Exploring the molecular composition of the multipass translocon in its native membrane environment DOI Creative Commons
Max Gemmer, Marten L. Chaillet, Friedrich Förster

и другие.

Life Science Alliance, Год журнала: 2024, Номер 7(8), С. e202302496 - e202302496

Опубликована: Июнь 12, 2024

Multispanning membrane proteins are inserted into the endoplasmic reticulum by ribosome-bound multipass translocon (MPT) machinery. Based on cryo-electron tomography and extensive subtomogram analysis, we reveal composition arrangement of MPT components in their native environment. The intramembrane chaperone complex PAT translocon-associated protein (TRAP) associate substoichiometrically with a translation-dependent manner. Although is preferentially part MPTs bound to translating ribosomes, abundance TRAP highest associated non-translating ribosomes. average TRAP-containing reveals intermolecular contacts between luminal domains an unknown subunit back-of-Sec61 complex. AlphaFold modeling suggests this nodal modulator, bridging nicalin TRAPα. Collectively, our results visualize variability factors environment dependent translational activity ribosome.

Язык: Английский

Exploring the molecular composition of the multipass translocon in its native membrane environment DOI Creative Commons
Max Gemmer, Marten L. Chaillet, Friedrich Förster

и другие.

Life Science Alliance, Год журнала: 2024, Номер 7(8), С. e202302496 - e202302496

Опубликована: Июнь 12, 2024

Multispanning membrane proteins are inserted into the endoplasmic reticulum by ribosome-bound multipass translocon (MPT) machinery. Based on cryo-electron tomography and extensive subtomogram analysis, we reveal composition arrangement of MPT components in their native environment. The intramembrane chaperone complex PAT translocon-associated protein (TRAP) associate substoichiometrically with a translation-dependent manner. Although is preferentially part MPTs bound to translating ribosomes, abundance TRAP highest associated non-translating ribosomes. average TRAP-containing reveals intermolecular contacts between luminal domains an unknown subunit back-of-Sec61 complex. AlphaFold modeling suggests this nodal modulator, bridging nicalin TRAPα. Collectively, our results visualize variability factors environment dependent translational activity ribosome.

Язык: Английский

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