A necrotizing toxin enables Pseudomonas syringae infection across evolutionarily divergent plants
Cell Host & Microbe,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 1, 2024
Язык: Английский
Rapid detection of RNase‐based self‐incompatibility in Lysimachia monelli (Primulaceae)
American Journal of Botany,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 13, 2025
Abstract
Premise
Primroses
famously
employ
a
system
that
simultaneously
expresses
distyly
and
filters
out
self‐pollen.
Other
species
in
the
Primulaceae
family,
including
Lysimachia
monelli
(blue
pimpernel),
also
express
self‐incompatibility
(SI),
but
involving
with
distinct
features
an
unknown
molecular
genetic
basis.
Methods
We
utilize
candidate‐based
transcriptome
sequencing
(RNA‐seq)
approach,
relying
on
candidate
T2/S‐RNase
Class
III
S‐linked
F‐box‐motif‐containing
genes
harnessing
unusual
evolutionary
of
SI,
to
examine
whether
RNase‐based
mechanism
underlies
SI
L.
.
term
this
approach
"SI
detection
RNA‐seq"
(SIDR).
Results
The
results
sequencing,
crossing,
population
genetics,
each
support
causal
association
linking
recovered
genotypes
phenotypes.
finding
(Ericales)
all
cements
long‐held
view
was
present
ancestral
pentapetal
eudicot,
whose
descendants
now
comprise
two‐thirds
angiosperms.
It
significantly
narrows
plausible
maximum
age
for
heterostyly
evolution
within
family.
Conclusions
SIDR
is
powerful,
flexible,
inexpensive,
most
critically
enables
work
often‐neglected
species.
may
be
used
or
without
close
enormous
gaps
understanding
basis
history
breeding
evolution.
Язык: Английский
Transcriptome data from silica-preserved leaf tissue reveal gene flow patterns in a Caribbean bromeliad
Annals of Botany,
Год журнала:
2024,
Номер
133(3), С. 459 - 472
Опубликована: Янв. 4, 2024
Abstract
Background
and
Aims
Transcriptome
sequencing
is
a
cost-effective
approach
that
allows
researchers
to
study
broad
range
of
questions.
However,
preserve
RNA
for
transcriptome
sequencing,
tissue
often
kept
in
special
conditions,
such
as
immediate
ultracold
freezing.
Here,
we
demonstrate
can
be
obtained
from
6-month-old,
field-collected
samples
stored
silica
gel
at
room
temperature.
Using
these
transcriptomes,
explore
the
evolutionary
relationships
genus
Pitcairnia
(Bromeliaceae)
Dominican
Republic
infer
barriers
gene
flow.
Methods
We
extracted
silica-dried
leaf
19
individuals
collected
across
Republic.
used
series
macro-
micro-evolutionary
approaches
examine
patterns
flow
among
individuals.
Key
Results
produced
high-quality
transcriptomes
material
demonstrated
on
island
match
geography
more
closely
than
species
delimitation
methods.
A
population
genetic
examination
indicates
combination
ecological
geographical
features
presents
Pitcairnia.
Conclusions
Transcriptomes
silica-preserved
tissue.
The
diversity
populations
does
not
warrant
classification
separate
species,
but
contains
several
flow,
notably
Cordillera
Central
mountain
range.
Язык: Английский
A Necrotizing Toxin Promotes Pseudomonas syringae Infection Across Evolutionarily Divergent Plant Lineages
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 19, 2024
ABSTRACT
The
Pseudomonas
syringae
species
complex
harbors
a
diverse
range
of
plant
pathogenic
bacteria.
While
much
the
current
understanding
P.
is
centered
on
interactions
with
flowering
plants,
less
known
about
infection
in
evolutionarily
divergent
non-flowering
lineages.
Here,
we
took
comparative
evolutionary
approach
to
understand
how
infects
distantly
related
plants.
We
identify
broad
host
isolates
causing
significant
disease
liverwort
Marchantia
polymorpha
,
fern
Ceratopteris
richardii
and
Nicotiana
benthamiana
which
last
shared
common
ancestor
over
500
million
years
ago.
demonstrate
that
phytotoxin
enriched
belonging
phylogroup
2
clade
are
particularly
virulent
relying
combination
type-3
secreted
effector
proteins
lipopeptide
syringomycin.
application
purified
syringomycin
promotes
necrosis
tissues
activates
conserved
genes
associated
redox
regulation
cell
death.
Toxin-deficient
phylogroups
normally
unable
infect
thalli
exhibit
enhanced
bacterial
growth
when
supplemented
exogenous
syringomycin,
further
highlighting
its
role
as
host-range
defining
factor
.
Collectively
our
research
reveals
key
for
promoting
colonization,
works
concert
antagonize
an
exceptionally
wide
spectrum
land
Язык: Английский