Compositional and Quantitative Insights Into Bacterial and Archaeal Communities of South Pacific Deep-Sea Sponges (Demospongiae and Hexactinellida)

Frontiers in Microbiology, Год журнала: 2020, Номер 11

Опубликована: Апрель 24, 2020

In the present study, we profiled bacterial and archaeal communities from thirteen phylogenetically diverse deep-sea sponge species (Demospongiae Hexactinellida) South Pacific by 16S rRNA-gene amplicon sequencing. Additionally, associated bacteria archaea were quantified real-time qPCR. Our results show that sponges are mostly host-species specific similar to what has been observed for shallow-water demosponges. The community structures different in they almost completely dominated a single family, which ammonia-oxidizing genera within Nitrosopumilaceae. Remarkably, individual (rather than sponge-species), this observation applies both hexactinellids Finally, numbers, as detected quantitative PCR, up three orders of magnitude higher sponges, highlighting importance general.

Язык: Английский

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Accumulation of detached kelp biomass in a subtidal temperate coastal ecosystem induces succession of epiphytic and sediment bacterial communities

Environmental Microbiology, Год журнала: 2021, Номер 23(3), С. 1638 - 1655

Опубликована: Янв. 6, 2021

Summary Kelps are dominant primary producers in temperate coastal ecosystems. Large amounts of kelp biomass can be exported to the seafloor during algal growth cycle or following storms, creating new ecological niches for associated microbiota. Here, we investigated bacterial community with Laminaria hyperborea its accumulation and degradation on seafloor. Kelp tissue, seawater sediment were sampled a 6‐month situ experiment simulating detritus accumulation. Evaluation epiphytic abundance, structure, taxonomic composition predicted functional profiles evidenced biphasic succession. Initially, genera ( Hellea , Litorimonas Granulosicoccus ) showed rapid drastic decrease sequence probably outcompeted by polysaccharide‐degraders such as Bacteroidia members which responded within 4 weeks. Acidimicrobiia especially Sva0996 marine group, colonized degrading after 11 These secondary colonizers could act opportunistic scavenger bacteria assimilating substrates exposed early degraders. In parallel, modified communities underlying sediment, notably favouring anaerobic taxa potentially involved sulfur nitrogen cycles. Overall, this study provides insights into an important link trophic chains.

Язык: Английский

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Oceanographic setting influences the prokaryotic community and metabolome in deep-sea sponges

Scientific Reports, Год журнала: 2022, Номер 12(1)

Опубликована: Март 1, 2022

Abstract Marine sponges (phylum Porifera) are leading organisms for the discovery of bioactive compounds from nature. Their often rich and species-specific microbiota is hypothesised to be producing many these compounds. Yet, environmental influences on sponge-associated compound production remain elusive. Here, we investigated changes metabolomes in along a depth range 1232 m. Using 16S rRNA gene amplicon sequencing untargeted metabolomics, assessed prokaryotic chemical diversities three deep-sea sponge species: Geodia barretti , Stryphnus fortis Weberella bursa . Both communities metabolome varied significantly with depth, which hypothesized effect different water masses. Up 35.5% microbial ASVs (amplicon sequence variants) showed significant while phylum-level composition host microbiome remained unchanged. The relative quantities known increasing or decreasing strongly. Other metabolites varying were compatible solutes regulating osmolarity cells. Correlations between community G. suggested members Acidobacteria, Proteobacteria, Chloroflexi, an unclassified prokaryote as potential producers.

Язык: Английский

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First continuous marine sponge cell line established

Scientific Reports, Год журнала: 2023, Номер 13(1)

Опубликована: Апрель 8, 2023

Abstract The potential of sponge-derived chemicals for pharmaceutical applications remains largely unexploited due to limited available biomass. Although many have attempted culture marine sponge cells in vitro create a scalable production platform such biopharmaceuticals, these efforts been mostly unsuccessful. We recently showed that Geodia barretti could divide rapidly M1 medium. In this study we established the first continuous cell line, originating from G. . cultured OpM1 medium, modification M1, grew more and higher density than M1. Cells reached 1.74 population doublings after 30 min, twofold already rapid growth rate 0.74 min maximum number increased 5 at least 98 OpM1. Subcultured be cryopreserved used inoculate new cultures. With results, overcome major obstacle has blocked path producing biopharmaceuticals with industrial scale decades.

Язык: Английский

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Different co‐occurring bacteria enhance or decrease the growth of the microalga Nannochloropsis sp. CCAP211/78

Microbial Biotechnology, Год журнала: 2021, Номер 14(3), С. 1159 - 1170

Опубликована: Март 8, 2021

Summary Marine photosynthetic microalgae are ubiquitously associated with bacteria in nature. However, the influence of these on algal cultures bioreactors is still largely unknown. In this study, eighteen different bacterial strains were isolated from Nannochloropsis sp. CCAP211/78 two outdoor pilot‐scale tubular photobioreactors. The majority isolates was affiliated classes Alphaproteobacteria and Flavobacteriia . To assess impact growth CCAP211/78, 24‐well plates coupled custom‐made LED boxes used to simultaneously compare replicate axenic microalgal addition individual isolates. Co‐culturing demonstrated distinct responses, which shows that technique we developed an efficient method for screening harmful/beneficial bacteria. Two tested strains, namely a strain Maritalea porphyrae (DMSP31) Labrenzia aggregata (YP26), significantly enhanced 14% 12% increase chlorophyll concentration, respectively, whereas flavobacterial YP206 greatly inhibited microalga 28% reduction concentration. Our study suggests production systems represent ‘natural’ source isolate microorganisms can either benefit or harm cultures.

Язык: Английский

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Diversity and Antimicrobial Activity of Vietnamese Sponge-Associated Bacteria

Marine Drugs, Год журнала: 2021, Номер 19(7), С. 353 - 353

Опубликована: Июнь 22, 2021

This study aimed to assess the diversity and antimicrobial activity of cultivable bacteria associated with Vietnamese sponges. In total, 460 bacterial isolates were obtained from 18 marine Of these, 58.3% belonged Proteobacteria, 16.5% Actinobacteria, 18.0% Firmicutes, 7.2% Bacteroidetes. At genus level, isolated strains 55 genera, which several such as Bacillus, Pseudovibrio, Ruegeria, Vibrio, Streptomyces, most predominant. Culture media influenced composition, whereas, different sponge species, similar recovered. Interestingly, there was little overlap composition sponges when taxa compared cultivation-independent data. Subsequent assays showed that 90 exhibited against at least one seven indicator microorganisms. From culture broth strain strongest (Bacillus sp. M1_CRV_171), four secondary metabolites identified, including cyclo(L-Pro-L-Tyr) (1), macrolactin A (2), H (3), 15,17-epoxy-16-hydroxy (4). compounds 2-4 a broad spectrum reference

Язык: Английский

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Bacteria Cultivated From Sponges and Bacteria Not Yet Cultivated From Sponges—A Review

Frontiers in Microbiology, Год журнала: 2021, Номер 12

Опубликована: Ноя. 10, 2021

The application of high-throughput microbial community profiling as well “omics” approaches unveiled high diversity and host-specificity bacteria associated with marine sponges, which are renowned for their wide range bioactive natural products. However, exploration exploitation compounds from sponge-associated have been limited because the majority remains recalcitrant to cultivation. In this review, we (i) discuss recent/novel cultivation techniques that used isolate bacteria, (ii) provide an overview isolated sponges until 2017 culture conditions identify not yet cultured (iii) outline promising strategies cultivating uncultivated in future. Despite intensive attempts, obtained through much lower than seen cultivation-independent methods, is particularly noticeable those taxa were previously marked “sponge-specific” “sponge-enriched.” This poses urgent need more efficient methods. Refining media based on information metagenomic datasets under simulated most wanted bacteria.

Язык: Английский

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Biodegradation of petroleum oil using a constructed nonpathogenic and heavy metal-tolerant bacterial consortium isolated from marine sponges

Journal of environmental chemical engineering, Год журнала: 2022, Номер 10(6), С. 108752 - 108752

Опубликована: Окт. 12, 2022

Язык: Английский

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Sponge–Microbe Interactions on Coral Reefs: Multiple Evolutionary Solutions to a Complex Environment

Frontiers in Marine Science, Год журнала: 2021, Номер 8

Опубликована: Июль 20, 2021

Marine sponges have been successful in their expansion across diverse ecological niches around the globe. Pioneering work attributed this success to both a well-developed aquiferous system that allowed for efficient filter feeding on suspended organic matter and presence of microbial symbionts can supplement host heterotrophic with photosynthate or dissolved carbon. We now know sponge-microbe interactions are host-specific, highly nuanced, provide nutritional benefits sponge. Despite these advances field, many current hypotheses pertaining evolution overly generalized; over-simplifications limit our understanding evolutionary processes shaping symbioses how they contribute modern coral reefs. To highlight state knowledge we start seminal papers review contemporary using higher resolution techniques has complemented challenged early hypotheses. outline different schools thought by discussing evidence symbiont contribution divergence convergence, specificity plasticity, allopatric sympatric speciation. Based synthesis, conclude pressures complex, influences from external (nutrient limitation competition) internal (fitness trade-offs constraints) factors. recent controversies place into broader framework. Finally, propose areas future research believe will lead important new developments field.

Язык: Английский

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New Insights Into the Archaeal Consortium of Tropical Sponges

Frontiers in Marine Science, Год журнала: 2020, Номер 6

Опубликована: Янв. 7, 2020

Archaea is a poorly studied domain associated with sponges. Many questions that have been addressed for bacteria still remain largely unknown archaea. In this study, we analysed the archaeal communities of 17 tropical sponge species from Nha Trang Bay (Vietnam) using archaea specific primers. We recorded patterns diversity and spatial stability these microbial compared results obtained bacterial communities, already reported in our previous study. study species, Shannon was always lower than communities. The differences alpha as well presence indicator phyla High Microbial Abundance (HMA) Low (LMA) could not be confirmed Host identity main factor structuring assemblages. Sponge core formed by few but very abundant ZOTUs, which contribute high proportion to relative abundance. inclusion sequences into phylogenetic trees allowed finding out whether or they belonged so-called clusters (SC). Our showed most Thaumarchaeota Euryarchaeota were more closely related environmental samples clusters, suggesting might acquired seawater, need verified. However, representatives Woesarchaeota, major members microbiome two monophyletic tree, distantly any known sequence.

Язык: Английский

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