Biochemical Society Transactions, Год журнала: 2025, Номер 53(02), С. 431 - 445

Опубликована: Апрель 1, 2025

The endosomal system is essential for the intra- and intercellular communication in cells multicellular organisms. It involved secretion of signaling factors serves as a venue receptors from plasma membrane, which are endocytosed after ligand binding. Many internalized receptor–ligand complexes numerous other proteins arrive at Rab5-positive early endosome, where they will be sorted. Cargoes marked with ubiquitin bound by sorting complex required transport (ESCRT)-0 ESCRT-I to initiate their degradation. remaining cargoes recycled back membrane or trans-Golgi network. To degrade ubiquitinated cargoes, endosome has mature into late structure, multivesicular body (MVB). This procedure requires Rab5-to-Rab7 conversion, mediated RABEX5-MON1/CCZ1 RabGEF cascade. Moreover, destined degradation have packaged intraluminal vesicles (ILVs) through ESCRT-III Vps4. matured MVB finally fuses lysosome cargo. Although ESCRT-mediated ILV formation Rab conversion well-characterized processes during maturation, it remained until recently unclear whether these connected. Lately, several studies were published illuminating relationship ESCRT functions conversion. Here, we review current knowledge on role machinery cargo RABEX5 regulation MON1/CCZ1-mediated maturation. propose model regulatory

Язык: Английский