Research in Biotechnology and Environmental Science,
Год журнала:
2024,
Номер
3(2), С. 18 - 22
Опубликована: Июнь 25, 2024
Introduction:
Microbes
of
sponges
have
diverse
associations,
including
true
symbiosis.
Sponges,
being
evolutionarily
ancient
sessile
filter
feeders,
host
and
abundant
microbial
species
that
play
crucial
roles
in
metabolism.
Although
the
symbionts
are
widely
distributed
within
organism
(up
to
40%
their
volume),
ecological
relationships
interactions
between
bacteria
sponge
remain
largely
unexplored
for
many
species.
The
present
study
was
one
first
attempts
isolate
symbiotic
from
Raspaciona
aculeata.
Materials
Methods:
After
isolation
on
marine
agar
medium,
isolates
were
characterized
different
colony
morphology.
16S
rDNA
taxonomic
analysis
carried
out
isolates.
Results:
Following
an
incubation
period
two
weeks
at
25°C,
only
13
bacterial
strains
isolated
with
a
very
low
rate
genetic
biodiversity.
All
belonged
Gammaproteobacteria
class
(Pseudomonadaceae
family),
except
(isolate
AL-18ra)
belonging
Bacilli
(Bacillaceae
family).
Conclusion:
obtained
results
great
importance
advancing
understanding
symbiosis
phenomena
aculeata
its
bioapplication
potential.
Frontiers in Microbiology,
Год журнала:
2020,
Номер
11
Опубликована: Апрель 24, 2020
In
the
present
study,
we
profiled
bacterial
and
archaeal
communities
from
thirteen
phylogenetically
diverse
deep-sea
sponge
species
(Demospongiae
Hexactinellida)
South
Pacific
by
16S
rRNA-gene
amplicon
sequencing.
Additionally,
associated
bacteria
archaea
were
quantified
real-time
qPCR.
Our
results
show
that
sponges
are
mostly
host-species
specific
similar
to
what
has
been
observed
for
shallow-water
demosponges.
The
community
structures
different
in
they
almost
completely
dominated
a
single
family,
which
ammonia-oxidizing
genera
within
Nitrosopumilaceae.
Remarkably,
individual
(rather
than
sponge-species),
this
observation
applies
both
hexactinellids
Finally,
numbers,
as
detected
quantitative
PCR,
up
three
orders
of
magnitude
higher
sponges,
highlighting
importance
general.
Environmental Microbiology,
Год журнала:
2021,
Номер
23(3), С. 1638 - 1655
Опубликована: Янв. 6, 2021
Summary
Kelps
are
dominant
primary
producers
in
temperate
coastal
ecosystems.
Large
amounts
of
kelp
biomass
can
be
exported
to
the
seafloor
during
algal
growth
cycle
or
following
storms,
creating
new
ecological
niches
for
associated
microbiota.
Here,
we
investigated
bacterial
community
with
Laminaria
hyperborea
its
accumulation
and
degradation
on
seafloor.
Kelp
tissue,
seawater
sediment
were
sampled
a
6‐month
situ
experiment
simulating
detritus
accumulation.
Evaluation
epiphytic
abundance,
structure,
taxonomic
composition
predicted
functional
profiles
evidenced
biphasic
succession.
Initially,
genera
(
Hellea
,
Litorimonas
Granulosicoccus
)
showed
rapid
drastic
decrease
sequence
probably
outcompeted
by
polysaccharide‐degraders
such
as
Bacteroidia
members
which
responded
within
4
weeks.
Acidimicrobiia
especially
Sva0996
marine
group,
colonized
degrading
after
11
These
secondary
colonizers
could
act
opportunistic
scavenger
bacteria
assimilating
substrates
exposed
early
degraders.
In
parallel,
modified
communities
underlying
sediment,
notably
favouring
anaerobic
taxa
potentially
involved
sulfur
nitrogen
cycles.
Overall,
this
study
provides
insights
into
an
important
link
trophic
chains.
Scientific Reports,
Год журнала:
2022,
Номер
12(1)
Опубликована: Март 1, 2022
Abstract
Marine
sponges
(phylum
Porifera)
are
leading
organisms
for
the
discovery
of
bioactive
compounds
from
nature.
Their
often
rich
and
species-specific
microbiota
is
hypothesised
to
be
producing
many
these
compounds.
Yet,
environmental
influences
on
sponge-associated
compound
production
remain
elusive.
Here,
we
investigated
changes
metabolomes
in
along
a
depth
range
1232
m.
Using
16S
rRNA
gene
amplicon
sequencing
untargeted
metabolomics,
assessed
prokaryotic
chemical
diversities
three
deep-sea
sponge
species:
Geodia
barretti
,
Stryphnus
fortis
Weberella
bursa
.
Both
communities
metabolome
varied
significantly
with
depth,
which
hypothesized
effect
different
water
masses.
Up
35.5%
microbial
ASVs
(amplicon
sequence
variants)
showed
significant
while
phylum-level
composition
host
microbiome
remained
unchanged.
The
relative
quantities
known
increasing
or
decreasing
strongly.
Other
metabolites
varying
were
compatible
solutes
regulating
osmolarity
cells.
Correlations
between
community
G.
suggested
members
Acidobacteria,
Proteobacteria,
Chloroflexi,
an
unclassified
prokaryote
as
potential
producers.
Scientific Reports,
Год журнала:
2023,
Номер
13(1)
Опубликована: Апрель 8, 2023
Abstract
The
potential
of
sponge-derived
chemicals
for
pharmaceutical
applications
remains
largely
unexploited
due
to
limited
available
biomass.
Although
many
have
attempted
culture
marine
sponge
cells
in
vitro
create
a
scalable
production
platform
such
biopharmaceuticals,
these
efforts
been
mostly
unsuccessful.
We
recently
showed
that
Geodia
barretti
could
divide
rapidly
M1
medium.
In
this
study
we
established
the
first
continuous
cell
line,
originating
from
G.
.
cultured
OpM1
medium,
modification
M1,
grew
more
and
higher
density
than
M1.
Cells
reached
1.74
population
doublings
after
30
min,
twofold
already
rapid
growth
rate
0.74
min
maximum
number
increased
5
at
least
98
OpM1.
Subcultured
be
cryopreserved
used
inoculate
new
cultures.
With
results,
overcome
major
obstacle
has
blocked
path
producing
biopharmaceuticals
with
industrial
scale
decades.
Microbial Biotechnology,
Год журнала:
2021,
Номер
14(3), С. 1159 - 1170
Опубликована: Март 8, 2021
Summary
Marine
photosynthetic
microalgae
are
ubiquitously
associated
with
bacteria
in
nature.
However,
the
influence
of
these
on
algal
cultures
bioreactors
is
still
largely
unknown.
In
this
study,
eighteen
different
bacterial
strains
were
isolated
from
Nannochloropsis
sp.
CCAP211/78
two
outdoor
pilot‐scale
tubular
photobioreactors.
The
majority
isolates
was
affiliated
classes
Alphaproteobacteria
and
Flavobacteriia
.
To
assess
impact
growth
CCAP211/78,
24‐well
plates
coupled
custom‐made
LED
boxes
used
to
simultaneously
compare
replicate
axenic
microalgal
addition
individual
isolates.
Co‐culturing
demonstrated
distinct
responses,
which
shows
that
technique
we
developed
an
efficient
method
for
screening
harmful/beneficial
bacteria.
Two
tested
strains,
namely
a
strain
Maritalea
porphyrae
(DMSP31)
Labrenzia
aggregata
(YP26),
significantly
enhanced
14%
12%
increase
chlorophyll
concentration,
respectively,
whereas
flavobacterial
YP206
greatly
inhibited
microalga
28%
reduction
concentration.
Our
study
suggests
production
systems
represent
‘natural’
source
isolate
microorganisms
can
either
benefit
or
harm
cultures.
Marine Drugs,
Год журнала:
2021,
Номер
19(7), С. 353 - 353
Опубликована: Июнь 22, 2021
This
study
aimed
to
assess
the
diversity
and
antimicrobial
activity
of
cultivable
bacteria
associated
with
Vietnamese
sponges.
In
total,
460
bacterial
isolates
were
obtained
from
18
marine
Of
these,
58.3%
belonged
Proteobacteria,
16.5%
Actinobacteria,
18.0%
Firmicutes,
7.2%
Bacteroidetes.
At
genus
level,
isolated
strains
55
genera,
which
several
such
as
Bacillus,
Pseudovibrio,
Ruegeria,
Vibrio,
Streptomyces,
most
predominant.
Culture
media
influenced
composition,
whereas,
different
sponge
species,
similar
recovered.
Interestingly,
there
was
little
overlap
composition
sponges
when
taxa
compared
cultivation-independent
data.
Subsequent
assays
showed
that
90
exhibited
against
at
least
one
seven
indicator
microorganisms.
From
culture
broth
strain
strongest
(Bacillus
sp.
M1_CRV_171),
four
secondary
metabolites
identified,
including
cyclo(L-Pro-L-Tyr)
(1),
macrolactin
A
(2),
H
(3),
15,17-epoxy-16-hydroxy
(4).
compounds
2-4
a
broad
spectrum
reference
Frontiers in Microbiology,
Год журнала:
2021,
Номер
12
Опубликована: Ноя. 10, 2021
The
application
of
high-throughput
microbial
community
profiling
as
well
“omics”
approaches
unveiled
high
diversity
and
host-specificity
bacteria
associated
with
marine
sponges,
which
are
renowned
for
their
wide
range
bioactive
natural
products.
However,
exploration
exploitation
compounds
from
sponge-associated
have
been
limited
because
the
majority
remains
recalcitrant
to
cultivation.
In
this
review,
we
(i)
discuss
recent/novel
cultivation
techniques
that
used
isolate
bacteria,
(ii)
provide
an
overview
isolated
sponges
until
2017
culture
conditions
identify
not
yet
cultured
(iii)
outline
promising
strategies
cultivating
uncultivated
in
future.
Despite
intensive
attempts,
obtained
through
much
lower
than
seen
cultivation-independent
methods,
is
particularly
noticeable
those
taxa
were
previously
marked
“sponge-specific”
“sponge-enriched.”
This
poses
urgent
need
more
efficient
methods.
Refining
media
based
on
information
metagenomic
datasets
under
simulated
most
wanted
bacteria.
Frontiers in Marine Science,
Год журнала:
2021,
Номер
8
Опубликована: Июль 20, 2021
Marine
sponges
have
been
successful
in
their
expansion
across
diverse
ecological
niches
around
the
globe.
Pioneering
work
attributed
this
success
to
both
a
well-developed
aquiferous
system
that
allowed
for
efficient
filter
feeding
on
suspended
organic
matter
and
presence
of
microbial
symbionts
can
supplement
host
heterotrophic
with
photosynthate
or
dissolved
carbon.
We
now
know
sponge-microbe
interactions
are
host-specific,
highly
nuanced,
provide
nutritional
benefits
sponge.
Despite
these
advances
field,
many
current
hypotheses
pertaining
evolution
overly
generalized;
over-simplifications
limit
our
understanding
evolutionary
processes
shaping
symbioses
how
they
contribute
modern
coral
reefs.
To
highlight
state
knowledge
we
start
seminal
papers
review
contemporary
using
higher
resolution
techniques
has
complemented
challenged
early
hypotheses.
outline
different
schools
thought
by
discussing
evidence
symbiont
contribution
divergence
convergence,
specificity
plasticity,
allopatric
sympatric
speciation.
Based
synthesis,
conclude
pressures
complex,
influences
from
external
(nutrient
limitation
competition)
internal
(fitness
trade-offs
constraints)
factors.
recent
controversies
place
into
broader
framework.
Finally,
propose
areas
future
research
believe
will
lead
important
new
developments
field.
Frontiers in Marine Science,
Год журнала:
2020,
Номер
6
Опубликована: Янв. 7, 2020
Archaea
is
a
poorly
studied
domain
associated
with
sponges.
Many
questions
that
have
been
addressed
for
bacteria
still
remain
largely
unknown
archaea.
In
this
study,
we
analysed
the
archaeal
communities
of
17
tropical
sponge
species
from
Nha
Trang
Bay
(Vietnam)
using
archaea
specific
primers.
We
recorded
patterns
diversity
and
spatial
stability
these
microbial
compared
results
obtained
bacterial
communities,
already
reported
in
our
previous
study.
study
species,
Shannon
was
always
lower
than
communities.
The
differences
alpha
as
well
presence
indicator
phyla
High
Microbial
Abundance
(HMA)
Low
(LMA)
could
not
be
confirmed
Host
identity
main
factor
structuring
assemblages.
Sponge
core
formed
by
few
but
very
abundant
ZOTUs,
which
contribute
high
proportion
to
relative
abundance.
inclusion
sequences
into
phylogenetic
trees
allowed
finding
out
whether
or
they
belonged
so-called
clusters
(SC).
Our
showed
most
Thaumarchaeota
Euryarchaeota
were
more
closely
related
environmental
samples
clusters,
suggesting
might
acquired
seawater,
need
verified.
However,
representatives
Woesarchaeota,
major
members
microbiome
two
monophyletic
tree,
distantly
any
known
sequence.