Antimicrobial Activity of the Peptide C14R Against Ab Initio Growing and Preformed Biofilms of Candida albicans, Candidaparapsilosis and Candidozyma auris

Biomolecules, Journal Year: 2025, Volume and Issue: 15(3), P. 322 - 322

Published: Feb. 21, 2025

Biofilms are the predominant lifeforms of microorganisms, contributing to over 80% infections, including those caused by Candida species like C. albicans, parapsilosis and Candidozyma auris. These form biofilms on medical devices, making infections challenging treat, especially with rise in drug-resistant strains. particularly hospital-acquired ones, a significant health threat due their resistance antifungals risk developing systemic (i.e., sepsis). We have previously shown that C14R reduces viability albicans auris, but not parapsilosis. Here, we show only inhibits pore formation, resazurin reduction assay, fluorescence-based permeabilization it also halts biofilm maturation significantly biomass preformed 70%. findings suggest could be an effective option for treating severe fungal offering potential new treatment approach biofilm-related diseases. Further research is needed fully understand its dispersal optimize use future applications as antifungal clinical settings.

Language: Английский

Anti-Staphylococcus aureus Activity and Structural Characterization of Rationally Designed Peptides

Antibiotics, Journal Year: 2025, Volume and Issue: 14(5), P. 437 - 437

Published: April 26, 2025

Background/Objectives: Microbial infections represent a significant threat to public health due the emergence and spread of antimicrobial resistance. Adjunctive alternative therapeutic strategies are explored tackle this issue, including use natural or synthetic peptides. Previous research showed that antibody-derived peptides possess antimicrobial, antiviral, immunomodulatory properties. This study aimed characterize newly designed evaluate their effectiveness against representative strains Staphylococcus aureus, drug-resistant isolates. Methods: Colony-forming unit assays confocal microscopy studies were performed peptide activity planktonic microbial cells. Cytotoxicity tests on THP-1 human monocytic Circular dichroism (CD) nuclear magnetic resonance (NMR) employed for conformational characterization Results: The half-maximal effective concentrations bacterial reference isolates ranged from 0.17 18.05 µM, while cytotoxic effects not observed mammalian A killing kinetics analysis observation by interaction between bacteria suggested mechanism action involving membrane perturbation. CD all predominantly exhibit random coil arrangement in aqueous solution. NMR spectroscopy revealed most active adopts helical conformation presence mimetics. Conclusions: structural evaluation peptides’ may lead selection candidate be further studied develop an treatment caused strains.

Language: Английский