International Journal of Molecular Sciences,
Год журнала:
2021,
Номер
22(15), С. 7837 - 7837
Опубликована: Июль 22, 2021
The
musculoskeletal
system
is
a
vital
body
that
protects
internal
organs,
supports
locomotion,
and
maintains
homeostatic
function.
Unfortunately,
disorders
are
the
leading
cause
of
disability
worldwide.
Although
implant
surgeries
using
autografts,
allografts,
xenografts
have
been
conducted,
several
adverse
effects,
including
donor
site
morbidity
immunoreaction,
exist.
To
overcome
these
limitations,
various
biomedical
engineering
approaches
proposed
based
on
an
understanding
complexity
human
tissue.
In
this
review,
edge
tissue
3D
bioprinting
technology
tissue-derived
decellularized
extracellular
matrix
bioink
described.
particular,
studies
in
vivo
regeneration
vitro
modeling
focused
on.
Lastly,
current
breakthroughs,
future
perspectives
Acta Biomaterialia,
Год журнала:
2024,
Номер
182, С. 67 - 80
Опубликована: Май 13, 2024
Large
skin
injuries
heal
as
scars.
Stiffness
gradually
increases
from
normal
to
scar
tissue
(20x
higher),
due
excessive
deposition
and
crosslinking
of
extracellular
matrix
(ECM)
mostly
produced
by
(myo)fibroblasts.
Using
a
custom
mold,
skin-derived
ECM
hydrogels
(dECM)
were
UV
crosslinked
after
diffusion
ruthenium
(Ru)
produce
Ru-dECM
gradient
hydrogel.
The
Ru
equates
stiffness
models
physiology
the
scarred
skin.
Crosslinking
in
results
23-fold
increase
similar
that
Collagen
fiber
density
stiffness-dependent
fashion
while
stress
relaxation
also
alters,
with
one
additional
Maxwell
element
necessary
for
characterizing
Ru-dECM.
Alignment
fibroblasts
encapsulated
suggests
directs
orientate
at
∼45
°
regions
below
120
kPa.
In
areas
above
kPa,
decrease
prior
adjusting
their
orientation.
Furthermore,
remodel
surrounding
gradient-dependent
fashion,
rearrangement
cell-surrounding
high-stiffness
areas,
formation
interlaced
collagen
bundles
low-stiffness
areas.
Overall,
this
study
shows
local
environment
generate
an
optimal
mechanical
topographical
environment.
This
developed
versatile
vitro
model
using
hydrogel
unchanged
biochemical
Ruthenium
crosslinking,
20-fold
was
achieved
observed
fibrotic
interaction
between
depends
on
changes
stiffness.
directed
alignment
∼45°
with≤
cells
higher
decreased
first
then
oriented
themselves.
remodeled
regulated
its
mechanics
reach
condition.
Our
highlights
dynamic
interplay
matrix,
shedding
light
potential
mechanisms
strategies
target
remodeling.
Abstract
3D
printing
has
greatly
improved
the
precision
of
cell
and
biomaterial
placement,
enabling
accurate
reproduction
tissue
models
with
sustainable
potential.
Various
techniques,
including
inkjet
printing,
extrusion‐based
vat
photopolymerization,
offer
unique
advantages
but
often
fail
to
replicate
full
complexity
native
tissues
because
material
scalability
limitations.
Hybrid
bioprinting,
combining
multiple
techniques
in
a
single
process,
shown
great
potential
creating
complex
multifunctional
capabilities,
ranging
from
patient‐specific
implant
fabrication
full‐scale
organ
development.
It
capitalizes
on
strengths
integration
sustainable,
renewable
biomaterials
at
varying
resolutions,
nano
microscale.
This
approach
addresses
both
biological
environmental
responsibility
by
minimizing
waste
enhancing
sustainability
engineering
processes.
Despite
progress,
substantial
gap
remains
between
current
technologies
bioengineering
requirements.
A
deep
understanding
hybrid
its
underlying
mechanisms
is
crucial.
Herein,
this
review
summarizes
discusses
recent
advancements
systems
for
fabricating
multiscale
hierarchical
models,
focusing
challenges
field.
aims
insights
identify
key
requirements
advancing
technology
toward
developing
functional,
biomimetic
constructs.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 16, 2025
Abstract
The
integration
of
biomaterials
with
living
cells
and
stimuli-responsive
materials
can
be
employed
to
create
bioinks
capable
generating
3D
in
vitro
models
that
better
recapitulate
native
tissues.
We
introduce
a
multilayered
artery
model
combines
hybrid
multifunctional
including
polymeric
ink
mimic
the
tunica
adventitia
arterial
wall,
an
extracellular
matrix
(ECM)-based
bioink
for
media
layer.
layer
integrates
inorganic
(plasmonic
nanoparticles)
organic
(polymers)
components,
providing
structural
support
introducing
diverse
functionalities
system.
cell-laden
consists
human
vascular
smooth
muscle
(vSMC)
within
hydrogel
based
on
porcine
artery-derived
decellularized
(dECM)
fosters
optimal
cell
growth
proliferation.
An
embedding
bioprinting
technique
was
fabrication
multimaterial
consisting
concentric
cylinders.
dimensions
parameters
were
fine-tuned
ensure
effective
crosslinking
multiple
layers
resulting
creation
self-supporting
constructs.
demonstrate
effectiveness
composition
supporting
viability
proliferation
construct,
expanding
possibilities
employing
novel
multi-component
vasculature
resembling
structure
blood
vessels.
International Journal of Molecular Sciences,
Год журнала:
2021,
Номер
22(15), С. 7837 - 7837
Опубликована: Июль 22, 2021
The
musculoskeletal
system
is
a
vital
body
that
protects
internal
organs,
supports
locomotion,
and
maintains
homeostatic
function.
Unfortunately,
disorders
are
the
leading
cause
of
disability
worldwide.
Although
implant
surgeries
using
autografts,
allografts,
xenografts
have
been
conducted,
several
adverse
effects,
including
donor
site
morbidity
immunoreaction,
exist.
To
overcome
these
limitations,
various
biomedical
engineering
approaches
proposed
based
on
an
understanding
complexity
human
tissue.
In
this
review,
edge
tissue
3D
bioprinting
technology
tissue-derived
decellularized
extracellular
matrix
bioink
described.
particular,
studies
in
vivo
regeneration
vitro
modeling
focused
on.
Lastly,
current
breakthroughs,
future
perspectives
