Genome concentration limits cell growth and modulates proteome composition in Escherichia coli
eLife,
Год журнала:
2024,
Номер
13
Опубликована: Май 10, 2024
Defining
the
cellular
factors
that
drive
growth
rate
and
proteome
composition
is
essential
for
understanding
manipulating
systems.
In
bacteria,
ribosome
concentration
known
to
be
a
constraining
factor
of
cell
rate,
while
gene
usually
assumed
not
limiting.
Here,
using
single-molecule
tracking,
quantitative
single-cell
microscopy,
modeling,
we
show
genome
dilution
in
Escherichia
coli
cells
arrested
DNA
replication
limits
total
RNA
polymerase
activity
within
physiological
sizes
across
tested
nutrient
conditions.
This
rapid-onset
limitation
on
bulk
transcription
results
sub-linear
scaling
active
ribosomes
with
size
sub-exponential
growth.
Such
downstream
effects
translation
are
near-immediately
detectable
nutrient-rich
medium,
but
delayed
nutrient-poor
conditions,
presumably
due
buffering
activities.
sequencing
tandem-mass-tag
mass
spectrometry
experiments
further
reveal
remodels
relative
abundance
mRNAs
proteins
at
global
level.
Altogether,
our
findings
indicate
chromosome
limiting
modulator
transcriptome
E.
.
Experiments
Caulobacter
crescentus
comparison
eukaryotic
studies
identify
broadly
conserved
concentration-dependent
principles
expression.
Язык: Английский
Borrelia burgdorferi loses essential genetic elements and cell proliferative potential during stationary phase in culture but not in the tick vector
Journal of Bacteriology,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 14, 2025
ABSTRACT
The
Lyme
disease
agent
Borrelia
burgdorferi
is
a
polyploid
bacterium
with
segmented
genome
in
which
both
the
chromosome
and
over
20
distinct
plasmids
are
present
multiple
copies
per
cell.
This
pathogen
can
survive
for
at
least
9
months
its
tick
vector
an
apparent
dormant
state
between
blood
meals,
without
losing
cell
proliferative
capability
when
re-exposed
to
nutrients.
Cultivated
B.
cells
grown
stationary
phase
or
resuspended
nutrient-limited
media
often
used
study
effects
of
nutrient
deprivation.
However,
thorough
assessment
spirochete’s
ability
recover
from
depletion
has
been
lacking.
Our
shows
that
starved
cultures
rapidly
lose
ability.
Loss
genetic
elements
essential
proliferation
contributes
observed
defect
phase.
gradual
decline
not
perfectly
synchronized
chromosomes
plasmids,
generating
harbor
one
more
but
lack
all
non-essential
plasmids.
phenomenon
likely
well-documented
issue
plasmid
loss
during
vitro
cultivation
.
In
contrast,
ticks
14
showed
no
evidence
reduced
loss.
Beyond
their
practical
implications
studying
,
these
findings
suggest
midgut
offers
unique
environment
supports
maintenance
’s
potential
periods
fasting.
IMPORTANCE
causes
disease,
prevalent
tick-borne
illness.
must
long
(months
year)
dormancy
meals.
Resilience
starvation
common
trait
among
bacteria.
this
reveals
that,
laboratory
cultures,
poorly
endures
loses
viability.
linked
required
proliferation.
These
results
persistence
nature
shaped
by
environmental
conditions
than
innate
endure
Язык: Английский
Extrusion-modulated DnaA activity oscillations coordinate DNA replication with biomass growth
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2025,
Номер
unknown
Опубликована: Май 13, 2025
Abstract
Robust
control
of
DNA
replication
is
fundamental
to
bacterial
proliferation.
In
Escherichia
coli
,
initiation
thought
be
regulated
by
oscillations
in
DnaA
activity,
driven
DnaA-chromosome
interactions
that
differ
among
leading
models.
However,
direct
evidence
linking
these
has
been
lacking,
and
existing
models
fail
explain
the
observed
decoupling
from
dnaA
expression.
Here,
we
establish
a
link
between
activity
demonstrating
robust
which
peak
precisely
at
across
diverse
growth
conditions
genetic
perturbations.
Notably,
persist
even
when
transcription
remains
constant,
suggesting
regulatory
mechanism
modulates
independently
its
Additionally,
propose
an
extrusion
model
DNA-binding
proteins
sense
biomass-DNA
imbalance
extrude
chromosome
trigger
replication,
overcoming
limitations
Consistent
with
this
model,
perturbation
nucleoid-associated
protein
H-NS
timing,
supporting
mechanistic
validity.
Язык: Английский
Borrelia burgdorferi loses essential genetic elements and cell proliferative potential during stationary phase in culture but not in the tick vector.
Опубликована: Окт. 28, 2024
The
Lyme
disease
agent
Язык: Английский
Dynamic cytoplasmic fluidity during morphogenesis in a human fungal pathogen
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 17, 2024
Abstract
The
molecular
crowding
of
the
cytoplasm
impacts
a
range
cellular
processes.
Using
fluorescent
microrheological
probe
(GEMs),
we
observed
striking
decrease
in
during
yeast
to
filamentous
growth
transition
human
fungal
pathogen
Candida
albicans
.
This
is
due
ribosome
concentration
that
results
part
from
an
inhibition
biogenesis,
combined
with
increase
cytoplasmic
volume;
leading
dilution
major
crowder.
Moreover,
our
suggest
biogenesis
trigger
for
C.
morphogenesis.
Язык: Английский