Resuspension and Dissemination of MS2 Virus from Flooring After Human Activities in Built Environment: Impact of Dust Particles DOI Creative Commons

Stephanie A. Boone,

M. Khalid Ijaz, Julie McKinney

и другие.

Microorganisms, Год журнала: 2024, Номер 12(12), С. 2564 - 2564

Опубликована: Дек. 12, 2024

Resuspended particles from human activities can contribute to pathogen exposure via airborne fomite contamination in built environments. Studies investigating the dissemination of resuspended viruses are limited. The goal this study was explore viral after aerosolized resuspension on indoor flooring. Nylon carpet or wood flooring seeded with virus (MS2) laden dust then evaluated activities, i.e., walking and vacuuming. Statistically significant differences were found dispersal vacuuming (p-value = 5.8 × 10−6) 0.003, distance > 12 in/30 cm). Significant also between floor materials (p 2.09 10−5) 2.68 10−2). A quantitative microbial risk assessment (QMRA) scenario using Norovirus a single touch followed by hand-to-mouth indicated statistically difference associated carpet(p < 0.001). Infection risks 1 5 log10 greater for exposure. greatest reductions fomites seen across no-dust scenarios surfaces <30 cm More research is needed determine role plays transmission potentially infectious agents.

Язык: Английский

Changes in Respiratory Viruses’ Activity in Children During the COVID-19 Pandemic: A Systematic Review DOI Open Access
Marco Maglione, Vincenzo Tipo, Emiliano Barbieri

и другие.

Journal of Clinical Medicine, Год журнала: 2025, Номер 14(4), С. 1387 - 1387

Опубликована: Фев. 19, 2025

Background/Objectives: The impact of the coronavirus disease 2019 (COVID-19) pandemic on health was significant worldwide. measures adopted to limit spread virus had an indirect effect epidemiology other infectious diseases with similar mechanisms inter-human transmission. present literature review analyzed scenario pediatric acute respiratory infections in post-lockdown period compared pre-pandemic and lockdown periods. different patterns viruses' outbreaks were evaluated according type local COVID-19 restrictive pathogen. Methods: Relevant scientific published between March 2020 November 2024 identified by means electronic keyword searches PubMed, Scopus, Cochrane Library databases. Results: Worldwide implementation non-pharmacological public interventions aimed at limiting resulted a measurable circulation common viruses, significantly affecting their usual seasonality. Most viruses reduced activity during but returned or exceeded historical levels after discontinuation preventive measures. For many particularly syncytial virus, off-season increase reported. Conclusions: interventions, which effectively helped pandemic, relevant epidemiologic changes most viruses. Given seasonality clinical severity observed for some pathogens lockdown, possible future more severe epidemics should be expected.

Язык: Английский

Процитировано

2
Role of Gut Microbiota in Long COVID: Impact on Immune Function and Organ System Health DOI Open Access
Ankita Pathak, Devendra K. Agrawal

Archives of Microbiology & Immunology, Год журнала: 2025, Номер 9(1)

Опубликована: Янв. 1, 2025

SARS-CoV-2 infection has led to a range of long-lasting symptoms, collectively referred as long COVID. Current research highlights the critical role angiotensin-converting enzyme 2 (ACE2) in regulating gut microbiota diversity, vascular function, and homeostasis within renin-angiotensin system (RAS). ACE2 is utilized by virus enter host cells, but its downregulation following contributes dysbiosis RAS disruption. These imbalances have been linked COVID including joint pain, chest chronic cough, fatigue, brain fog, anxiety, depression, myalgia, peripheral neuropathy, memory difficulties, impaired attention. This review investigates dysregulation caused long-term effects it on various organ systems, musculoskeletal, neurological, renal, respiratory, cardiovascular systems. We explored bidirectional interactions between microbiota, immune these focusing how inflammation dysfunction observed symptoms. Understanding key for identifying effective therapeutic strategies interventional targets aimed at mitigating impact health improving patient outcomes.

Язык: Английский

Процитировано

1
The stability and elimination of mammalian enveloped and non-enveloped respiratory viruses in indoor air: Testing using a room-sized aerobiology chamber DOI Creative Commons
Bahram Zargar, Syed A. Sattar, Julie McKinney

и другие.

Journal of Virological Methods, Год журнала: 2025, Номер unknown, С. 115144 - 115144

Опубликована: Март 1, 2025

We assessed the viability of aerosolized human betacoronavirus OC43 (HCoV-OC43; ATCC VR-1558), rhinovirus-14 (RV-14; VR-284) and feline calicivirus (FCV; VR-782) as representative enveloped non-enveloped respiratory viruses mammals in indoor air under ambient conditions (relative humidity 50±10% temperature 22±2°C) using a room-sized (25 m3; 900 ft3) aerobiology chamber. All virus suspensions contained soil load to simulate presence body fluids they were separately into chamber six-jet Collison nebulizer. A muffin fan was used uniformly mix inside keep aerosols airborne. slit sampler with Petri plates containing 3% (wt./vol) gelatin collect samples. The liquefied an incubator assayed for infectious plaque-forming units (PFU). rates biological decay HCoV-OC43, RV-14 FCV 0.0052±0.00026, 0.0034±0.0027 0.0081±0.0031 (as log10 PFU/m3/min), respectively. also HEPA filter-based stand-alone purifier against experimentally device could demonstrate >3-log10 reductions three 46, 62 41minutes, Therefore, we can now investigate stability mammalian well decontamination technologies them field-relevant conditions.

Язык: Английский

Процитировано

0
Quantifying fomite hotspots and targeted hygiene impacts in a hotel lobby DOI Creative Commons

Manfred Spitzer,

Yoonhee Jung, Jonathan D. Sexton

и другие.

International Journal of Hygiene and Environmental Health, Год журнала: 2025, Номер 267, С. 114586 - 114586

Опубликована: Апрель 29, 2025

Язык: Английский

Процитировано

0
The Impact of an Air Sanitizer Spray on Risk of Virus Transmission by Aerosols Generated by Toilet Flushing DOI Creative Commons

Stephanie A. Boone,

Nicklous D Betts-Childress,

M. Khalid Ijaz

и другие.

American Journal of Infection Control, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Toilet flushing has been reported to result in pathogen contamination of restroom fomites and air. The goal this study was investigate the effectiveness an Air Sanitizing Spray on reducing cross-contamination air after toilet flushing. Bacteriophage MS2 added bowl water then flushed, were determined. Subsequently, procedure replicated determine interventional role using either Sanitizer or surface Disinfectant A 90 99.99% reduction occurred with use depending fomite location sanitizer spray duration. Use significantly reduced viral only seat spraying 8 seconds, but a 30 second 4 5 surfaces. also by 99% use. In contrast, when hard sprayed its impact post-flushing negligible. reduces potential airborne exposure from including risk norovirus transmission restroom.

Язык: Английский

Процитировано

0
Impact of Different Toilet Cleaning/Disinfecting Regimens on Reducing the Risk of Exposure to Toilet-Borne Pathogens in American Household Restrooms DOI Creative Commons

Stephanie A. Boone,

Nick D. Childress,

Norma Patricia Silva-Beltrán

и другие.

Hygiene, Год журнала: 2025, Номер 5(2), С. 22 - 22

Опубликована: Май 20, 2025

Restrooms are associated with the transmission of bacterial and viral illnesses. Disinfecting contaminated surfaces is reducing risk. The goal this study was to determine how cleaning/disinfecting frequency affects restroom pathogen contamination. Phase 1 intervention included cleaning toilet (the bowl, water, rim) using 9.5% w/w hydrochloric acid. 2 used fomite-specific products clean/disinfect additional surfaces, including vanity countertop, sink faucet handle, seat, flush floor, doorknob. A designated household member responsible for at beginning each interval. Fomite sample collection randomized, samples were tested heterotrophic, coliform, Escherichia coli bacteria after specified intervals: 1, 2, 3, or 7 days. greatest numbers largest reductions occurred on fomites three statistically significant difference found heterotrophic (p = 0.009), coliforms 0.10), E. 0.13) every quantitative microbial risk assessment (QMRA) estimated a >98% reduction in infection by norovirus an every-3-day cleaning/disinfection routine most heavily sites. Results indicate optimal twice weekly minimizing exposure pathogens.

Язык: Английский

Процитировано

0
Rapid virucidal activity of an air sanitizer against aerosolized MS2 and Phi6 phage surrogates for non-enveloped and enveloped vertebrate viruses, including SARS-CoV-2 DOI Creative Commons
M. Khalid Ijaz, Bahram Zargar, Raymond W. Nims

и другие.

Applied and Environmental Microbiology, Год журнала: 2024, Номер 91(1)

Опубликована: Дек. 6, 2024

An air sanitizer was evaluated using an aerobiology protocol, compliant with the U.S. Environmental Protection Agency's Air Sanitizer Guidelines, for virucidal activity against bacteriophages Phi6 and MS2 (used as surrogates enveloped non-enveloped human pathogenic viruses). The phages were suspended in a medium containing tripartite soil load simulating body fluids aerosolized six-jet Collison nebulizer enclosed 25 m3 chamber at 22 ± 2°C 50 10% relative humidity. sprayed into 30 s. Viable captured directly, real time, on host bacterial lawns slit-to-agar sampler. Reductions viable phage concentration ≥3.0 log10 (99.9%) observed after mean exposure of 3.6 min Phi6, suggesting efficacy viruses (e.g., SARS-CoV-2, influenza, RSV), ~10.6 MS2, noroviruses rhinoviruses). This targeted sanitization approach represents important non-pharmaceutical public health intervention airborne viral pathogens.IMPORTANCEAirborne are implicated transmission indoors respiratory enteric infections. sanitizers represent to mitigate risk such transmission. We have developed method that is now ASTM International standard (ASTM E3273-21) well test protocol approved by EPA evaluate sanitizing sprays inactivating bacteriophage viruses, respectively). individually room-sized maintained ambient temperature SARS-CoV-2; influenza; data suggest utility mitigating indoor during pandemics outbreaks.

Язык: Английский

Процитировано

2
Characterization of size-resolved aerosol hygroscopicity and liquid water content in Nanjing of the Yangtze River Delta DOI
Youling Jiang, Yan Ma, Jun Zheng

и другие.

Journal of Environmental Sciences, Год журнала: 2024, Номер 151, С. 26 - 41

Опубликована: Март 28, 2024

Язык: Английский

Процитировано

1
Resuspension and Dissemination of MS2 Virus from Flooring After Human Activities in Built Environment: Impact of Dust Particles DOI Creative Commons

Stephanie A. Boone,

M. Khalid Ijaz, Julie McKinney

и другие.

Microorganisms, Год журнала: 2024, Номер 12(12), С. 2564 - 2564

Опубликована: Дек. 12, 2024

Resuspended particles from human activities can contribute to pathogen exposure via airborne fomite contamination in built environments. Studies investigating the dissemination of resuspended viruses are limited. The goal this study was explore viral after aerosolized resuspension on indoor flooring. Nylon carpet or wood flooring seeded with virus (MS2) laden dust then evaluated activities, i.e., walking and vacuuming. Statistically significant differences were found dispersal vacuuming (p-value = 5.8 × 10−6) 0.003, distance > 12 in/30 cm). Significant also between floor materials (p 2.09 10−5) 2.68 10−2). A quantitative microbial risk assessment (QMRA) scenario using Norovirus a single touch followed by hand-to-mouth indicated statistically difference associated carpet(p < 0.001). Infection risks 1 5 log10 greater for exposure. greatest reductions fomites seen across no-dust scenarios surfaces <30 cm More research is needed determine role plays transmission potentially infectious agents.

Язык: Английский

Процитировано

1