Synthetic and Systems Biotechnology, Год журнала: 2024, Номер 10(1), С. 194 - 206
Опубликована: Окт. 28, 2024
Язык: Английский
ACS Omega, Год журнала: 2024, Номер 9(25), С. 26838 - 26862
Опубликована: Июнь 10, 2024
In the rapidly evolving landscape of nanomedicine, aptamers have emerged as powerful molecular tools, demonstrating immense potential in targeted therapeutics, diagnostics, and drug delivery systems. This paper explores computational features highlighting their advantages over antibodies, including selectivity, low immunogenicity, a simple production process. A comprehensive overview aptamer development process, specifically Systematic Evolution Ligands by Exponential Enrichment (SELEX) sheds light on intricate methodologies behind selection. The historical evolution diverse applications nanomedicine are discussed, emphasizing pivotal role delivery, precision medicine therapeutics. Furthermore, we explore integration artificial intelligence (AI), machine learning (ML), Internet Things (IoT), Medical (IoMT), nanotechnology aptameric development, illustrating how these cutting-edge technologies revolutionizing selection optimization for tailored biomedical applications. also discusses challenges methods advancing aptamers, reliable prediction models, extensive data analysis, multiomics incorporation. It addresses ethical concerns restrictions related to AI IoT use research. examines progress computer simulations nanomedicine. By elucidating importance understanding superiority exploring context challenges, this review serves valuable resource researchers practitioners aiming harness full field
Язык: Английский
Процитировано
6
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(22), С. 12284 - 12284
Опубликована: Ноя. 15, 2024
RNA therapeutics have undergone remarkable evolution since their inception in the late 1970s, revolutionizing medicine by offering new possibilities for treating previously intractable diseases. The field encompasses various modalities, including antisense oligonucleotides (ASOs), small interfering RNAs (siRNAs), microRNAs (miRNAs), and messenger (mRNAs), each with unique mechanisms applications. foundation was laid 1978 discovery that synthetic could inhibit viral replication, followed pivotal developments such as interference's 1998. COVID-19 pandemic marked a crucial turning point, demonstrating potential of mRNA vaccines accelerating interest RNA-based approaches. However, significant challenges remain, stability issues, delivery to target tissues, off-target effects, immunogenicity concerns. Recent advancements chemical modifications, systems, integration AI technologies are addressing these challenges. has seen notable successes, approved treatments spinal muscular atrophy hereditary transthyretin-mediated amyloidosis. Looking ahead, show promise personalized approaches, particularly genetic disorders cancer. continued this field, driven technological innovations deeper understanding biology, suggests transformative impact on future medical treatments. purpose review is provide comprehensive overview evolution, current state, prospects therapeutics.
Язык: Английский
Процитировано
6
Food Bioscience, Год журнала: 2025, Номер unknown, С. 105911 - 105911
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Gut, Год журнала: 2025, Номер unknown, С. gutjnl - 331742
Опубликована: Фев. 23, 2025
RNA-based therapeutics have rapidly emerged over the past decade, offering a new class of medicines that differ significantly from conventional drugs. These therapies can be programmed to target or restore defective genes, allowing for more personalised treatments and reducing side effects. Notably, RNA made significant progress in treatment genetic liver diseases, exemplified by small interfering hereditary transthyretin amyloidosis, which use liver-targeting strategies such as GalNAc conjugation improve efficacy safety. gene-editing technologies, base editor prime clustered regularly interspaced short palindromic repeats systems, also show promise with their ability minimise genomic rearrangements cancer risk. While offer high precision, challenges remain optimising delivery methods ensuring long-term safety efficacy. Lipid nanoparticle-mRNA therapeutics, particularly protein replacement rare gained support preclinical successes. Compared viral gene therapies, mRNA present safer profile reduced risks integration oncogene activation. However, clinical trials, especially face limitations sample sizes observation periods. Further studies, including non-human primates, will essential refining trial designs. Despite potential, costs pose challenge require cost–utility models guide pricing accessibility. Here, we discuss fundamental aspects showcase most relevant developments metabolic diseases.
Язык: Английский
Процитировано
0
Analytical Biochemistry, Год журнала: 2025, Номер unknown, С. 115844 - 115844
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
Meandros Medical And Dental Journal, Год журнала: 2025, Номер 26(1), С. 12 - 18
Опубликована: Март 20, 2025
Objective: With their excellent specificity, sensitivity, and speed of detection, aptamer-gated nanoparticles are a potential method for detecting Staphylococcus aureus. These techniques have been effectively used on variety actual samples, indicating promise real-world uses in environmental monitoring, clinical diagnostics, food safety. Materials Methods: MCM-41 were characterized using DLS, SEM FTIR techniques. Reference strains S. aureus, epidermidis, Escherichia coli used. After the synthesis fluorescence-loaded silica-coated nanoparticles, fluorescence release experiments performed dialysis membrane. Results: The particle size was determined to be 192±1.782 nm According BET analysis, particles had specific surface area 1019.37 m2/g, pore 2.42 nm, volume 0.99 cm3 g. It that nano-sized, narrow distribution, smooth, amorphous spherical shape. functionalized by amino groups via APTES reaction. FT-IR analysis determine correct conjugation. Typical bands at 690 1460 which correspond N-H bending vibrations asymmetric vibrations, respectively, emerged following grafting. Fluorescein-loaded silica with conjugate aptamer target bacteria aureus showed maximum release. In addition, approximately 70% fluorescein occurred 6 hours. At detection limits as low 164 CFU/mL PBS, rapid sensitive obtained. Conclusion: proposed biosensor offers several advantages, including response times, high specificity detection. Future studies will probably concentrate increasing technologies' decreasing broadening range applications.
Язык: Английский
Процитировано
0
Nature Communications, Год журнала: 2025, Номер 16(1)
Опубликована: Апрель 19, 2025
Язык: Английский
Процитировано
0
The Analyst, Год журнала: 2024, Номер 149(19), С. 4770 - 4788
Опубликована: Янв. 1, 2024
Aptamers are molecular recognition elements that have been extensively deployed in a wide array of applications ranging from diagnostics to therapeutics. Due their unique properties as compared antibodies, aptamers were also largely isolated during the COVID-19 pandemic for multiple purposes. Typically generated by conventional SELEX, inherent drawbacks process including time-consuming, cumbersome and resource-intensive nature catalysed move adopt
Язык: Английский
Процитировано
1
Talanta, Год журнала: 2024, Номер 281, С. 126808 - 126808
Опубликована: Сен. 8, 2024
Язык: Английский
Процитировано
1
Microchimica Acta, Год журнала: 2024, Номер 191(11)
Опубликована: Окт. 9, 2024
Язык: Английский
Процитировано
1