem Cell Research & Therapy,
Год журнала:
2025,
Номер
16(1)
Опубликована: Апрель 12, 2025
Mesenchymal
stem
cells
(MSCs)
play
a
crucial
role
in
bone
formation
and
remodeling.
Intrinsic
genetic
factors
extrinsic
environmental
cues
regulate
their
differentiation
into
osteoblasts.
Within
the
microenvironment,
complex
network
of
biochemical
biomechanical
signals
orchestrates
homeostasis
regeneration.
In
addition,
crosstalk
among
MSCs,
immune
cells,
neighboring
cells-mediated
by
extracellular
vesicles
non-coding
RNAs
(such
as
circular
micro
RNAs)
-profoundly
influences
osteogenic
Recent
studies
have
explored
specific
signaling
pathways
that
contribute
to
effective
regeneration,
highlighting
potential
manipulating
microenvironment
enhance
MSC
functionality.
The
integration
advanced
biomaterials,
gene
editing
techniques,
controlled
delivery
systems
is
paving
way
for
more
targeted
efficient
regenerative
therapies.
Furthermore,
artificial
intelligence
could
improve
tissue
engineering,
optimize
biomaterial
design,
enable
personalized
treatment
strategies.
This
review
explores
latest
advancements
emphasizing
intricate
interplay
molecules.
By
providing
comprehensive
overview
these
mechanisms
clinical
implications,
we
aim
shed
light
on
future
research
directions
this
rapidly
evolving
field.
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2025,
Номер
13
Опубликована: Апрель 14, 2025
The
development
of
new
artificial
biomaterials
for
bone
defect
repair
is
an
ongoing
area
clinical
research.
Metal
ions
such
as
zinc,
copper,
magnesium,
calcium,
strontium,
silver,
and
cerium
play
various
roles
in
tissue
regeneration
the
human
body
possess
a
range
biochemical
functions.
Studies
have
demonstrated
that
appropriate
concentrations
these
metal
can
promote
osteogenesis
angiogenesis,
inhibit
osteoclast
activity,
deter
bacterial
infections.
Researchers
incorporated
into
using
methods
to
create
materials
with
enhanced
osteogenic
antibacterial
capabilities.
In
addition
properties
all
aforementioned
ions,
Zn,
Sr,
Ce
indirectly
by
inhibiting
activity.
Cu,
Mg,
Sr
significantly
enhance
while
Ag,
reduce
likelihood
infection
inflammation
caused
implanted
materials.
This
paper
reviews
mechanisms
through
which
growth
improve
activity
biomaterials.
It
also
summarizes
common
loading
on
surface
different
metals
highlights
potential
applications
Biomaterials,
Год журнала:
2025,
Номер
322, С. 123377 - 123377
Опубликована: Май 1, 2025
To
date,
cell-based
approaches
to
stimulate
bone
formation
have
primarily
focused
on
mesenchymal
stromal
cells
(MSCs)
for
their
supposed
osteogenic
potential,
but
despite
some
pre-clinical
successes,
clinical
outcomes
remained
unsatisfactory.
Emerging
data
suggest
that
osteoclasts
play
crucial
roles
in
stimulating
beyond
catabolic
function
resorption.
Interestingly,
osteoclastic
activity
precedes
osteoblastic
the
physiological
remodeling
cycle.
explore
role
of
further,
we
prepared
osteoclast-based
constructs
and
implanted
them
(i)
ectopically
evaluate
potential
induce
formation,
(ii)
orthotopically
effects
regeneration.
Remarkably,
containing
primary
mouse
showed
consistent
robust
de
novo
which
presented
comparable
efficacy
BMP-2
treatment.
Additionally,
observed
marrow
upon
ectopic
implantation
(incidence
73
%)
loaded
controls
91
%).
Importantly,
macrophages
(MФs)
or
scaffold
only
(negative
control)
neither
nor
formation.
Further,
a
cranial
defect
model
confirmed
stimulatory
regeneration
capabilities
Osteoclast-based
constructs,
evidenced
by
2.5-fold
increased
compared
only.
These
findings
demonstrate
osteoinduction
osteogenesis
capacity
osteoclasts,
reshaping
our
understanding
opening
new
avenues
design
development
repair.
