Synaptic connectome of a neurosecretory network in the Drosophila brain

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 29, 2024

Abstract Hormones mediate inter-organ signaling which is crucial in orchestrating diverse behaviors and physiological processes including sleep activity, feeding, growth, metabolism reproduction. The pars intercerebralis lateralis insects represent major hubs contain neurosecretory cells (NSC) that produce various hormones. To obtain insight into how hormonal regulated, we have characterized the synaptic connectome of NSC adult Drosophila brain. Identification neurons providing inputs to multiple subtypes implicates diuretic hormone 44-expressing as a coordinator physiology behavior. Surprisingly, despite most having dendrites subesophageal zone (primary taste processing center), gustatory are largely indirect. We also deciphered pathways via olfactory relayed NSC. Further, our analyses revealed substantial from descending NSC, suggesting regulate both endocrine motor output synchronize changes with appropriate behaviors. In contrast inputs, sparse mostly mediated by corazonin Therefore, additionally determine putative paracrine interconnectivity between peripheral tissues analyzing single-cell transcriptomic datasets. Our comprehensive characterization network provides platform understand complex networks they orchestrate animal physiology.

Язык: Английский

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What Drosophila can tell us about state-dependent peptidergic signaling in insects

Insect Biochemistry and Molecular Biology, Год журнала: 2025, Номер unknown, С. 104275 - 104275

Опубликована: Фев. 1, 2025

Язык: Английский

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Proteome-wide neuropeptide identification using NeuroPeptide-HMMer (NP-HMMer)

General and Comparative Endocrinology, Год журнала: 2024, Номер 357, С. 114597 - 114597

Опубликована: Июль 29, 2024

Neuropeptides are essential neuronal signaling molecules that orchestrate animal behavior and physiology via actions within the nervous system on peripheral tissues. Due to small size of biologically active mature peptides, their identification a proteome-wide scale poses significant challenge using existing bioinformatics tools like BLAST. To address this, we have developed NeuroPeptide-HMMer (NP-HMMer), hidden Markov model (HMM)-based tool facilitate neuropeptide discovery, especially in underexplored invertebrates. NP-HMMer utilizes manually curated HMMs for 46 families, enabling rapid accurate neuropeptides. Validation Drosophila melanogaster, Daphnia pulex, Tribolium castaneum Tenebrio molitor demonstrated its effectiveness identifying known neuropeptides across diverse arthropods. Additionally, showcase utility by discovering novel Priapulida Rotifera, 22 19 new respectively. This represents advancement research, offering robust method annotating proteomes providing insights into evolutionary conservation pathways.

Язык: Английский

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Ion transport peptide and ion transport peptide-like regulate ecdysis behavior and water transport during ecdysis in Gryllus bimaculatus

Insect Biochemistry and Molecular Biology, Год журнала: 2024, Номер 173, С. 104178 - 104178

Опубликована: Авг. 24, 2024

Ion transport peptide (ITP) and ITP-like (ITPLs) are pleiotropic bioactive peptides in insects. Although the contribution of these to ecdysis has been studied, precise regulatory mechanisms remain poorly understood. Here, we characterized functions itp itpl variants two-spotted cricket, Gryllus bimaculatus. Reverse transcription-quantitative PCR whole-mount situ hybridization revealed that was expressed brain terminal abdominal ganglion, whereas were all ganglia central nervous system. Simultaneous knockdown itpls disrupted behavior water from gut into hemolymph during molting. Nevertheless, without influencing expression did not significantly affect but caused a reduction mass. is considered necessary for swelling required split old cuticle layers molting, rescue experiment by injection or cricket Ringer's solution crickets recover normal phenotype. Therefore, propose ITP/ITPL control probably regulating crickets.

Язык: Английский

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Insulin/insulin-like growth factor signaling pathway promotes higher fat storage inDrosophilafemales

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 18, 2024

Summary In Drosophila , adult females store more fat than males. While the mechanisms that restrict body in males are becoming clearer, less is known about how achieve higher storage. Here, we perform a detailed investigation of promote storage females. We show greater intake dietary sugar supports due to female-biased remodeling lipidome. Dietary stimulates female-specific increase insulin-like peptide 3 (Dilp3), which acts together with peripheral insulin sensitivity augment insulin/insulin-like growth factor signaling pathway (IIS) activity Given adult-specific IIS inhibition reduced sex difference decrease fat, our data reveal as key determinant female

Язык: Английский

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Insulin/Insulin-Like Growth Factor Signaling Pathway Promotes Higher Fat Storage in Drosophila Females

Опубликована: Янв. 1, 2024

Язык: Английский

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