Antimicrobial Activity of the Peptide C14R Against Ab Initio Growing and Preformed Biofilms of Candida albicans, Candidaparapsilosis and Candidozyma auris
Biomolecules,
Год журнала:
2025,
Номер
15(3), С. 322 - 322
Опубликована: Фев. 21, 2025
Biofilms
are
the
predominant
lifeforms
of
microorganisms,
contributing
to
over
80%
infections,
including
those
caused
by
Candida
species
like
C.
albicans,
parapsilosis
and
Candidozyma
auris.
These
form
biofilms
on
medical
devices,
making
infections
challenging
treat,
especially
with
rise
in
drug-resistant
strains.
particularly
hospital-acquired
ones,
a
significant
health
threat
due
their
resistance
antifungals
risk
developing
systemic
(i.e.,
sepsis).
We
have
previously
shown
that
C14R
reduces
viability
albicans
auris,
but
not
parapsilosis.
Here,
we
show
only
inhibits
pore
formation,
resazurin
reduction
assay,
fluorescence-based
permeabilization
it
also
halts
biofilm
maturation
significantly
biomass
preformed
70%.
findings
suggest
could
be
an
effective
option
for
treating
severe
fungal
offering
potential
new
treatment
approach
biofilm-related
diseases.
Further
research
is
needed
fully
understand
its
dispersal
optimize
use
future
applications
as
antifungal
clinical
settings.
Язык: Английский
Anti-Staphylococcus aureus Activity and Structural Characterization of Rationally Designed Peptides
Antibiotics,
Год журнала:
2025,
Номер
14(5), С. 437 - 437
Опубликована: Апрель 26, 2025
Background/Objectives:
Microbial
infections
represent
a
significant
threat
to
public
health
due
the
emergence
and
spread
of
antimicrobial
resistance.
Adjunctive
alternative
therapeutic
strategies
are
explored
tackle
this
issue,
including
use
natural
or
synthetic
peptides.
Previous
research
showed
that
antibody-derived
peptides
possess
antimicrobial,
antiviral,
immunomodulatory
properties.
This
study
aimed
characterize
newly
designed
evaluate
their
effectiveness
against
representative
strains
Staphylococcus
aureus,
drug-resistant
isolates.
Methods:
Colony-forming
unit
assays
confocal
microscopy
studies
were
performed
peptide
activity
planktonic
microbial
cells.
Cytotoxicity
tests
on
THP-1
human
monocytic
Circular
dichroism
(CD)
nuclear
magnetic
resonance
(NMR)
employed
for
conformational
characterization
Results:
The
half-maximal
effective
concentrations
bacterial
reference
isolates
ranged
from
0.17
18.05
µM,
while
cytotoxic
effects
not
observed
mammalian
A
killing
kinetics
analysis
observation
by
interaction
between
bacteria
suggested
mechanism
action
involving
membrane
perturbation.
CD
all
predominantly
exhibit
random
coil
arrangement
in
aqueous
solution.
NMR
spectroscopy
revealed
most
active
adopts
helical
conformation
presence
mimetics.
Conclusions:
structural
evaluation
peptides'
may
lead
selection
candidate
be
further
studied
develop
an
treatment
caused
strains.
Язык: Английский
Therapeutic Peptides: Recent Advances in Discovery, Synthesis, and Clinical Translation
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(11), С. 5131 - 5131
Опубликована: Май 27, 2025
In
recent
decades,
peptide-based
therapeutics
have
undergone
transformative
advancements
driven
by
breakthroughs
in
production,
modification,
and
analytical
technologies.
Innovations
chemical
biological
synthesis,
coupled
with
novel
design
delivery
strategies,
systematically
addressed
historical
limitations
(e.g.,
poor
stability
bioavailability).
These
facilitated
the
characterization
clinical
translation
of
diverse
natural
engineered
peptides
across
therapeutic
domains,
including
metabolic
disorders,
oncology,
infectious
diseases.
This
review
synthesizes
critical
developments
peptide
drug
discovery,
production
technologies,
applications,
while
highlighting
emerging
challenges
opportunities.
We
further
evaluate
potential
addressing
unmet
medical
needs
propose
strategic
directions
to
accelerate
their
integration
into
precision
medicine
paradigms.
Язык: Английский