Research in Biotechnology and Environmental Science,
Год журнала:
2024,
Номер
3(2), С. 18 - 22
Опубликована: Июнь 25, 2024
Introduction:
Microbes
of
sponges
have
diverse
associations,
including
true
symbiosis.
Sponges,
being
evolutionarily
ancient
sessile
filter
feeders,
host
and
abundant
microbial
species
that
play
crucial
roles
in
metabolism.
Although
the
symbionts
are
widely
distributed
within
organism
(up
to
40%
their
volume),
ecological
relationships
interactions
between
bacteria
sponge
remain
largely
unexplored
for
many
species.
The
present
study
was
one
first
attempts
isolate
symbiotic
from
Raspaciona
aculeata.
Materials
Methods:
After
isolation
on
marine
agar
medium,
isolates
were
characterized
different
colony
morphology.
16S
rDNA
taxonomic
analysis
carried
out
isolates.
Results:
Following
an
incubation
period
two
weeks
at
25°C,
only
13
bacterial
strains
isolated
with
a
very
low
rate
genetic
biodiversity.
All
belonged
Gammaproteobacteria
class
(Pseudomonadaceae
family),
except
(isolate
AL-18ra)
belonging
Bacilli
(Bacillaceae
family).
Conclusion:
obtained
results
great
importance
advancing
understanding
symbiosis
phenomena
aculeata
its
bioapplication
potential.
Biomedical Research and Therapy,
Год журнала:
2020,
Номер
7(2), С. 3633 - 3644
Опубликована: Фев. 29, 2020
Introduction:
Proteases
are
enzymes
that
catalyze
the
hydrolysis
of
peptide
bonds
and
play
an
important
role
in
almost
all
biological
processes.
However,
excessive
protein
proteolysis
can
be
implicated
several
diseases,
such
as
cancer,
well
cardiovascular,
inflammatory,
neurodegenerative,
bacterial,
viral
parasitic
diseases.
In
these
cases,
protease
inhibitors
used
one
versatile
tools
for
regulating
proteolytic
activity
target
proteases
therapeutic
applications.
this
study,
we
expressed
characterized
a
new
serine
inhibitory
(PI-QT)
from
metagenome
sponge-associated
microorganisms
Escherichia
coli.
Methods:
The
gene
PI-QT
encoding
was
E.
coli
BL21(DE3).
addition,
purified
characterized.
Results:
Optimization
expression
recombinant
showed
suitable
conditions
were
pre-induction
cell
density
(OD600)
0.6
-
0.7,
IPTG
concentration
1
mM
temperature
25oC.
also
identified
by
mass
spectrometry
LC-MS/MS.
against
trypsin
anda-chymotrypsin
with
values
97526
U/mg
41714
U/mg,
respectively.
Maximum
obtained
at
pH
7
20-35oC.
inhibitor
stable
over
4-9
up
to
50oC.
Addition
Zn2+,
Mg2+
Ca2+
enhanced
activity,
whereas
other
metal
ions,
surfactants
oxidants
reduced
inhibitor.
Conclusion:
is
potential
Journal of Marine Science and Engineering,
Год журнала:
2020,
Номер
8(8), С. 594 - 594
Опубликована: Авг. 8, 2020
Bacteria
associated
with
invertebrates
are
considered
as
good
sources
for
biologically
active
compounds.
Sponge-derived
bacteria
were
screened
antimicrobial
activities,
the
presence
of
cluster
genes
polyketide
synthases
(PKSs)
and
non-ribosomal
peptide
synthetases
(NRPSs),
through
a
colony
picking
method.
Crude
extracts
broth
cultures
tested
microbial
inhibition.
Eleven
out
25
isolated
strains
showed
inhibition
at
least
one
eight
indicator
microorganisms.
Antimicrobial
activities
observed
in
coded
HM5,
HM6,
HM9
NRPS
PKSII
genes,
whereas
isolate
HM21
held
both
inhibited
only
growth
Bacillus
subtilis
by
well
diffusion
method
Serratia
marcescens
Two
isolates,
HM5
belonged
to
species
Bacillus.
Interestingly,
was
nearest
Streptomyces
mexicanusT
NBRC100915
(GenBank
accession
number
AB249966)
94%
sequence
similarity.
This
potent
strain
could
possibly
be
new
source
bioactive
compound
discovery.
Some
isolates
NRPS/PKS
but
did
not
exhibit
activity.
Thus,
we
suggested
that
molecular
traditional
methods
should
conducted
screening
producers.
Invertebrate Biology,
Год журнала:
2020,
Номер
139(3)
Опубликована: Авг. 8, 2020
Abstract
Sponges
play
important
roles
in
marine
ecosystems
by
contributing
to
habitat
complexity
and
benthopelagic
coupling
of
nutrients.
Yet,
the
reproduction
settlement
behaviors
diverse
sponge
species
are
not
well
understood.
Here,
we
examined
brooding
demosponge
Haliclona
amboinensis
,
which
is
common
on
shallow
reefs
Bolinao,
northwestern
Philippines.
Gravid
sponges
were
found
between
months
May
August,
coinciding
with
warmer
sea
surface
temperature.
released
parenchymella
larvae
from
brood
chambers
mid‐morning,
suggesting
that
light
temperature
may
serve
as
cues
initiate
hatching.
Larvae
immediately
swam
toward
upon
emergence
migrated
bottom
tanks
1–2
hr
after
release.
The
presence
crustose
coralline
algae
induced
high
larval
settlement.
Metamorphosis
proceeded
rapidly
vitro,
cells
spreading
laterally
substrate.
osculum
was
first
visible
at
3
days
short
pelagic
duration
H.
promotes
local
recruitment
be
for
maintenance
populations
face
disturbances.
PeerJ,
Год журнала:
2022,
Номер
10, С. e13133 - e13133
Опубликована: Апрель 6, 2022
Sponge
microbiomes
are
essential
for
the
function
and
survival
of
their
host
produce
biologically
active
metabolites,
therefore,
they
ideal
candidates
ecological,
pharmacologic
clinical
research.
Next-generation
sequencing
(NGS)
has
revealed
that
many
factors,
including
environment
host,
determine
composition
structure
these
symbiotic
communities
but
controls
this
variation
not
well
described.
This
study
assessed
microbial
associated
with
two
marine
sponges
genera
Aplysina
(Nardo,
1834)
Ircinia
1833)
in
rocky
reefs
from
Punta
Arena
de
la
Ventana
(Gulf
California)
Pichilingue
(La
Paz
Bay)
coast
Baja
California
Sur,
México
to
relative
importance
structuring
microbiome
sponges.
Specimens
sp
were
collected
by
scuba
diving
at
10
m
2
m;
samples
m.
DNA
sponge-associated
prokaryotes
was
extracted
1
cm3
tissue,
purified
sent
16S
amplicon
sequencing.
Primer
trimmed
pair-ended
rDNA
gene
sequences
merged
using
Ribosomal
Database
Project
(RDP)
Paired-end
Reads
Assembler.
Chao1,
Shannon
Simpson
(alpha)
biodiversity
indices
estimated,
as
permutational
analysis
variance
(PERMANOVA),
Bray-Curtis
distances.
The
most
abundant
phyla
differed
between
hosts.
Those
were:
Proteobacteria,
Acidobacteria,
Cyanobacteria,
Chloroflexi,
Actinobacteria,
Bacteroidetes,
Planctomycetes.
In
dominant
phylum
Acidobacteria.
Depth
main
factor
influencing
community,
similarities
(ANOSIM)
showed
a
significant
difference
different
depths.
Microbial
diversity
depth
more
important
than
microbiome.
observation
contrast
previous
reports
sponge
is
highly
specific.
Research in Biotechnology and Environmental Science,
Год журнала:
2024,
Номер
3(2), С. 18 - 22
Опубликована: Июнь 25, 2024
Introduction:
Microbes
of
sponges
have
diverse
associations,
including
true
symbiosis.
Sponges,
being
evolutionarily
ancient
sessile
filter
feeders,
host
and
abundant
microbial
species
that
play
crucial
roles
in
metabolism.
Although
the
symbionts
are
widely
distributed
within
organism
(up
to
40%
their
volume),
ecological
relationships
interactions
between
bacteria
sponge
remain
largely
unexplored
for
many
species.
The
present
study
was
one
first
attempts
isolate
symbiotic
from
Raspaciona
aculeata.
Materials
Methods:
After
isolation
on
marine
agar
medium,
isolates
were
characterized
different
colony
morphology.
16S
rDNA
taxonomic
analysis
carried
out
isolates.
Results:
Following
an
incubation
period
two
weeks
at
25°C,
only
13
bacterial
strains
isolated
with
a
very
low
rate
genetic
biodiversity.
All
belonged
Gammaproteobacteria
class
(Pseudomonadaceae
family),
except
(isolate
AL-18ra)
belonging
Bacilli
(Bacillaceae
family).
Conclusion:
obtained
results
great
importance
advancing
understanding
symbiosis
phenomena
aculeata
its
bioapplication
potential.
