A spatial single-cell atlas of the claustro-insular region uncovers key regulators of neuronal identity and excitability DOI Creative Commons
Leon Fodoulian, Madlaina Boillat,

Marie Moulinier

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 5, 2024

Abstract The claustro-insular region is an evolutionarily conserved and extensively interconnected brain area, critical for functions such as attention, cognitive flexibility, interoception, affective processing. Despite its importance, cellular composition organization remain poorly characterized, hindering a comprehensive understanding of the mechanisms underlying diverse functions. By combining single-cell RNA sequencing spatial transcriptomics, we created high-resolution atlas this in mice, uncovering distinct neuronal subtypes unexpected complexity. Leveraging atlas, investigated role NR4A2, neuropsychiatric risk factor expressed several subtypes. In Nr4a2 haploinsufficiency model, found that only claustrum neurons exhibited shifts molecular identity. This identity shift, which involved activation transcription cascade, was associated with alterations firing activity. Our findings provide new insights into architecture highlights master regulator component’s identities.

Язык: Английский

A spatial single-cell atlas of the claustro-insular region uncovers key regulators of neuronal identity and excitability DOI Creative Commons
Leon Fodoulian, Madlaina Boillat,

Marie Moulinier

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 5, 2024

Abstract The claustro-insular region is an evolutionarily conserved and extensively interconnected brain area, critical for functions such as attention, cognitive flexibility, interoception, affective processing. Despite its importance, cellular composition organization remain poorly characterized, hindering a comprehensive understanding of the mechanisms underlying diverse functions. By combining single-cell RNA sequencing spatial transcriptomics, we created high-resolution atlas this in mice, uncovering distinct neuronal subtypes unexpected complexity. Leveraging atlas, investigated role NR4A2, neuropsychiatric risk factor expressed several subtypes. In Nr4a2 haploinsufficiency model, found that only claustrum neurons exhibited shifts molecular identity. This identity shift, which involved activation transcription cascade, was associated with alterations firing activity. Our findings provide new insights into architecture highlights master regulator component’s identities.

Язык: Английский

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