The sponge holobiont in a changing ocean: from microbes to ecosystems

Microbiome, Год журнала: 2018, Номер 6(1)

Опубликована: Март 9, 2018

The recognition that all macroorganisms live in symbiotic association with microbial communities has opened up a new field biology. Animals, plants, and algae are now considered holobionts, complex ecosystems consisting of the host, microbiota, interactions among them. Accordingly, ecological concepts can be applied to understand host-derived processes govern dynamics interactive networks within holobiont. In marine systems, holobionts further integrated into larger more ecosystems, concept referred as "nested ecosystems." this review, we discuss dynamic interact at multiple scales respond environmental change. We focus on symbiosis sponges their communities—a resulted one most diverse environment. recent years, sponge microbiology remarkably advanced terms curated databases, standardized protocols, information functions microbiota. Like Russian doll, these translated holobiont impact surrounding ecosystem. For example, sponge-associated metabolisms, fueled by high filtering capacity substantially affect biogeochemical cycling key nutrients like carbon, nitrogen, phosphorous. Since increasingly threatened anthropogenic stressors jeopardize stability ecosystem, link between perturbations, dysbiosis, diseases. Experimental studies suggest community composition is tightly linked health, but whether dysbiosis cause or consequence collapse remains unresolved. Moreover, potential role microbiome mediating for acclimate adapt change unknown. Future should aim identify mechanisms underlying scales, from develop management strategies preserve provided our present future oceans.

Язык: Английский

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Predicting the HMA-LMA Status in Marine Sponges by Machine Learning

Frontiers in Microbiology, Год журнала: 2017, Номер 8

Опубликована: Май 8, 2017

The dichotomy between high microbial abundance (HMA) and low (LMA) sponges has been observed in sponge-microbe symbiosis, although the extent of this pattern remains poorly unknown. We characterized differences microbiomes HMA (n = 19) LMA 17) (575 specimens) present Sponge Microbiome Project. were associated with richer more diverse than sponges, as indicated by comparison alpha diversity metrics. Microbial community structures differed considering Operational Taxonomic Units (OTU) abundances across taxonomic levels, from phylum to species. largest proportion microbiome variation was explained host identity. Several phyla, classes, OTUs found differentially abundant either group, which considered "HMA indicators" "LMA indicators." Machine learning algorithms (classifiers) trained predict HMA-LMA status sponges. Among nine different classifiers, higher performances achieved Random Forest class abundances. optimized parameters predicted additional 135 sponge species (1,232 without a priori knowledge. These grouped four clusters, two composed consistently 44) 74). In summary, our analyses shown distinct features communities prediction based on profiles demonstrates application machine explore patterns host-associated communities.

Язык: Английский

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Biodiversity and Functional Ecology of Mesophotic Coral Reefs

Annual Review of Ecology Evolution and Systematics, Год журнала: 2018, Номер 49(1), С. 49 - 71

Опубликована: Ноя. 2, 2018

Mesophotic coral reefs, currently defined as deep reefs between 30 and 150 m, are linked physically biologically to their shallow water counterparts, have the potential be refuges for reef taxa such sponges, might a source of larvae that could contribute resiliency reefs. found worldwide, but most undescribed understudied. Here, we review our current knowledge mesophotic functional ecology it relates geomorphology, changes in abiotic environment along depth gradients, trophic ecology, reproduction, connectivity depths. Understanding them is now primary focus many studies worldwide degradation ecosystem services they provide, continues unabated.

Язык: Английский

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Emerging Sponge Models of Animal-Microbe Symbioses

Frontiers in Microbiology, Год журнала: 2016, Номер 7

Опубликована: Дек. 23, 2016

Sponges have a significant impact on marine benthic communities, they are of biotechnological interest owing to their production bioactive natural compounds, and promise provide insights into conserved mechanisms host-microbe interactions in basal metazoans. The variability sponge-microbe associations across species environments provides meaningful ecological evolutionary framework investigate animal-microbial symbiosis through experimentation the field also aquaria. In addition, next-generation sequencing technologies shed light genomic repertoire sponge host revealed metabolic capacities symbiotic lifestyle features microbiota. However, our understanding is still its infancy. Here, we discuss potential limitations as emerging models for animal-associated

Язык: Английский

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Compositional and Quantitative Insights Into Bacterial and Archaeal Communities of South Pacific Deep-Sea Sponges (Demospongiae and Hexactinellida)

Frontiers in Microbiology, Год журнала: 2020, Номер 11

Опубликована: Апрель 24, 2020

In the present study, we profiled bacterial and archaeal communities from thirteen phylogenetically diverse deep-sea sponge species (Demospongiae Hexactinellida) South Pacific by 16S rRNA-gene amplicon sequencing. Additionally, associated bacteria archaea were quantified real-time qPCR. Our results show that sponges are mostly host-species specific similar to what has been observed for shallow-water demosponges. The community structures different in they almost completely dominated a single family, which ammonia-oxidizing genera within Nitrosopumilaceae. Remarkably, individual (rather than sponge-species), this observation applies both hexactinellids Finally, numbers, as detected quantitative PCR, up three orders of magnitude higher sponges, highlighting importance general.

Язык: Английский

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Prokaryotic Diversity and Community Patterns in Antarctic Continental Shelf Sponges

Frontiers in Marine Science, Год журнала: 2019, Номер 6

Опубликована: Июнь 5, 2019

Marine sponges (Phylum Porifera) are globally distributed within marine and freshwater ecosystems. In addition, host dense diverse prokaryotic communities, which potential sources of novel bioactive metabolites other complex compounds. Those sponge-derived natural products can span a broad spectrum bioactivities, from antibacterial antifungal to antitumor antiviral However, most analyses concerning sponge-associated prokaryotes have mainly focused on conveniently accessible relatively shallow sampling locations for sponges. Hence, knowledge community composition, host-relatedness biotechnological associations in temperate cold-water greater depths (mesophotic mesopelagic zones) is still scarce. Therefore, we analyzed the diversity four phylogenetically divergent sponge taxa mesophotic Antarctic shelf at different region South Shetland Islands using 16S rRNA gene amplicon-based sequencing. predicted functional profiles applying Tax4Fun metagenomic data estimate their capability possible roles as We found indications that cold deep-water exhibit host-specific despite sites depths. Functional prediction analysis suggests associated may enhance biodegradation processes xenobiotics involvement biosynthesis secondary metabolites.

Язык: Английский

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Diversity and shifts of the bacterial community associated with Baikal sponge mass mortalities

PLoS ONE, Год журнала: 2019, Номер 14(3), С. e0213926 - e0213926

Опубликована: Март 28, 2019

The disease of freshwater sponges was first discovered in 2011, when pink samples were found the Central Basin Lake Baikal. Subsequently, visible signs have changed, and now appear with various symptoms damage to body, such as discoloration, tissue necrosis, formation brown patches dirty-purple biofilms on some branches. These are accompanied by mass death sponges. We identified differences microbiomes sequencing 16S rRNA genes changes consortium microorganisms Baikal that observed imbalance studied microbial communities diseased is caused several different conditionally pathogenic increase their negative effect acting together concert, which leads photosynthetic microalgae Sponges an important component coastal communities, massive loss can obviously affect structure benthic purity water.

Язык: Английский

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Phylogeny resolved, metabolism revealed: functional radiation within a widespread and divergent clade of sponge symbionts

The ISME Journal, Год журнала: 2020, Номер 15(2), С. 503 - 519

Опубликована: Окт. 3, 2020

Язык: Английский

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How does heat stress affect sponge microbiomes? Structure and resilience of microbial communities of marine sponges from different habitats

Frontiers in Marine Science, Год журнала: 2023, Номер 9

Опубликована: Янв. 19, 2023

Introduction Sponges are key components of marine benthic communities, providing many ecosystem functions and establishing close relationships with microorganisms, conforming the holobiont. These symbiotic microbiotas seem to be host species-specific highly diverse, playing roles in their sponge host. The effects elevated seawater temperature on sponges microbiota still poorly known, whether from polar areas more sensitive these impacts respect temperate tropical species is totally unknown. Methods We analyzed microbiomes different natural habitat after exposure heat stress aquaria by 16S rRNA amplicon sequencing (1) characterize covering a latitudinal gradient (polar, environments), (2) asses thermal microbial communities. Results Bacterial communities’ structure was also surrounding seawater. core microbiome maintained most stress, although they would recover normal conditions previous remains yet further investigated. observed increased abundances transient bacteria unknown origin exposed stress. Discussion Some may opportunistic that benefit stress-associated dysregulation occupying new niches According our results, Antarctic waters could resilient than sponges. Both composition changes produced quite species-specific, thus, depend species. Under global change scenario, will probably those suffering therefore dramatic for ecosystems since fundamental part them.

Язык: Английский

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Host-specific assembly of sponge-associated prokaryotes at high taxonomic ranks

Scientific Reports, Год журнала: 2017, Номер 7(1)

Опубликована: Май 24, 2017

Abstract Sponges (Porifera) are abundant and diverse members of benthic filter feeding communities in most marine ecosystems, from the deep sea to tropical reefs. A characteristic feature is associated dense prokaryotic community present within sponge mesohyl. Previous molecular genetic studies revealed importance host identity for composition sponge-associated microbiota. However, little known whether host-specific patterns observed at 97% 16S rRNA gene sequence similarity consistent high taxonomic ranks (from genus phylum level). In study, we investigated structure variation 24 specimens (seven taxa) three seawater samples Sweden. Results show that resemblance different with operational unit level.

Язык: Английский

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