Biomedicines,
Год журнала:
2022,
Номер
10(11), С. 2764 - 2764
Опубликована: Окт. 31, 2022
The
progress
of
medical
technology
and
scientific
advances
in
the
field
anticancer
treatment
have
increased
survival
probabilities
duration
life
patients.
However,
cancer-therapy-induced
cardiac
dysfunction
remains
a
clinically
salient
problem.
Effective
therapies
may
eventually
induce
cardiomyopathy.
To
date,
several
studies
focused
on
mechanisms
underlying
cancer-treatment-related
cardiotoxicity.
Cardiomyocyte
cell
lines
with
no
contractile
physiological
characteristics
cannot
adequately
model
"true"
human
cardiomyocytes.
applying
cardiomyocytes
for
research
is
fraught
many
obstacles
(e.g.,
invasiveness
procedure),
there
proliferative
limitation
rodent
primary
cultures.
Human-induced
pluripotent
stem-cell-differentiated
(hiPSC-CMs),
which
can
be
produced
efficiently,
are
viable
candidates
mimicking
vitro.
We
successfully
performed
differentiation
iPSCs
to
obtain
hiPSC-CMs.
These
hiPSC-CMs
used
investigate
pathophysiological
basis
molecular
mechanism
cardiotoxicity
develop
novel
strategies
prevent
rescue
such
propose
that
as
an
vitro
drug
screening
platform
study
targeted
cancer-therapy-related
Advanced Healthcare Materials,
Год журнала:
2024,
Номер
13(21)
Опубликована: Май 2, 2024
Multicellular
organisms
exhibit
synergistic
effects
among
their
components,
giving
rise
to
emergent
properties
crucial
for
genesis
and
overall
functionality
survival.
Morphogenesis
involves
relies
upon
intricate
biunivocal
interactions
cells
environment,
that
is,
the
extracellular
matrix
(ECM).
Cells
secrete
own
ECM,
which
in
turn,
regulates
morphogenetic
program
by
controlling
time
space
presentation
of
matricellular
signals.
The
once
considered
passive,
is
now
recognized
as
an
informative
where
both
biochemical
biophysical
signals
are
tightly
orchestrated.
Replicating
this
sophisticated
highly
interconnected
media
a
synthetic
scaffold
tissue
engineering
unattainable
with
current
technology
limits
capability
engineer
functional
human
organs
vitro
vivo.
This
review
explores
limitations
organ
morphogenesis,
emphasizing
interplay
gene
regulatory
networks,
mechanical
factors,
microenvironment
cues.
In
efforts
replicate
biological
processes
barrier
such
lung
intestine,
examined.
importance
maintaining
within
native
microenvironmental
context
highlighted
accurately
organ-specific
properties.
underscores
necessity
microphysiological
systems
faithfully
reproduce
cell-native
interactions,
advancing
understanding
developmental
disorders
disease
progression.
Artificial Organs,
Год журнала:
2024,
Номер
48(7), С. 723 - 733
Опубликована: Фев. 22, 2024
The
SARS-CoV-2
pandemic
has
spurred
an
unparalleled
scientific
endeavor
to
elucidate
the
virus'
structure,
infection
mechanisms,
and
pathogenesis.
Two-dimensional
culture
systems
have
been
instrumental
in
shedding
light
on
numerous
aspects
of
COVID-19.
However,
these
vitro
lack
physiological
complexity
comprehend
process
explore
treatment
options.
Three-dimensional
(3D)
models
proposed
fill
gap
between
2D
cultures
vivo
studies.
Specifically,
spheroids,
composed
lung
cell
types,
suggested
for
studying
serving
as
a
drug
screening
platform.
Medical Journal of Western Black Sea,
Год журнала:
2024,
Номер
8(2), С. 104 - 112
Опубликована: Авг. 30, 2024
Acute
respiratory
distress
syndrome
(ARDS)
is
a
serious
pulmonary
reaction
with
well-defined
clinical
parameters
in
humans
triggered
by
many
causes
besides
bacterial
and
viral
pneumonia.
However,
there
no
definitive
definition
of
ARDS
the
experimental
animal
model.
With
its
2010
workshop
report,
American
Thoracic
Society
defined
main
histopathological
features
that
determine
presence
laboratory
animals,
such
as
changes
parenchymal
tissue,
altered
integrity
alveolar
capillary
barrier,
inflammation,
abnormal
lung
function.
Understanding
these
parameters,
scoring
tissue
lesions
used
to
convert
observational
pathological
data
into
semi-quantitative
or
quantitative
for
statistical
analysis
improved
precision.
Materials Today Bio,
Год журнала:
2023,
Номер
24, С. 100905 - 100905
Опубликована: Дек. 7, 2023
Severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV2),
characterized
by
its
high
fatality
rate
and
contagious
nature,
has
led
to
significant
morbidity
mortality
worldwide,
significantly
impacting
both
our
daily
lives
public
health.
The
pathway
serves
as
the
primary
route
for
SARS-CoV2
propagation
within
human
body,
with
lung
acting
initial
target
organ.
Simultaneously,
functions
a
protective
barrier,
preventing
entry
of
viruses
into
bloodstream
through
alveolar-capillary
barrier.
Bioengineered
microfluidic
chips,
utilizing
advanced
near-to-native
technologies,
offer
novel
perspective
comprehending
intricate
workings
lungs
facilitating
discovery
anti-coronavirus
drugs
combat
challenges
posed
disease
2019
(COVID-19).
This
review
aims
introduce
key
elements
design
types
artificial
chips
that
closely
resemble
in
vivo-like
niches
terms
structure
function.
Furthermore,
quantitative
qualitative
techniques
evaluating
functionality
barrier
are
summarized,
confirming
successful
construction
chip
systems
engineering
approaches.
prospects
persistent
associated
establishing
next-generation
models
meet
demands
virology
studies
also
discussed.
In vitro models,
Год журнала:
2023,
Номер
2(6), С. 249 - 262
Опубликована: Июнь 29, 2023
Human
induced
pluripotent
stem
cell
(hiPSC)-derived
lung
types
such
as
alveolar
epithelial
cells
are
promising
for
toxicological
and
pharmaceutical
in
vitro
screenings.
Reproducible
differentiation
processes
highly
demanded,
but
protocols
which
suitable
the
high-throughput
generation
of
from
hiPSCs
lacking.
In
this
study,
a
new
approach
hiPSC-differentiation
epithelial-like
type
2
under
dynamic
3D-conditions
suspension
bioreactor
is
presented.
Gene
protein
expression
analyses
key
markers
during
embryonal
development
have
been
performed
comparison
to
differentiated
static
2D-conditions
evaluate
efficacy
bioreactor-based
approach.
Finally,
resulting
were
infected
by
SARS-CoV-2
pseudotypes
demonstrate
their
functionality
suitability
e.g.
COVID-19
drug
development.
The
differentiate
spheroids,
express
relevant
each
developmental
stage
on
gene
level.
3D
method
able
significantly
increase
some
conventional
2D
differentiation.
3D-differentiated
functional
receptors
can
display
viral
infection.
presented
3D-differentiation
promising,
generate
source
models.
online
version
contains
supplementary
material
available
at
10.1007/s44164-023-00052-1.
Biomedicines,
Год журнала:
2023,
Номер
11(11), С. 3034 - 3034
Опубликована: Ноя. 12, 2023
Lung
diseases
rank
third
in
terms
of
mortality
and
represent
a
significant
economic
burden
globally.
Scientists
have
been
conducting
research
to
better
understand
respiratory
find
treatments
for
them.
An
ideal
vitro
model
must
mimic
the
vivo
organ
structure,
physiology,
pathology.
Organoids
are
self-organizing,
three-dimensional
(3D)
structures
originating
from
adult
stem
cells,
embryonic
lung
bud
progenitors,
cells
(ESCs),
induced
pluripotent
(iPSCs).
These
3D
organoid
cultures
may
provide
platform
exploring
tissue
development,
regulatory
mechanisms
related
repair
epithelia,
pathophysiological
immunomodulatory
responses
different
conditions,
screening
compounds
new
drugs.
To
create
organoids
vitro,
both
co-culture
feeder-free
methods
used.
However,
there
exists
substantial
heterogeneity
culture
methods,
including
sources
AT2
media
composition,
feeder
cell
origins.
This
article
highlights
currently
available
growing
prospective
improvements
improve
techniques/conditions.
Further,
we
discuss
various
applications,
particularly
those
aimed
at
modeling
human
distal
therapy.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Апрель 3, 2023
Abstract
Human
lung
organoids
(HLOs)
are
increasingly
used
to
model
development
and
infectious
diseases,
however
their
ability
recapitulate
functional
pulmonary
tissue
response
nanomaterial
(NM)
exposures
has
yet
be
demonstrated.
Here,
we
established
a
organoid
exposure
that
utilises
microinjection
present
NMs
into
the
lumen
of
organoids.
Our
assures
efficient,
reproducible
controllable
apical
epithelium,
emulating
real-life
human
scenario.
By
comparing
impact
two
well
studied
carbon-based
NMs,
graphene
oxide
sheets
(GO)
multi-walled
carbon
nanotubes
(MWCNT),
validated
as
tools
for
predicting
NM-driven
responses.
In
agreement
with
in
vivo
data,
demonstrate
MWCNT,
but
not
GO,
elicit
adverse
effects
on
organoids,
leading
pro-fibrotic
phenotype.
findings
reveal
capacity
suitability
HLOs
hazard
assessment
aligned
much
sought-out
3Rs
(animal
research
replacement,
reduction,
refinement)
framework.
